pYC119

价格:1200元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品人: Irene Castano Navarro(2015)
载体名称: pYC119
质粒类型: 酿酒酵母载体;复制型载体;过表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: TEF1
载体大小: 5796 bp
5' 测序引物及序列: M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’
3' 测序引物及序列: KS primer 5’ CGAGgtcgacggtatcg 3’
载体标签: 3XFLAG tag (C-ter)
载体抗性: 氨苄青霉素
筛选标记: URA3
克隆菌株: DH10B感受态细胞 ,30°C
宿主细胞(系): 出芽酵母(酿酒酵母)、白色念珠菌
备注: --
产品目录号: #63916
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYC119载体图谱



pYC119多克隆位点

载体简介

载体序列

LOCUS       pYC119	5796 bp 	DNA	SYN
DEFINITION  pYC119
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="pYC119"
                     /mol_type="other DNA"
     promoter        668..696
                     /label="AmpR_promoter"
     gene            738..1598
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             738..1598
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      1753..2372
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2724..2746
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        2745..2763
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        2780..2799
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3218..3234
                     /label="pBluescriptSK_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3253..3264
                     /label="EK"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3313..3327
                     /label="EK"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(3372..3388)
                     /label="pBluescriptKS_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             3624..4208
                     /label="ORF frame 3"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(3802..3820)
                     /label="T7_promoter"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(3830..3846)
                     /label="M13_forward20_primer"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(3839..3861)
                     /label="M13_pUC_fwd_primer"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             complement(4577..5380)
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     gene            complement(4580..5380)
                     /label="URA3"
                     /gene="URA3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             4869..5345
                     /label="ORF frame 3"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
ORIGIN
    1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
   61 cttaGCAGGT TAGACTAACA TGCAAAAAGT TTATATTCAT TTTTGTAGTT ATTCTTGTTT
  121 TCATTAGGAT GATTACACAC TGTGGATGTG GATTAATCGA TCATTGTTAA TTTAGTGTAG
  181 CTACTACCAT TTCAAACAAA ACGTAAATTC TTGCAGTCGT ACTGGATCTG TGAATCTATT
  241 AGTATATATG AATTAAAGTA GCTTGTACAT ATTATTCTGT TGAATCATAT CGCAGAGCAT
  301 TGGTTGAAAT CCAAAATATA AAAATGTAAT ATCACAAAAA ATAATACTAA TTCTAACATT
  361 AATGGGTCAG ATTTTTAGTG AATACTTAAA TTTATAATAT CGTCTATTTA AGCTAGCAAA
  421 TGGAACAACA TTTAAAGTAA GAACATCATA TCTACATGAA AATGTATATT TCAATCTGAC
  481 TAATAACGCA GAGCACATCT TTCAGTGATG TCTGTCACAT GATCAAAAAG AATTGTATTT
  541 AAATAATTCA TAATAAAAGC TTAAAAAATT Acaataatga aaataaagtA AATAATGACA
  601 TGGGTAAGAG TTCCGAAATA GAATCTTAGT GTACAAAACA AAAATTGCAT CATTAGAGAT
  661 CCCCacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
  721 attgaaaaag gaagagtATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG
  781 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG
  841 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC
  901 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
  961 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
 1021 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA
 1081 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT
 1141 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG
 1201 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
 1261 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
 1321 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG
 1381 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG
 1441 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC
 1501 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
 1561 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAAct gtcagaccaa gtttactcat
 1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
 1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
 1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
