pLKO-DEST-hygro

LOCUS       pLKO-DEST-hygro	9176 bp 	DNA	SYN
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="pLKO.DEST.hygro"
                     /mol_type="other DNA"
     misc_feature    76..91
                     /label="cPPT"
     misc_feature    complement(535..555)
                     /label="pBABE_3_primer"
     misc_feature    complement(541..757)
                     /label="SV40_enhancer"
     promoter        553..822
                     /label="SV40_promoter"
     rep_origin      721..798
                     /label="SV40_origin"
     misc_feature    783..802
                     /label="SV40pro_F_primer"
     CDS             complement(856..1677)
                     /label="ORF frame 3"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHHVLIRSENGYTSSRELFAKA*"
     CDS             919..1944
                     /label="ORF frame 1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            934..1941
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     terminator      2077..2196
                     /label="SV40_PA_terminator"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2165..2184
                     /label="EBV_rev_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2286..2301
                     /label="cPPT"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2286..2307
                     /label="U3PPT"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2303..2355
                     /label="delta_U3"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2356..2536
                     /label="HIV-1_5_LTR"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2356..2536
                     /label="truncHIV-1_3_LTR"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     terminator      2611..2730
                     /label="SV40_PA_terminator"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2699..2718
                     /label="EBV_rev_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     rep_origin      2748..2846
                     /label="SV40_origin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2831..2850
                     /label="SV40pro_F_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     promoter        complement(2925..2943)
                     /label="T7_promoter"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     promoter        complement(2953..2969)
                     /label="M13_forward20_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    complement(2962..2984)
                     /label="M13_pUC_fwd_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    2962..3110
                     /label="lacZ_a"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     rep_origin      3128..3434
                     /label="f1_origin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     promoter        3627..3655
                     /label="AmpR_promoter"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            3697..4557
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             3697..4557
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4677..5261
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5572..5590
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5607..5626
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5880..6060
                     /label="truncHIV-1_3_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5880..6060
                     /label="HIV-1_5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6171..6215
                     /label="HIV-1_psi_pack"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             6603..7499
                     /label="ORF frame 3"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSSSPSTSLYKKAERET*"
     misc_feature    6725..6958
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSSSPSTSLYKKAERET*"
     misc_feature    7464..7588
                     /label="attR1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSSSPSTSLYKKAERET*"
     misc_feature    complement(7488..7588)
                     /label="attR2"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSSSPSTSLYKKAERET*"
     promoter        7613..7636
                     /label="lac_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSSSPSTSLYKKAERET*"
     gene            7697..8356
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSSSPSTSLYKKAERET*"
     CDS             7697..8356
                     /label="ORF frame 2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    8698..9003
                     /label="ccdB"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(9044..9151)
                     /label="attR1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    9044..9144
                     /label="attR2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     CDS             9171..498
                     /label="ORF frame 3"
                     /translation="MGGRSRGPKLTREFVDSRQMAVFIHNFKRKGGIGGYSAGERIVD
                     IIATDIQTKELQKQITKIQNFRVYYRDSRDPVWPRLEGVGVAPFPRQPWVCAGTRLLW
                     AWFRETQRRRPWVSHILHVRSQRHPDLRRYPCGPPGDASCSAPKSGRFLAVRGVPDVT
                     NGSRTSH*"
ORIGIN
    1 ggccgctcta gagggcccaa gcttacgcgt gaattcgtcg actcgagaca aatggcagta
   61 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
  121 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
  181 caaaattttc gggtttatta cagggacagc agagatccag tttggccgcg gctcgagggg
  241 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
  301 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
  361 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
  421 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
  481 agccgcacgt ctcactagtg gatcccccgg gctgcaggaa ttcgataatt ctgtggaatg
  541 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
  601 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
  661 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
  721 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
  781 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
  841 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
  901 ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
  961 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
 1021 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
 1081 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
 1141 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
 1201 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
 1261 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
 1321 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
 1381 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
 1441 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
 1501 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
 1561 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
 1621 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
 1681 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
 1741 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
 1801 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
 1861 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
 1921 actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc
 1981 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
 2041 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
 2101 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
 2161 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctcg
 2221 agggggggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
 2281 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
 2341 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
 2401 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
 2461 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
 2521 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
 2581 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
 2641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
 2701 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
 2761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
 2821 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
 2881 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
 2941 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
 3001 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
 3061 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
 3121 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
 3181 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
 3241 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
 3301 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
 3361 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
 3421 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
 3481 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
 3541 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
 3601 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
 3661 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
 3721 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
 3781 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
 3841 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
 3901 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
 3961 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
 4021 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
 4081 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
 4141 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
 4201 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
 4261 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
 4321 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
 4381 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
 4441 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
 4501 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
 4561 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
 4621 aggatctagg tgaagatcct ttttgataat ctcatgagga agcggaagag cgctgcgcgt
 4681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
 4741 agagctacca actctttttc cgaaaggtaa ctggcttcag cagagcgcag ataccaaata
 4801 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
 4861 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
 4921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
 4981 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
 5041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
 5101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
 5161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
 5221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
 5281 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
 5341 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
 5401 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
 5461 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
 5521 agcgcaacgc aattaatgtg agttagctca ctcggataac aatttcacac aggaaacagc
 5581 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
 5641 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
 5701 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
 5761 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
 5821 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
 5881 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
 5941 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
 6001 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
 6061 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
 6121 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
 6181 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
 6241 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
 6301 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
 6361 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
 6421 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
 6481 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
 6541 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
 6601 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
 6661 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
 6721 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
 6781 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
 6841 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
 6901 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
 6961 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
 7021 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
 7081 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
 7141 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
 7201 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
 7261 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
 7321 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
 7381 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
 7441 ggtatcgatc ctcgagccca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
 7501 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
 7561 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
 7621 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
 7681 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
 7741 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
 7801 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
 7861 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
 7921 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
 7981 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
 8041 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
 8101 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
 8161 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
 8221 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
 8281 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
 8341 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta
 8401 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
 8461 aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
 8521 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
 8581 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
 8641 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg
 8701 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
 8761 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
 8821 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
 8881 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
 8941 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
 9001 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
 9061 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
 9121 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atgggc

//