| 质粒名称: | pSY6 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 90482 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pIMP1-ptb |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | group II intron and ItrA |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSY6 was a gift from Sheng Yang (Addgene plasmid # 90482 ; http://n2t.net/addgene:90482 ; RRID:Addgene_90482) |
| 参考文献部分: | Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Shao L, Hu S, Yang Y, Gu Y, Chen J, Yang Y, Jiang W, Yang S. Cell Res. 2007 Nov;17(11):963-5. doi: 10.1038/cr.2007.91. 10.1038/cr.2007.91 PubMed 17971808 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSY6 8031 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Targeted gene disruption by use of a group II intron (targetron)
vector in Clostridium acetobutylicum.
ACCESSION .
VERSION .
KEYWORDS pSY6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8031)
TITLE Sheng Yang lab plasmids
REFERENCE 2 (bases 1 to 8031)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8031
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 67..153
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
RBS 2329..2351
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
primer_bind complement(5530..5546)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5530..5546)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5543..5565)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5554..5570
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5578..5608)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5623..5644
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5761..5778)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5932..6520)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6012..6031)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6691..7551)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7314..7333
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7552..7656)
/gene="bla"
/label=AmpR promoter
primer_bind 7724..7742
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7780..7802)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7902..7921
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
terminator 7979..8006
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
ORIGIN
1 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
61 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
121 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
181 ggggagaccc cacactacca tcggcggctt ggcgatccac aggacgggtg tggtcgccat
241 gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa
301 gcggtcggac agtgctccga gaacgggtgc tcgacattca cttgtgttta tgaatcacgt
361 gacgatgaca atgaaagcat acaacaagag ttttacgttg tttcgctatc attgccattt
421 cccatttttc tttgccttta agatttttga ccttattgac atggtgaatt tcataggaag
481 tattttcatc agacgtccca cataattcac aacatttagc ttttaacctg ttttcaagag
541 tattccgggc atagccatac aatacaggag cttgacttat ctcatccgta aattgataag
601 gggatttaca ttcactaaaa tttgcaaaat aacggcgctg cttaccttgc tttatctcat
661 acgggatccc ccacgaacca cttccatctt taaacatgga aatggttttt gaaagtgttc
721 ccttatgttt ggaggctatc gtttttagac agctgtattc cataagataa gcaaaataat
781 tgagctggtt aaaattactt gctagaccgt agtaattaca aatcccgcgg agttcagaat
841 tataaattgt gatgatttct aagtctgttg aacgaataag atatttcctg tgaactggaa
901 accatgagct atctttcttt tggatagcta ttttcttgtc aaaaataaat tgacgaattt
961 tgtcttgaag aggaataagg agttctacac tcccattgag tgttctcttt ttgactttac
1021 cagatcgttt tatcgttcca gatctcctta ctcgtatatc atatcccaga aaacgagcgg
1081 gttgactgct atgtgtgatg agtgtttttt cttcactcaa ttccattttt agcttgttat
1141 gaataaaaag ttttaattgt tcttttatcc attgacagtc ctctttgctt cctttaacag
1201 agataatgaa gtcgtccgca taccggacgt atttcaatac tttatttgtc tgtgaggtac
1261 aggggagtgt gggtaatctt ttacgttttt cttgatattc taaaagaact ttagcttttt
1321 cttcaccctc caacttcttg agacggtgag aaattctttt tatctcattg tggagctccc
1381 gatattcagg tgttattctt tctggacttt ctcggtcaaa cttcattttg agttgtaaaa
1441 caaacttatc caattcatga agatagatgt tggccaaaag aggagataga attccacctt
1501 gaggtgttcc gctgtaagtt ttgtgatact gccagttttc cagataacct gcttttagaa
1561 atttataaat caattggctc attttcatat ctttgatttt aagattgatg agtccaatga
1621 gtgtaacgtg gtctatatta tcgaagcagc cttttatatc tccctccaca aaccatcttg
1681 cgccgccaaa ctctcttttg attgttttca aagctgtgtg acagcttcgt tgaggtctaa
1741 aaccgtgaga cacatcttcg aataccggtt catagataga ttcaagaatt attctcacag
1801 cttcttggat caatttatct gtgaaagttg gaattcctaa aggtctcatc tttttagaat
1861 tcttttttgc aatatacatt cttcgtacag gttgaggata gtaagttccg tcttttaaag
1921 attgaataat cttttttatt ttttcttcac taaagccatc cgctgtatca tctaatattc
1981 cttttgtgga agctccttta ttggaatata aattttgata cgccacgtaa taaatatctg
2041 gacgtaaaag ataacgataa agtcttgtaa aaacttcgtc tatattttct tgtgaatttt
2101 tactgattct ttctaaaatt gccattgttg gtttcatttt gaggttttcc tccctaatca
2161 atttttaatt ttagtacaca ataactgtac ccctttgcca tgtaaagggc gttacccttc
2221 tcagactact acgagtgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
2281 agtgactggc gatgctgtcg gaatggacga tctagccata tatgtatatc tccttcttaa
2341 agttaaacaa aattatttct agggggctgc agaaaatgat atggtgaagt agggaggtac
2401 cgccttgttc acattactgt gactggtttg caccaccctc ttcgggaacc gtacgtaccc
2461 ctctcggagt atacggctct gttattgttc gttcgtaaaa attcactgtc gacattcact
2521 tgtgtttatg aatcacgtga cgatgacaat gaaagcatac aacaagagtt ttacgttgtt
2581 tcgctatcat tgccatttcc caacgcgtcg ccacgtaata aatatctgga cgtaaaagat
2641 aacgataaag tcttgtaaaa acttcgtcta tattttcttg tgaattttta ctgattcttt
2701 ctaaaattgc cattgttggt ttcattttga ggttttcctc cctaatcaat ttttaatttt
2761 agtacacaat aactgtaccc ctttgccatg taaagggcgt tacccttctc agactactac
2821 gagtactccg tacccttgca agattttcaa gctctagtgc tatagccttt ttcctccttt
