pPZP200

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pPZP200
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: none
载体大小: 6741 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签:
载体抗性: 壮观霉素或链霉素
筛选标记: none
克隆菌株: HB101
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 瞬表达
组成型/诱导型: --
病毒/非病毒: 非病毒

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载体质粒图谱和多克隆位点信息

pPZP200载体图谱



pPZP200 多克隆位点

载体简介

载体序列

LOCUS       pPZP200	6741 bp 	DNA	SYN
DEFINITION  pPZP200
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="pPZP200"
                     /mol_type="other DNA"
     CDS             202..888
                     /label="ORF frame 1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITG
                     TRRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWA
                     DTTTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHAR
                     ELIDQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAA
                     AENERQEEQA*"
     CDS             1187..1816
                     /label="ORF frame 2"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     CDS             complement(2091..2684)
                     /label="ORF frame 2"
                     /translation="MGNRHQYQVVIPHTGHAGRPCGNLYVPVWKLVADHLASSSVTLR
                     QTENGHVHDAATIAGAHVIEHRNEKIWLLVALGRLPNRRRTGCRRLPGFFADSISGRL
                     PRFTGACFCLDALPLGGLRGLQLLHQVITQRRADLYRAGWFATVTPIPRAWGFQCHCR
                     AGRQPSRLRLANRPFLHTWGIPRRRCLVVLDFPCRLL*"
     CDS             2211..3317
                     /label="ORF frame 3"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     misc_feature    complement(3813..3835)
                     /label="pGEX_3_primer"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     rep_origin      complement(4232..4851)
                     /label="pBR322_origin"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     gene            complement(5079..6078)
                     /label="spect"
                     /gene="spect"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(5079..6011)
                     /label="ORF frame 2"
                     /translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
                     LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
                     AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
ORIGIN
    1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
   61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
  121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
  181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
  241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
  301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
  361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
  421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
  481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
  541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
  601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
  661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
  721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
  781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
  841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
  901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
  961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
 1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
 1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
 1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
 1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
 1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
 1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
 1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
 1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
 1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
 1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
 1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
 1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
 1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
 1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
 1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
 1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
 1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
 2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
 2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
 2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
 2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
 2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
 2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
 2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
 2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
 2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
 2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
 2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
 2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
 2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
 2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
 2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
 2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
 3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
 3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
 3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
 3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
 3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
 3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
 3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
 3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
 3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
 3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
 3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
 3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
 3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
 3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
 3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
 3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
 3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
 4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
 4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
 4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
 4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
 4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
 4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
 4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
 4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
 4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
 4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
 4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
 4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
 4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
 4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
 4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
 4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
 4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
 5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
 5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
 5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
 5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
 5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
 5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
 5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
 5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
 5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
 5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
 5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
 5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
 5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
 5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
 5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
 5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
 6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
 6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
 6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
 6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
 6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
 6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
 6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
 6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
 6481 acgccgaatt gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
 6541 caagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
 6601 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg
 6661 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
 6721 cagggttccc ctcgggatca a
//

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