| 质粒名称: | FG12 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 14884 |
| 存储实验室: | David Baltimore |
|
载体骨架基本信息 |
|
| 载体名称: | HR'CS-G |
| 载体出品公司: | I. Verma, Salk |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | flap-Ub promoter-GFP-WRE |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | na |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | FG12 was a gift from David Baltimore (Addgene plasmid # 14884 ; http://n2t.net/addgene:14884 ; RRID:Addgene_14884) |
| 参考文献部分: | Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Qin XF, An DS, Chen IS, Baltimore D. Proc Natl Acad Sci U S A. 2003 Jan 7. 100(1):183-8. 10.1073/pnas.232688199 PubMed 12518064 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FG12 9994 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FG12
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9994)
AUTHORS Qin XF, An DS, Chen IS, Baltimore D
TITLE Inhibiting HIV-1 infection in human T cells by lentiviral-mediated
delivery of small interfering RNA against CCR5.
JOURNAL Proc Natl Acad Sci U S A. 2003 Jan 7. 100(1):183-8.
PUBMED 12518064
REFERENCE 2 (bases 1 to 9994)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9994
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 616..814
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 832..1012
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1059..1184
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1677..1910
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2437..2554
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2652..3863
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3700..3719
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
regulatory 3905..3914
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3911..4630
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3956..3977)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4217..4236)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4564..4585
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4679..5267
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4732..4752)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5150..5161)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5270..5286)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5271..5287)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5796..5976
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6002..6019)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6008..6232
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6278..6706
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6365..6384)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6575..6596
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6715..6735)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6720..7049
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6900..7035
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6962..6981
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7097..7144
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7163..7537
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7667..7788
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7704..7723)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7758..7777
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7837..7853)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7837..7853)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7850..7872)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7861..7877
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7885..7915)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7930..7951
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8068..8085)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8239..8827)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8319..8338)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8998..9858)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9621..9640
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9859..9963)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
2401 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
2461 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
2521 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
2581 tccagtttgg ttaattctag acagttaaca tctcgagcca taaagatggt taattaaccc
2641 acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
2701 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
2761 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
2821 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
2881 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
2941 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
3001 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
3061 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
3121 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
3181 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
3241 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
3301 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
3361 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
3421 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
3481 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
3541 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
3601 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
3661 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
3721 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
3781 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
3841 cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatctcc accccgtacc
3901 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
3961 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
4021 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
4081 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
4141 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
4201 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
4261 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
4321 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
4381 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
4441 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
4501 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
4561 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
4621 gtacaagtaa agcggccgcg actctagtcc ctcccaattc gatatcaagc ttatcgataa
4681 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
4741 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
4801 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
4861 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
4921 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
4981 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
5041 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
5101 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
5161 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
5221 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
5281 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
5341 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
5401 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
5461 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
5521 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
5581 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
5641 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
5701 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
5761 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
5821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
5881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
5941 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
6001 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
6061 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
6121 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
6181 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
6241 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
6301 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
6361 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
6421 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
6481 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
6541 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
6601 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
6661 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
6721 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
6781 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
6841 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
6901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
6961 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
7021 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
7081 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
7141 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
7201 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
7261 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
7321 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
7381 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
7441 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
7501 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
7561 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
7621 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
7681 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
7741 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
7801 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
7861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
7921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
7981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
8041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
8101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
8161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
8221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
8281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
8341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
8401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
8461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
8521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
8581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
8641 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
8701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
8761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
8821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
8881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
8941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
9001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
9061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
9121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
9181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
9241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
9301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
9361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
9421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
9481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
9541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
9601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
9661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
9721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
9781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
9841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
9901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
9961 gcgcacattt ccccgaaaag tgccacctga cgtc
//