2881 ctattaggca ttcttgttta gggtatcccc agttagtgtt aagtcttggt aaattcagat
2941 tctcggcatc gctttcgttt cgttcccata ggttctccta cagattgtac aaatgtggtg
3001 ataacagata agtccatagg cttaacttac ctttctttgt actagaggtt tcagacactt
3061 tcctctatcg accagcaacc gaaattagaa acttgcgttc agtaaacaca acttatacct
3121 tatatctgat taacattgcg actcagtcgt acccgattgt ctttaggtaa ctcatcgctt
3181 tccaaccgtg ctctgttccc gtatcagctt tcgcttttcg gttaggttgg ctgttttctg
3241 tgttatctta cagagtagta ccttaaacta cttgacttaa caccctatct gggcgcaccc
3301 tatgggctgg taaggataat tatctcgagc atatgtcgac actccctttt actatttaat
3361 tatctatgtt aaatgattaa cgtgctcatt tatattttaa caaacttttc acatgaagtt
3421 aagtttttta gaaaattatt tatattttat agaattcccg gggatccgtc gacctgcagc
3481 caagcttaat cgcatttcat agattgacct cccaataact acgtggtgtt attgggaggt
3541 caatctattt catttgcctc ttgctcaaag ttcccaaatt cgagtaagag gtatttttgt
3601 ttttggtcgt cgcctctcat tagtagttca gggtttaaca ttaatactcc agtttttctt
3661 tttataatat ttccttcttc taagatttta agtgttgtta ttactgtttg tagacttgtt
3721 cctgtagctt ttgctatttc tcttgttgta gctatcattg tattgttact taagtggaca
3781 ttatctagga tatagttaac gattttaagt ttttttccgc caatcatatc taacatactt
3841 attaattgca ctatatatgc ctttacgaag ttaccagacg tttgtttacg gtataacttg
3901 tctacctcta tgacttctcc actttcttcg tctatgagcc tctgagagcc tttatagact
3961 gttccatatc tttctttcat ctttttctca ctccttattt taaactattc taactatatc
4021 ataactgttc taaaaaaaaa agaacatttg ttaaaagaaa ttagaacaaa atgagtgaaa
4081 aattagaaca aacaaattcc ttataaacct tatcatctca acctatatta agattttacc
4141 tagttgaatc ttcttttcta tataaagcgt cggagcatat cagggggtta tctaacgtaa
4201 atgctaccct tcggctcgct ttcgctcggc attgacgtca gatactgcac cccctgaacc
4261 cccatgctcc aacagcaaaa aggaaacttt ttgctgcttt tccgacgctt attcgcttcg
4321 ctcatattta tatagaaaag aagtgaatgc gcaaaagaca taatcgattc acaaaaaata
4381 ggtacacgaa aaacaagtta agggatgcag tttatgcatc ccttaactta cttattaaat
4441 aatttatagc tattgaaaag agataagaat tgttcaaagc taatattgtt taaatcgtca
4501 attcctgcat gttttaagga attgttaaat tgattttttg taaatatttt cttgtattct
4561 ttgttaaccc atttcataac gaaataatta tacttctgtt tatctttgtg tgatattctt
4621 gatttttttc tatttaatct gataagtgag ctattcactt taggtttagg atgaaaatat
4681 tctcttggaa ccatacttaa tatagaaata tcaacttctg ccattaaaaa taatgccaat
4741 gagcgttttg tatttaataa tcttttagca aacccgtatt ccacgattaa ataaatctca
4801 tcagctatac tatcaaaaac aattttgcgt attatatccg tacttatgtt ataaggtata
4861 ttaccaaata ttttatagga ttggttttta ggaaatttaa actgcaatat atccttgttt
4921 aaaacttgga aattatcgtg atcaacaagt ttattttctg tagttttgca taatttatgg
4981 tctatttcaa tggcagttac gaaattacac ctctgtacta attcaagggt aaaatgccct
5041 tttcctgagc cgatttcaaa gatattatca tgttcattta atcttatatt tgtcattatt
5101 ttatctatat tatgttttga agtaataaag ttttgactgt gttttatatt tttctcgttc
5161 attataaccc tctttatttt ttcctcctta taaaattagt ataattatag cacgagctct
5221 gataaatatg aacatgatga gtgatcgtta aatttatact gcaatctgat gcgattattg
5281 aataaaagat atgagagatt tatctagttt ctttttttac aagaaaaaag aaagttctta
5341 aaggttttat acttttggtc gtagagcaca cggtttaacg acttaattac gaagtaaata
5401 agtctagtgt gttagacttt aatgtttttt taaggcatta gtgcatttaa gcgtcagagc
5461 atggctttat gccgagaaaa ctattggttg gaatggcgtg tgtgttagcc aaagcttggc
5521 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
5581 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
5641 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
5701 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
5761 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
5821 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
5881 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
5941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
6001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
6061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
6121 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
6181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
6241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
6301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
6361 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
6421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
6481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
6541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
6601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
6661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
6721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
6781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
6841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
6901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
6961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
7021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
7081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
7141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
7201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
7261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
7321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
7381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
7441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
7501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
7561 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
7621 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
7681 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
7741 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
7801 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
7861 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
7921 actgagagtg caccatatgc ttgtagatat gacgacagga agagtttgta gaaacgcaaa
7981 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt a
//
