pCMV-VSVG

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pCMV-VSVG载体基本信息

出品公司: Cell Biolabs
载体名称: pCMV-VSVG
质粒类型: 逆转录病毒包装载体;封闭载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6363
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
备注:  
产品目录号: RV-110
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

pCMV-VSVG载体质粒图谱和多克隆位点信息

pCMV-VSVG 载体图谱



pCMV-VSVG载体简介

pCMV-VSVG载体序列

LOCUS       pCMV-VSVG	6363 bp 	DNA     circular	SYN
DEFINITION  pCMV-VSVG
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="pCMV-VSVG"
                     /mol_type="other DNA"
     promoter        84..660
                     /label="CMV_immearly_promoter"
     misc_feature    148..974
                     /label="5_LTR"
     misc_feature    163..450
                     /label="CAG_enhancer"
     misc_feature    617..637
                     /label="CMV_fwd_primer"
     promoter        618..687
                     /label="CMV_promoter"
     promoter        630..749
                     /label="CMV2_promoter"
     misc_feature    661..680
                     /label="pCEP_fwd_primer"
     misc_feature    663..687
                     /label="LNCX_primer"
     misc_feature    778..1350
                     /label="bGlob_int"
     CDS             1276..2955
                     /label="ORF frame 1"
     gene            1420..2955
                     /label="vsv-G"
                     /gene="vsv-G"
     misc_feature    2920..2952
                     /label="VSVG Tag"
     terminator      2974..3500
                     /label="rb_glob_PA_terminator"
     misc_feature    complement(3545..3567)
                     /label="M13_pUC_rev_primer"
     promoter        complement(3581..3610)
                     /label="lac_promoter"
     gene            complement(4693..5553)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4693..5553)
                     /label="ORF frame 1"
     promoter        complement(5595..5623)
                     /label="AmpR_promoter"
     misc_feature    complement(6140..6295)
                     /label="lacZ_a"
     misc_feature    6266..6288
                     /label="M13_pUC_fwd_primer"
     promoter        6281..6297
                     /label="M13_forward20_primer"
     promoter        6307..6325
                     /label="T7_promoter"
ORIGIN
    1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
   61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
  121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
  181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
  241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
  301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
  361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
  421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
  481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
  541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
  601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
  661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
  721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg
  781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
  841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
  901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
  961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
 1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
 1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
 1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
 1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
 1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
 1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
 1381 tctcatcatt ttggcaaaga attcctcgac ggatccgcca tgaagtgcct tttgtactta
 1441 gcctttttat tcattggggt gaattgcaag ttcaccatag tttttccaca caaccaaaaa
 1501 ggaaactgga aaaatgttcc ttctaattac cattattgcc cgtcaagctc agatttaaat
 1561 tggcataatg acttaatagg cacagcctta caagtcaaaa tgcccaagag tcacaaggct
 1621 attcaagcag acggttggat gtgtcatgct tccaaatggg tcactacttg tgatttccgc
 1681 tggtatggac cgaagtatat aacacattcc atccgatcct tcactccatc tgtagaacaa
 1741 tgcaaggaaa gcattgaaca aacgaaacaa ggaacttggc tgaatccagg cttccctcct
 1801 caaagttgtg gatatgcaac tgtgacggat gccgaagcag tgattgtcca ggtgactcct
 1861 caccatgtgc tggttgatga atacacagga gaatgggttg attcacagtt catcaacgga
 1921 aaatgcagca attacatatg ccccactgtc cataactcta caacctggca ttctgactat
 1981 aaggtcaaag ggctatgtga ttctaacctc atttccatgg acatcacctt cttctcagag
 2041 gacggagagc tatcatccct gggaaaggag ggcacagggt tcagaagtaa ctactttgct
 2101 tatgaaactg gaggcaaggc ctgcaaaatg caatactgca agcattgggg agtcagactc
 2161 ccatcaggtg tctggttcga gatggctgat aaggatctct ttgctgcagc cagattccct
 2221 gaatgcccag aagggtcaag tatctctgct ccatctcaga cctcagtgga tgtaagtcta
 2281 attcaggacg ttgagaggat cttggattat tccctctgcc aagaaacctg gagcaaaatc
 2341 agagcgggtc ttccaatctc tccagtggat ctcagctatc ttgctcctaa aaacccagga
 2401 accggtcctg ctttcaccat aatcaatggt accctaaaat actttgagac cagatacatc
 2461 agagtcgata ttgctgctcc aatcctctca agaatggtcg gaatgatcag tggaactacc
 2521 acagaaaggg aactgtggga tgactgggca ccatatgaag acgtggaaat tggacccaat
 2581 ggagttctga ggaccagttc aggatataag tttcctttat acatgattgg acatggtatg
 2641 ttggactccg atcttcatct tagctcaaag gctcaggtgt tcgaacatcc tcacattcaa
 2701 gacgctgctt cgcaacttcc tgatgatgag agtttatttt ttggtgatac tgggctatcc
 2761 aaaaatccaa tcgagcttgt agaaggttgg ttcagtagtt ggaaaagctc tattgcctct
 2821 tttttcttta tcatagggtt aatcattgga ctattcttgg ttctccgagt tggtatccat
 2881 ctttgcatta aattaaagca caccaagaaa agacagattt atacagacat agagatgaac
 2941 cgacttggaa agtgataagg atccgtcgag gaattcactc ctcaggtgca ggctgcctat
 3001 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
 3061 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
 3121 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
 3181 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
 3241 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
 3301 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
 3361 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
 3421 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
 3481 cctcttctct tatggagatc cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct
 3541 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
 3601 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
 3661 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
 3721 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
 3781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
 3841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
 3901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
 3961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
 4021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
 4081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
 4141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
 4201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
 4261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
 4321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
 4381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
 4441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
 4501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
 4561 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
 4621 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
 4681 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
 4741 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
 4801 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
 4861 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
 4921 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
 4981 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
 5041 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
 5101 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
 5161 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
 5221 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
 5281 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
 5341 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
 5401 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
 5461 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
 5521 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
 5581 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
 5641 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
 5701 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
 5761 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
 5821 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
 5881 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
 5941 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
 6001 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
 6061 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
 6121 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
 6181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
 6241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
 6301 cgcgcgtaat acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgctct
 6361 aga
//

pCMV-VSVG载体质粒图谱pdf版和相关资料下载

pCMV-VSVG载体应用举例

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