pDONR Zeo

价格:800元

联系方式:I47-825O-882O

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pDONRZeo载体基本信息

出品公司: Invitrogen
载体名称: pDONR/Zeo, pDONRzeo, pDONR-Zeo
质粒类型: Gateway载体
高拷贝/低拷贝: 高拷贝
启动子:
克隆方法: Gateway; BP反应
载体大小: 4291bp
5' 测序引物及序列: M13F: TGTAAAACGACGGCCAGT
3' 测序引物及序列: M13R: CAGGAAACAGCTATGAC
载体标签:
载体抗性: 博来霉素、氯霉素
备注:
pDONR/zeo载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增;

构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
 
产品目录号: 12535-035
病毒/非病毒: 非病毒

pDONRZeo载体质粒图谱和多克隆位点信息

pDONR Zeo载体图谱



pDONR Zeo载体特征

pDONRZeo载体简介

pDONRZeo载体序列

LOCUS       pDONR\Zeo	4291 bp 	DNA	SYN
DEFINITION  pDONR\Zeo
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="pDONR/Zeo"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
     promoter        537..553
                     /label="M13_forward20_primer"
     misc_feature    complement(569..652)
                     /label="attL1"
     misc_feature    570..801
                     /label="attP"
     misc_feature    656..768
                     /label="attR1"
     misc_feature    complement(668..768)
                     /label="attR2"
     misc_feature    complement(1197..1502)
                     /label="ccdB"
     gene            complement(1847..2506)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             complement(1847..2506)
                     /label="ORF frame 1"
     misc_feature    2787..2887
                     /label="attR2"
     promoter        complement(3004..3022)
                     /label="T7_promoter"
     promoter        complement(3023..3041)
                     /label="M13_reverse_primer"
     misc_feature    complement(3111..3485)
                     /label="sh_ble"
     gene            complement(3114..3485)
                     /label="bleo"
                     /gene="bleo"
     promoter        complement(3486..3553)
                     /label="EM7_promoter"
     rep_origin      3625..4244
                     /label="pBR322_origin"
ORIGIN
    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
   61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
  121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
  181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
  241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
  301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
  361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
  421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
  481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
  541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
  601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
  661 AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA TTTTGCATAA
  721 AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCACTATGAA TCAACTACTT
  781 AGATGGTATT AGTGACCTGT AGTCGACCGA CAGCCTTCCA AATGTTCTTC GGGTGATGCT
  841 GCCAACTTAG TCGACCGACA GCCTTCCAAA TGTTCTTCTC AAACGGAATC GTCGTATCCA
  901 GCCTACTCGC TATTGTCCTC AATGCCGTAT TAAATCATAA AAAGAAATAA GAAAAAGAGG
  961 TGCGAGCCTC TTTTTTGTGT GACAAAATAA AAACATCTAC CTATTCATAT ACGCTAGTGT
 1021 CATAGTCCTG AAAATCATCT GCATCAAGAA CAATTTCACA ACTCTTATAC TTTTCTCTTA
 1081 CAAGTCGTTC GGCTTCATCT GGATTTTCAG CCTCTATACT TACTAAACGT GATAAAGTTT
 1141 CTGTAATTTC TACTGTATCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT
 1201 ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA
 1261 GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC
 1321 CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC GGGAGACTTT ATCTGACAGC
 1381 AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC
 1441 TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC
 1501 ATTTCACCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC
 1561 CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC
 1621 ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA TCATATATGC CTTGAGCAAC
 1681 TGATAGCTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG
 1741 ACATACTTCG GGTATACATA TCAGTATATA TTCTTATACC GCAAAAATCA GCGCGCAAAT
 1801 ACGCATACTG TTATCTGGCT TTTAGTAAGC CGGATCCACG CGGCGTTTAC GCCCCGCCCT
 1861 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC
 1921 AGACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT
 1981 ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA
 2041 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT
 2101 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA
 2161 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT
 2221 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG
 2281 CCATACGGAA TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT
 2341 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG
 2401 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC
 2461 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG
 2521 CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT
 2581 GAAAGTTGGA ACCTCTTACG TGCCGATCAA CGTCTCATTT TCGCCAAAAG TTGGCCCAGG
 2641 GCTTCCCGGT ATCAACAGGG ACACCAGGAT TTATTTATTC TGCGAAGTGA TCTTCCGTCA
 2701 CAGGTATTTA TTCGGCGCAA AGTGCGTCGG GTGATGCTGC CAACTTAGTC GACTACAGGT
 2761 CACTAATACC ATCTAAGTAG TTGATTCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT
 2821 ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG
 2881 TTTCTCGTTC AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT
 2941 GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA
 3001 TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT
 3061 CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA TCAGTCCTGC
 3121 TCCTCGGCCA CGAAGTGCAC GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC
 3181 CACGGCTGCT CGCCGATCTC GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC
 3241 ACGACCTCCG ACCACTCGGC GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG
 3301 GTGTTGTCCG GCACCACCTG GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG
 3361 ACCACACCGG CGAAGTCGTC CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG
 3421 AACTCGACCG CTCCGGCGAC GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG
 3481 GCCATGGTTT AGTTCCTCAC CTTGTCGTAT TATACTATGC CGATATACTA TGCCGATGAT
 3541 TAATTGTCAA CACGTGCTGA TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC
 3601 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC
 3661 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
 3721 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA
 3781 ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC
 3841 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT
 3901 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA
 3961 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
 4021 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC
 4081 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT
 4141 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT
 4201 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
 4261 TGGCCTTTTG CTGGCCTTTT GCTCACATGT T
//

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