| 质粒名称: | 8xGTIIC-luciferase |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 34615 |
| 存储实验室: | Stefano Piccolo |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3b |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 4800 bp |
| 载体修饰: | |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | synthetic TEAD luciferase reporter |
| 物种来源: | -- |
| 插入大小: | 360 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | 8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid # 34615 ; http://n2t.net/addgene:34615 ; RRID:Addgene_34615) |
| 参考文献部分: |
Role of YAP/TAZ in mechanotransduction. Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, Piccolo S. Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137. 10.1038/nature10137 PubMed 21654799
|
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 8xGTIIC-luciferase 5097 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION YAP/TAZ-responsive synthetic promoter driving luciferase expression.
ACCESSION .
VERSION .
KEYWORDS 8xGTIIC-luciferase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5097)
AUTHORS Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M,
Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N,
Piccolo S
TITLE Role of YAP/TAZ in mechanotransduction.
JOURNAL Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137.
PUBMED 21654799
REFERENCE 2 (bases 1 to 5097)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5097
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 238..359
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(275..294)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 329..348
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(400..2052)
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind 1968..1986
/label=LucNrev
/note="Firefly luciferase, reverse primer"
regulatory 2049..2058
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(2193..2209)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2194..2210)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_feature 2426..2517
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind complement(2458..2477)
/label=RVprimer3
/note="pGL3 vector, forward primer"
polyA_signal 2531..2579
/note="synthetic polyadenylation signal"
rep_origin complement(2710..3165)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2847..2868)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3059..3078
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3192..3296
/gene="bla"
/label=AmpR promoter
CDS 3297..4157
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3515..3534)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4328..4916
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4817..4836
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5070..5087
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
61 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
121 atcggtcgac ggatccttat cgattttacc acatttgtag aggttttact tgctttaaaa
181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
361 catgtctgct cgaagcggcc ggccgccccg actctagaat tacacggcga tctttccgcc
421 cttcttggcc tttatgagga tctctctgat ttttcttgcg tcgagttttc cggtaagacc
481 tttcggtact tcgtccacaa acacaactcc tccgcgcaac tttttcgcgg ttgttacttg
541 actggcgacg taatccacga tctctttttc cgtcatcgtc tttccgtgct ccaaaacaac
601 aacggcggcg ggaagttcac cggcgtcatc gtcgggaaga cctgcgacac ctgcgtcgaa
661 gatgttgggg tgttggagca agatggattc caattcagcg ggagccacct gatagccttt
721 gtacttaatc agagacttca ggcggtcaac gatgaagaag tgttcgtctt cgtcccagta
781 agctatgtct ccagaatgta gccatccatc cttgtcaatc aaggcgttgg tcgcttccgg
841 attgtttaca taaccggaca taatcatagg acctctcaca cacagttcgc ctctttgatt
901 aacgcccagc gttttcccgg tatccagatc cacaaccttc gcttcaaaaa atggaacaac
961 tttaccgacc gcgcccggtt tatcatcccc ctcgggtgta atcagaatag ctgatgtagt
1021 ctcagtgagc ccatatcctt gcctgatacc tggcagatgg aacctcttgg caaccgcttc
1081 cccgacttcc ttagagaggg gagcgccacc agaagcaatt tcgtgtaaat tagataaatc
1141 gtatttgtca atcagagtgc ttttggcgaa gaaggagaat agggttggca ccagcagcgc
1201 actttgaatc ttgtaatcct gaaggctcct cagaaacagc tcttcttcaa atctatacat
1261 taagacgact cgaaatccac atatcaaata tccgagtgta gtaaacattc caaaaccgtg
1321 atggaatgga acaacactta aaatcgcagt atccggaatg atttgattgc caaaaatagg
1381 atctctggca tgcgagaatc tcacgcaggc agttctatga ggcagagcga cacctttagg
1441 cagaccagta gatccagagg agttcatgat cagtgcaatt gtcttgtccc tatcgaagga
1501 ctctggcaca aaatcgtatt cattaaaacc gggaggtaga tgagatgtga cgaacgtgta
1561 catcgactga aatccctggt aatccgtttt agaatccatg ataataattt tttggatgat
1621 tgggagcttt ttttgcacgt tcaaaatttt ttgcaacccc tttttggaaa cgaacaccac
1681 ggtaggctgc gaaatgccca tactgttgag caattcacgt tcattataaa tgtcgttcgc
1741 gggcgcaact gcaactccga taaataacgc gcccaacacc ggcataaaga attgaagaga
1801 gttttcactg catacgacga ttctgtgatt tgtattcagc ccatatcgtt tcatagcttc
1861 tgccaaccga acggacattt cgaagtactc agcgtaagtg atgtccacct cgatatgtgc
1921 atctgtaaaa gcaattgttc caggaaccag ggcgtatctc ttcatagcct tatgcagttg
1981 ctctccagcg gttccatctt ccagcggata gaatggcgcc gggcctttct ttatgttttt
2041 ggcgtcttcc atggtggctt taccaacagt accggaatgc caagctcttg ggggatcgtc
2101 ccacggagcg gtgggtgccg gcggctgtct gggaagggct ccttgggggg cagaggcttt
2161 aaggtccccc cggcgcccac ccccaagctt atcgataccg tcgacctcga cctgcagatc
2221 tctagaagct tgcagtggaa tgtgtggaat gtgtctcaag cttgcagtgg aatgtgtgga
2281 atgtgtctca acttggcact ggccgggcta gaagcttgca gtggaatgtg tggaatgtgt
2341 ctcaagcttg cagtggaatg tgtggaatgt gtctcaactt ggcactggcc gggctagcac
2401 gcgtaagagc tcggtaccta tcgatagaga aatgttctgg cacctgcact tgcactgggg
2461 acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt
2521 actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
2581 cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt agcttgggct
2641 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
2701 gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
2761 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
2821 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
2881 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
2941 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
3001 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
3061 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
3121 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
3181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
3241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
3301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
3361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
3421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
3481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
3541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
3601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
3661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
3721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
3781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
3841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
3901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
3961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
4021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
4081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
4141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
4201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
4261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
4321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
4381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
4441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
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