| 出品公司: | Microbix Biosystems |
|---|---|
| 载体名称: | pDC512 |
| 质粒类型: | 腺病毒表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | -- |
| 载体大小: | 3277 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | -- |
| 备注: | 克隆宿主菌：DB3.1 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
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载体基本信息
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载体简介
载体序列
LOCUS pDC512 3277 bp dsDNA Circular
DEFINITION Puc based frt shuttle plasmid for FLP-based Ad vector rescue Cummings & Graham, unpublished
COMMENT This file is best viewed with a free Vector NTI Viewer:
COMMENT http://www.informaxinc.com/view/viewer.html
COMMENT VNTREPLTYPE|Plasmid|
FEATURES Location/Qualifiers
rep_origin 967..1267
/label=ori
CDS complement(1717..2533)
/label=amp
misc_feature 2818..453
/label=Ad
misc_feature 509..542
/label=Frt
CDS 3187..1
/label=ITR
CDS complement(2..102)
/label=ITR
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gtcgacgagc tcaagcttag
481 atctgctagc aggcctgaat tcggatccga agttcctatt cttactagag tataggaact
541 tcgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
601 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
661 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
721 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccctgcat
781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
1021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
1081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
1141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
1201 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
1261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
1321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
1381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
1441 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
1501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
1561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
1621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1741 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
1801 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
1861 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
1921 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
1981 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
2041 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
2101 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
2161 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
2221 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
2281 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
2341 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
2401 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
2461 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
2521 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
2581 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
2641 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
2701 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
2761 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcactcta
2821 ggcaaaatag caccctcccg ctccagaaca acatacagcg cttcacagcg gcagcctaac
2881 agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga cacggcacca
2941 gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata taggactaaa
3001 aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc gaacctacgc
3061 ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg ttttcccacg
3121 ttacgtaact tcccatttta agaaaactac aattcccaac acatacaagt tactccgccc
3181 taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa ctccaccccc
3241 tcattatcat attggcttca atccaaaata aggtata
//