 1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
 1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
 1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
 1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
 2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
 2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
 2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
 2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
 2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
 2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
 2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
 2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
 2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
 2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
 2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
 2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
 2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gAGCTCATAG
 2821 CTTCAAAATG TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC
 2881 CGTACCACTT CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG
 2941 GTGTCGTTAA TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT
 3001 TTTCTTCGTC GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT
 3061 TTTTTGATTT TTTTCTCTTT CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT
 3121 CAATTTCTCA AGTTTCAGTT TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC
 3181 TCATTAGAAA GAAAGCATAG CAATCTAATC TAAGTTTTCT AGaactagtg GATCCTAGGA
 3241 GCAGACTACA AAGATGACGA TGACAAAGGA GCCGATTATA AGGACGATGA CGATAAAGGG
 3301 GCTGACTACA AAGATGACGA TGACAAGTGA TAAgatcccc cgggctgcag gaattcgata
 3361 tcaagcttat cgataccgtc gacCTCGAGA ACACAACCCA CAGCTACCAC CATCAACAAT
 3421 ATTTATATAT ATAACGTACA CATAGAAATC ACACAAACAG AGTATTTATT CTTAACTACA
 3481 TGAACTACCA TCAGACCGTC TGGGCCACTA TATAATGTGC CATTCATAAA CGTGATCACT
 3541 TTACGTAGCA GGCAACCCCA GGTGAAAATT TTTCAGCGAG CTGCCAGATT GTCAGGTGAA
 3601 AAACTGAAAA AAACTTCTGG GCGATGAGCT TGTGGCGGGA AATTAAGTAT ATAAAGCAGT
 3661 TAGTTTCTTC TGCTTCTTGT GGTTCTGGGT ATTCTTGTAA TATTTGGAAG AATAGAAAAT
 3721 CAAGACTACT GCCTTTCTTT TCATATTATA GAGGAAGAAA TACGCACGAA CACGATATAG
 3781 AGGTAAAGGT ACCcaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
 3841 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
 3901 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
 3961 agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg
 4021 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
 4081 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
 4141 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
 4201 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
 4261 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
 4321 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
 4381 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
 4441 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg
 4501 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
 4561 cttataatac agttttTTAG TTTTGCTGGC CGCATCTTCT CAAATATGCT TCCCAGCCTG
 4621 CTTTTCTGTA ACGTTCACCC TCTACCTTAG CATCCCTTCC CTTTGCAAAT AGTCCTCTTC
 4681 CAACAATAAT AATGTCAGAT CCTGTAGAGA CCACATCATC CACGGTTCTA TACTGTTGAC
 4741 CCAATGCGTC TCCCTTGTCA TCTAAACCCA CACCGGGTGT CATAATCAAC CAATCGTAAC
 4801 CTTCATCTCT TCCACCCATG TCTCTTTGAG CAATAAAGCC GATAACAAAA TCTTTGTCGC
 4861 TCTTCGCAAT GTCAACAGTA CCCTTAGTAT ATTCTCCAGT AGATAGGGAG CCCTTGCATG
 4921 ACAATTCTGC TAACATCAAA AGGCCTCTAG GTTCCTTTGT TACTTCTTCT GCCGCCTGCT
 4981 TCAAACCGCT AACAATACCT GGGCCCACCA CACCGTGTGC ATTCGTAATG TCTGCCCATT
 5041 CTGCTATTCT GTATACACCC GCAGAGTACT GCAATTTGAC TGTATTACCA ATGTCAGCAA
 5101 ATTTTCTGTC TTCGAAGAGT AAAAAATTGT ACTTGGCGGA TAATGCCTTT AGCGGCTTAA
 5161 CTGTGCCCTC CATGGAAAAA TCAGTCAAGA TATCCACATG TGTTTTTAGT AAACAAATTT
 5221 TGGGACCTAA TGCTTCAACT AACTCCAGTA ATTCCTTGGT GGTACGAACA TCCAATGAAG
 5281 CACACAAGTT TGTTTGCTTT TCGTGCATGA TATTAAATAG CTTGGCAGCA ACAGGACTAG
 5341 GATGAGTAGC AGCACGTTCC TTATATGTAG CTTTCGACAT gatttatctt cgtttcctgc
 5401 aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac
 5461 tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt
 5521 cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa gaataaaaaa
 5581 aaaatgatga attgaattga aaagctgtgg tatggtgcac tctcagtaca atctgctctg
 5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
 5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agGtgcatgt
 5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//

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