pIB/V5-His-DEST

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价格：元

联系方式：I47-825O-882O

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pIB/V5-His-DEST载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pIB/V5-His-DEST
质粒类型:	Gateway载体；昆虫细胞表达
高拷贝/低拷贝:	高拷贝
启动子:	OplE2
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5038bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	His Tag (6x)
载体抗性:	氨苄青霉素
筛选标记:	Blasticidin
备注:	--
产品目录号:	12550-018
稳定性:	--
组成型:	组成型
病毒/非病毒:	杆状病毒

pIB/V5-His-DEST质粒图谱载体图谱和pIB/V5-His-DEST载体序列质粒序列多克隆位点信息

pIB-V5-His-DEST载体图谱

pIB/V5-His-DEST质粒载体简介

The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway entry clone and subsequent expression in Sf9, Sf21, or High Five insect cells. As part of the InsectSelect System, pIB/V5-His-DEST features:

 The OpIE2 promoter for constitutive expression
 Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site
 C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a nickel-chelating resin

pIB/V5-His-DEST质粒序列载体序列

CATGATGATAAACAATGTATGGTGCTAATGTTGCTTCAACAACAATTCTGTTGAACTGTGTTTTCATGTT
TGCCAACAAGCACCTTTATACTCGGTGGCCTCCCCACCACCAACTTTTTTGCACTGCAAAAAAACACGCT
TTTGCACGCGGGCCCATACATAGTACAAACTCTACGTTTCGTAGACTATTTTACATAAATAGTCTACACC
GTTGTATACGCTCCAAATACACTACCACACATTGAACCTTTTTGCAGTGCAAAAAAGTACGTGTCGGCAG
TCACGTAGGCCGGCCTTATCGGGTCGCGTCCTGTCACGTACGAATCACATTATCGGACCGGACGAGTGTT
GTCTTATCGTGACAGGACGCCAGCTTCCTGTGTTGCTAACCGCAGCCGGACGCAACTCCTTATCGGAACA
GGACGCGCCTCCATATCAGCCGCGCGTTATCTCATGCACGTGACCGGACACGAGGCGCCCGTCCCGCTTA
TCGCGCCTATAAATACAGCCCGCAACGATCTGGTAAACACAGTTGAACAGCATCTGTTCGAATTTAAAGC
TTGATATCGAATTCCTGCAGCCCAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAACGAGA
AACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGT
AAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGG
CTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAG
AAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTC
AGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAA
GAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAA
TTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCC
ATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACAT
ATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATG
TTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACT
TCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGAT
TCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGC
GATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATT
TGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTAT
GCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGT
CAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAA
GCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGA
ACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGG
ATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCT
GTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACC
ACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATG
ACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTC
TGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAAT
CTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATC
GACCCGGGTCTAGAGGGCCCGCGGTTCGAAGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA
CGCGTACCGGTCATCATCACCATCACCATTGAGTTTATCTGACTAAATCTTAGTTTGTATTGTCATGTTT
TAATACAATATGTTATGTTTAAATATGTTTTTAATAAATTTTATAAAATAATTTCAACTTTTATTGTAAC
AACATTGTCCATTTACACACTCCTTTCAAGCGCGTGGGATCGATGCTCACTCAAAGGCGGTAATACGGTT
ATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGT
AAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGT
GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCG
CTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAG
ACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCT
ACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGG
TGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTT
TCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGCCCTTGTTCCGAA
GGGTTGTGTCACGTAGGCCAGATAACGGTCGGGTATATAAGATGCCTCAATGCTACTAGTAAATCAGTCA
CACCAAGGCTTCAATAAGGAACACACAAGCAAGCCCTTTGAGTCAAGGGCTGCCGGGCTGCAGCACGTGT
TGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCC
AAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCT
CTGAAGACTACAGCGTCGCCGGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATA
TCATTTTACTGGGGGACCTTGCGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAAC
CTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGG
TTCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGG
GATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAAGCACTTCGTGGCCGAGGAGCAGGACTG
ACACGTCCCGGGAGATCTGCATGTCTACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTAT
TACCCATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTG
CACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACG
TTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGC
ATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC
CAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA
TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCA
ACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAG
ATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAG
ACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATA
CTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCT

