pLKO-DEST-puro

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

Stbl3
出品公司: Addgene
载体名称: pLKO-DEST-puro
质粒类型: 慢病毒载体;RNAi载体;cDNA/shRNA gateway
高拷贝/低拷贝: 低拷贝
克隆方法: gateway
启动子: U6
载体大小: 8886 bp
5' 测序引物及序列: LKO.1 5' :GACTATCATATGCTTACCGT
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素、氯霉素
筛选标记: Puromycin
克隆菌株: 阳性筛选用DB3.1 ,阴性筛选用OmniMAX2-T1, 重组慢病毒载体的复制用Stbl3感受态细胞,以维持质粒的稳定性。
宿主细胞(系): 哺乳动物细胞
备注:
其它筛选标记 pLKO-DEST-EGFP
                        pLKO-DEST-YFP
                        pLKO-DEST-hygro
 
产品目录号: #32682
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLKO-DEST-puro载体图谱



载体简介

载体序列

LOCUS       pLKO-DEST-puro	8886 bp 	DNA	SYN
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="pLKO.DEST.puro"
                     /mol_type="other DNA"
     misc_feature    34..49
                     /label="cPPT"
     CDS             complement(115..729)
                     /label="ORF frame 1"
                     /translation="MVSSGGSPWGERSVIRSTREPTADVRSGGLQNAEHRAGRNRAHT
                     TAPHPASRTAPSRPLLSARPAEQPLLATAHRGRRAAIAPWRCPSARVLVRRAASVCHV
                     RHAANRKEPSRLRGGAGSVAGGPTRVAAKIRVTLRTDVKNVRDPGSAPLRFPEPRPEQ
                     PRPCANPGLPWKRRNPNPLEPRPNWISAVPVINPKILNFCNLFL*"
     CDS             488..1327
                     /label="ORF frame 2"
                     /translation="MAARRPRWAVANSGCSAGRAESSGREGAVREAGCGAVVWALFLP
                     ARCSAFCKPPERTSAVGSLVDRITDLSPQGDPPELTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     CDS             complement(533..1378)
                     /label="ORF frame 3"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     gene            728..1327
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    1438..1459
                     /label="U3PPT"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    1438..1453
                     /label="cPPT"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    1455..1507
                     /label="delta_U3"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    1508..1688
                     /label="HIV-1_5_LTR"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    1508..1688
                     /label="truncHIV-1_3_LTR"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     terminator      1763..1882
                     /label="SV40_PA_terminator"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    1851..1870
                     /label="EBV_rev_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     rep_origin      1900..1998
                     /label="SV40_origin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    1983..2002
                     /label="SV40pro_F_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     promoter        complement(2077..2095)
                     /label="T7_promoter"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     promoter        complement(2105..2121)
                     /label="M13_forward20_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    complement(2114..2136)
                     /label="M13_pUC_fwd_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    2114..2262
                     /label="lacZ_a"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     rep_origin      2280..2586
                     /label="f1_origin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     promoter        2779..2807
                     /label="AmpR_promoter"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     gene            2849..3709
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     CDS             2849..3709
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      3864..4483
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        4870..4899
                     /label="lac_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4913..4935
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        4934..4952
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        4969..4988
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5242..5422
                     /label="HIV-1_5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5242..5422
                     /label="truncHIV-1_3_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5533..5577
                     /label="HIV-1_psi_pack"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             5965..6822
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    6087..6320
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     promoter        6856..7088
                     /label="hU6_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    7018..7037
                     /label="LKO1_5_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    7132..7256
                     /label="attR1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    complement(7156..7256)
                     /label="attR2"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     promoter        7281..7304
                     /label="lac_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     gene            7365..8024
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     CDS             7365..8024
                     /label="ORF frame 3"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    8366..8671
                     /label="ccdB"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(8712..8819)
                     /label="attR1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    8712..8812
                     /label="attR2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     CDS             8839..456
                     /label="ORF frame 1"
                     /translation="MGGRSRGPKLTREFVDSRQMAVFIHNFKRKGGIGGYSAGERIVD
                     IIATDIQTKELQKQITKIQNFRVYYRDSRDPVWPRLEGVGVAPFPRQPWVCAGTRLLW
                     AWFRETQRRRPWVSHILHVRSQRHPDLRRYPCGPPGDASCSAPKSGRFLAVRGVPDVT
                     NGSRTSH*"
ORIGIN
    1 tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
   61 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
  121 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
  181 tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
  241 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
  301 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
  361 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
  421 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
  481 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
  541 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
  601 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
  661 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga
  721 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
  781 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
  841 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
  901 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
  961 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
 1021 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
 1081 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
 1141 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
 1201 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
 1261 caccgccgac gtcgagGtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
 1321 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
 1381 atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
 1441 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
 1501 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
 1561 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
 1621 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
 1681 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
 1741 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
 1801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
 1861 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
 1921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
 1981 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
 2041 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
 2101 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
 2161 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
 2221 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
 2281 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
 2341 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
 2401 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
 2461 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
 2521 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
 2581 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
 2641 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
 2701 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
 2761 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
 2821 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
 2881 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
 2941 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
 3001 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
 3061 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
 3121 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
 3181 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
 3241 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
 3301 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
 3361 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
 3421 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
 3481 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
 3541 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
 3601 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
 3661 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
 3721 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
 3781 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
 3841 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
 3901 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
 3961 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
 4021 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
 4081 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
 4141 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
 4201 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
 4261 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
 4321 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
 4381 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
 4441 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
 4501 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
 4561 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
 4621 cagcgagtca gtgagcgagg aagcggaaga gcgccctttg agtgagctga taccgctcgc
 4681 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
 4741 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
 4801 cccgactgga aagcgggcag tgagcgcaac gcaaaatgtg agttagctca ctcattaggc
 4861 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
 4921 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
 4981 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
 5041 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
 5101 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
 5161 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
 5221 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
 5281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
 5341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
 5401 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
 5461 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
 5521 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
 5581 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
 5641 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
 5701 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
 5761 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
 5821 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
 5881 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
 5941 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
 6001 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
 6061 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
 6121 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
 6181 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
 6241 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
 6301 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
 6361 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
 6421 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
 6481 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
 6541 gcaagtttgt ggaattggtt taacataacC aattggctgt ggtatataaa attattcata
 6601 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
 6661 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
 6721 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
 6781 cgattagtga acggatctcg acggtatcga tcACGAGACT AGCCTCGAGC GGCCGCCCCC
 6841 TTCACCGAGG GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT
 6901 AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA
 6961 CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC
 7021 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG
 7081 AAAGGACGAA ACACCGGTCC GCAGGTATGC ACGCGATCct cgagcccatc aacaagtttg
 7141 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
 7201 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
 7261 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
 7321 tgagttagga tccgTcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
 7381 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
 7441 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
 7501 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
 7561 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
 7621 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
 7681 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
 7741 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
 7801 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
 7861 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
 7921 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
 7981 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
 8041 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
 8101 atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac
 8161 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
 8221 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
 8281 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
 8341 gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag
 8401 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
 8461 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
 8521 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
 8581 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
 8641 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
 8701 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
 8761 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
 8821 tcttgtacaa agtggttgat gggcggccgc tctagagggc ccaagcttac gcgtgaattc
 8881 gtcgac
//

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