| 质粒名称: | pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 101204 |
| 存储实验室: | Jennifer Doudna, Keith Joung |
|
载体骨架基本信息 |
|
| 载体名称: | pCT10 |
| 载体出品公司: | Doudna lab |
| 空载体大小: | -- |
| 完整质粒大小: | 9437 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368 |
| 物种来源: | Streptococcus pyogenes |
| 插入大小: | -- |
| 基因突变: | C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368 |
| GenBank编号 | -- |
| 启动子: | T7 |
| 标签/融合蛋白: | 10xHis（主干上的N端子）MBP（主干上的N终端）TEV站点（主干上的N终端） |
| 克隆方法: | Gibson Cloning |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 |
| 3’测序引物 | T7-term |
|
文献引用方法 |
|
| 材料和方法部分: | pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant was a gift from Jennifer Doudna & Keith Joung (Addgene plasmid # 101204 ; http://n2t.net/addgene:101204 ; RRID:Addgene_101204) |
| 参考文献部分: | Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature. 2017 Sep 20. doi: 10.1038/nature24268. 10.1038/nature24268 PubMed 28931002 |
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3
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pJSC057 - Bacterial expression plasmid for SpCas9∆REC3载体质粒基本信息
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3质粒图谱载体图谱和pJSC057 - Bacterial expression plasmid for SpCas9∆REC3载体序列质粒序列多克隆位点信息
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3质粒载体简介
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3质粒序列载体序列
LOCUS pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant 9437 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant.
ACCESSION .
VERSION .
KEYWORDS pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 F...
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9437)
AUTHORS Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington
LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
TITLE Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
JOURNAL Nature. 2017 Sep 20. doi: 10.1038/nature24268.
PUBMED 28931002
REFERENCE 2 (bases 1 to 9437)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9437
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 139..158
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 242..261
/label=T7
/note="T7 promoter, forward primer"
promoter 242..260
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 349..371
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 391..420
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 430..1530
/codon_start=1
/gene="malE (mutated)"
/product="maltose binding protein from E. coli"
/label=MBP
/note="This version of the gene does not encode a signal
sequence, so MBP will remain in the cytosol."
/translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
WYAVRTAVINAASGRQTVDEALKDAQT"
primer_bind 1504..1527
/label=MBP-F
/note="Maltose binding protein, forward primer"
CDS 1585..1605
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
primer_bind complement(5208..5226)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 5222..5269
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(5635..5663)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(5690..5708)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5776..5880
/gene="bla"
/label=AmpR promoter
CDS 5881..6741
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6099..6118)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6912..7500
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7401..7420
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7654..7671
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 7686..7825
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(7751..7770)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7911..7933
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(7927..8118)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
ORIGIN
1 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
61 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
121 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
181 gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa
241 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagtgccgg ctccggagag
301 ctctttaatt aagcggccgc cctgcaggac tcgagttcta gaaataattt tgtttaactt
361 taagaaggag atatacatat gaaatcttct caccatcacc atcaccatca ccatcaccat
421 ggttcttcta tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc
481 tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc
541 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc
601 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg
661 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc
721 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt
781 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat
841 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc
901 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac
961 gacattaaag acgtgggcgt ggataacgct ggcgcaaaag cgggtctgac cttcctggtt
1021 gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc
1081 tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac
1141 accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa
1201 ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca
1261 aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac
1321 aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt
1381 attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg
1441 tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact
1501 gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac
1561 aataacaaca acctcgggat cgaggaaaac ctgtacttcc aatccaatgc aatggataag
1621 aaatactcaa taggcttaga tatcggcaca aatagcgtcg gatgggcggt gatcactgat
1681 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc
1741 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgtg tgcgactcgt
1801 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttc ttatctacag
1861 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag
1921 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta
1981 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta
2041 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag
2101 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa
2161 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca
2221 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa
2281 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct
2341 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa
2401 ttacagcttt caaaagatac ttacgattgt gatttagata atttattggc gcaaattgga
2461 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca
2521 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa
2581 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt
2641 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt
2701 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg
2761 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg
2821 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg
2881 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc
2941 ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca
3001 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat
3061 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa acggtggaag tgtgtctgga
3121 caaggcgata gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa
3181 ggtattttac agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag
3241 ccagaaaata tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa
3301 aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt
3361 cttaaagagc atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat
3421 ctccaaaatg gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat
3481 tatgatgtcg atcacattgt tccacaaagt ttccttaaag acgattcaat agacaataag
3541 gtcttaacgc gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta
3601 gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt
3661 aagtttgata atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt
3721 tttatcaaac gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg
3781 gatagtcgca tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg
3841 attaccttaa aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta
3901 cgtgagatta acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact
3961 gctttgatta agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt
4021 tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa
4081 tatttctttt actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga
4141 gagattcgca aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat
4201 aaagggcgag attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc
4261 aagaaaacag aagtacagac aggcggattc tccaaggagt caattttacc aaaaagaaat
4321 tcggacaagc ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat
4381 agtccaacgg tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag
4441 aagttaaaat ccgttaaaga gttactaggg atcacaatta tggaaagaag ttcctttgaa
4501 aaaaatccga ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc
4561 attaaactac ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct
4621 agtgccggag aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt
4681 ttatatttag ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa
4741 caattgtttg tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa
4801 ttttctaagc gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac
4861 aaacatagag acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg
4921 acgaatcttg gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga
4981 tatacgtcta caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt
5041 tatgaaacac gcattgattt gagtcagcta ggaggtgact aataacattg gaagtggata
5101 acggatccgc gatcgcggcg cgccacctgg tggccggccg gtaccacgcg tgcgcgctga
5161 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
5221 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
5281 aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg
5341 ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga
5401 gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac
5461 tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca
5521 agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag
5581 atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa
5641 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
5701 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
5761 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
5821 tatccgctca tgagacaata accctgataa atgcttcaat aacattgaaa aaggaagagt
5881 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
5941 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
6001 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
6061 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
6121 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
6181 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
6241 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
6301 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
6361 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
6421 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
6481 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
6541 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
6601 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
6661 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
6721 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
6781 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
6841 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
6901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
6961 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
7021 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
7081 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
7141 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
7201 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
7261 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
7321 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
7381 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
7441 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
7501 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
7561 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
7621 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
7681 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
7741 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
7801 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
7861 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
7921 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
7981 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
8041 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
8101 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
8161 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
8221 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
8281 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
8341 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
8401 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
8461 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
8521 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
8581 ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga
8641 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
8701 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
8761 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
8821 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc
8881 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga
8941 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
9001 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
9061 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc
9121 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
9181 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg
9241 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc
9301 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag
9361 gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc
9421 agtagtaggt tgaggcc
//