pCEP4-DsRed2	pcDNA3.1+N-DYK-P2A
pAAVLINK-EF1a-HA-5-RALGAPB	FUW-tetO-hOCT4
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG	pHisB-Sumo-NeMeHg
pHBT-GFP-NOS	YEp620
pCold-SUMO	pAAV-EFS-DIO-GRAB_OT1.0
pRetroX-Tight-Hyg	pCEP4-Flag-N
pET28a-mOrange(M163A)	pLC4-14
pSEAP2-Basic	pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG
pYC25	pENTR U6
pBR327	pUTK21
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG	pHIL-D2
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG	pENTR5'/CMVp
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG	pLenti6/V5-GW/lacZ
pAMβ1	pSuper.Retro.Neo
pSwitch	pMCS-Green-Renilla-Luc
pLVX-AmCyan1-N1	pUC19
pQE-16	pAAVLINK-EF1a-HA-5'-ITSN1
pFastBac1-His-C	pFR-Luc
pDB262	pSW510
pGFP22	pCRII-TOPO/22-1
pBad33	pMAL-p5x
pGEX-6p-1-hsG3BP2b	pGEX-6p-1-AT5G48650
pBBR1MCS2-Tac-mCherry	YEp24
YEp16	Pgex-HRV3C
pcDNA4/TO/Myc-His/LacZ	pAAVLINK-EF1a-5'-PXDN
pIEX-3	pET-53-DEST
pFN10A (ACT)	pBABE-HAII
pIRESpuro3	pRI101-AN
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG	pcDNA3.1/Zeo(-)
pPM408	pDNAPolbeta_TA
pENTR-L1pLaclacZaL4	pVK101
pYES2-EGFP	pHOOK1
pAAV-hSyn-Cepheid1s-WPRE	pGL4.21[luc2P/Puro]
pCDEF-HA-Ub-K63R	pBTB-5
pAX01-ComK	pIZT/V5-His
pGL4.18[luc2P/Neo]	pAP1(PMA)-TA-Luc
p1JI008	pAAV-TRE-CAG-Fluc
pL-brevisGH1-TA	pBARMTE1
pCRE-DD-AmCyan1	pAAV-CAG-NES-YTnC
p19D+	pNov.aro-bgl1-640
pHY300PLK	pS-hydro_TA
pcDNA3.1-mRFP	pEYFP-Mem
pIEX-1	pDEST8
pMMB66EH	pQLinkN
pWY113 pET28a_AtmtE2	pShuttle-CMV
pIEx/Bac-3	pHT01
pHR31	pDM4
pcDNA3.1-CNTRFv1-HA-His	pcDNA6.2/nTC-Tag-DEST
pBluescript II KS(-)	pSP65-F1+
ppyrG	pAAVLINK-EF1a-HA-5'-DIP2C

pAKTaq	pCDH-DCX-TurboID-V5-puro
BCR/ABL P190 transgenic construct	pHIV-Luciferase
pCS2+HyPer7-NLS	pQCXIP-GFP-LacR
pET28GST-LIC	pINDUCER21-puro_Gateway-3xFLAG
shYAP1	PA-GFPmt
pAAV2/9n	SIRT6 Flag
pKO (Hyg)	pQCasTns(Vch)-entry(BsaI)
CD109-bio-His	pBLO 64.1
pEF1a-Usp9x_CD-mCherry	pCutamp
pGEX6p-1-BCCP-PupE	pcDNA3.1(+) CircRNA Mini Vector
PX551	GW1-Peredox-mCherry-NLS
pMXs-hSOX2	pShuttle-CMV-GFP U6promoter
GFP-MAP1B	pAc-sgRNA-Cas9
pEBTet_NAD-Snifit-mito	GAG-CRErec
pEGFP-C1 F-tractin-EGFP	LeGO-iG
p6XHis-T7(P266L)	CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre
pET16B.Pfu	pBSK
pscAAV-CAG-GFP	pGP-CMV-Gcamp6m
pcDNA3.1_AktPH-mCherry	PB533_15F11-sfGFP
pLTR-RD114A	pHCMV_DUGV_GP
pcDNA3.1 SIRT6_H133Y	EGFP-LC3
pAAVS1-PC-GCaMP6f	Myc tag WT PTB
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3	pRK5-HA-Ubiquitin-K29R
phage UbiC G3BP1-SNAP	pCI-6.20
pDriver	pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
pJRH-1179 U6-reci Gag-Cas9 v2	pT7-SpCas9_sgRNA-site1 (RTW443)
pCDNA3 Flag p38 alpha	BB3cK_pGAP_23*_pTEF_Cas9
pcDNA3.1-TYMS	pT7-hMLH1dn for IVT
pQUAST>stop>mCD8-GFP	LentiGuide_DKO
pBIG1a	pTA-HUH
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-	Fuw-dCas9-Dnmt3a_IM
pRK5-HA-SH3BP4 W92A	pCMT-flp
pMR-A-3	pBiFC-VN155(I152L)
pX330_SpCas9_USP7 Exon 3 gRNA	pGHYB
pCI-neo.mCherry-NEDD4	pCITEp65
pLenti-MP2	pcDNA5-T/O-Ds1-mCherry
pCAG-FAST-p10	pNG9
CAMK2D gRNA (BRDN0001146112)	pCRISPR-cBEST
pcDNA3 Connexin43-GFP-APEX2	murine TCR OTI-2A.pMIG II
pPB-AMBRA1-3xFLAG	pRH2502
pXR002:EF1a-dCasRx-2A-EGFP	Double UP mNeon to mScarlet
pDawn-sfGFP	pCMV-mA1-BE3
pTM1_VP30_ZEBOV	pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
pLIC-SM-HA	pSI-Target-AID-NG
AdEasier-1 cells (strain)	pLminP_Luc2P_RE4
pcDNA3.1-ERBB4	pEG302 22aa SunTag NtDRMcd (no NLS) g4+g
MG1655 ΔendA ΔrecA (DE3)	pMIH-TOMM20-Halo
pKAR2-Br512	mAzurite-8xbiRhoBAST-24xMS2v6
MP6	pBK092
pCMV-HA-JMJD3	mARG1 cassette 3

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pIB/V5-His-DEST

买家导航

pIB/V5-His-DEST载体质粒基本信息

pIB/V5-His-DEST质粒图谱载体图谱和pIB/V5-His-DEST载体序列质粒序列多克隆位点信息

pIB/V5-His-DEST质粒载体简介

pIB/V5-His-DEST质粒序列载体序列

pIB/V5-His-DEST载体图谱质粒图谱pdf版和pIB/V5-His-DEST载体序列质粒序列等相关资料下载

pIB/V5-His-DEST质粒载体应用举例

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