pBacPAK8-His

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clonetech, Biofeng
载体名称: pBacPAK8-His
质粒类型: 昆虫表达载体;杆状病毒表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Polyhedrin
载体大小: 5579 kb
5' 测序引物及序列: Bac1 Primer: AACCATCTCGCAAATAAATA
3' 测序引物及序列: Bac2 Primer: ACGCACAGAATCTAGCGCTT
载体标签: --
载体抗性: 氨苄青霉素
真核筛选标记:
克隆菌株: DH5α 等
宿主细胞(系): 果蝇细胞系 IPLB-Sf21
备注: 相关载体:pBacPAK8
产品目录号: BF593
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

pBacPAK8-His载体图谱

pBacPAK8-His多克隆位点

载体简介

载体序列

LOCUS       pBacPAK8-His            5579 bp    DNA     circular     24-JUL-2016
DEFINITION  pBacPAK8-His
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pBacPAK8-His|
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="pBacPAK8"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    1..1147
                     /vntifkey="21"
                     /label=Ac_5_flank
     promoter        1148..1248
                     /vntifkey="30"
                     /label=PH_promoter
     misc_feature    1174..1191
                     /vntifkey="21"
                     /label=polyhedrin_fwd_primer
     misc_feature    1378..2811
                     /vntifkey="21"
                     /label=Ac_3_flank
     rep_origin      2899..3205
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    3473..3495
                     /vntifkey="21"
                     /label=pGEX_3_primer
     promoter        3654..3682
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            3724..4584
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             3724..4584
                     /vntifkey="4"
                     /label=Amp\Resistance
     rep_origin      4739..5358
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    1259..1276
                     /vntifkey="21"
                     /label=His\Tag
BASE COUNT     1459 a      1217 c      1287 g      1616 t 
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag 
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa 
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt 
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt 
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca 
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa 
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg 
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt 
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt 
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag 
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct 
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat 
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt 
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt 
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg 
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt 
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta 
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta 
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc 
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata 
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata ccatgggtca 
     1261 tcatcatcac catcacggtg gtcgacaagc ttggatccct gcaggcctcg agttcgaatc 
     1321 tagaagatct ggtaccgagc tcgaattccc gggcggccgc ttaattaatt gatccgggtt 
     1381 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta 
     1441 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca 
     1501 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa 
     1561 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta 
     1621 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt 
     1681 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg 
     1741 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa 
     1801 ataaagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt 
     1861 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac 
     1921 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa 
     1981 ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt 
     2041 cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta 
     2101 ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg 
     2161 ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa 
     2221 atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt 
     2281 ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt 
     2341 gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg 
     2401 gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag 
     2461 aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga 
     2521 ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca 
     2581 gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta 
     2641 taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag 
     2701 caattgtatt gtaaagagat tgtctcaagc tcggatcgat cccgcacgcc gataacaagc 
     2761 cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gcctgatgcg 
     2821 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag 
     2881 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     2941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     3001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 
     3061 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg 
     3121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     3181 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta 
     3241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     3301 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc 
     3361 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 
     3421 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 
     3481 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 
     3541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 
     3601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     3661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     3721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     3781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     3841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     3901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     3961 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     4021 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     4081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     4141 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     4201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     4261 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     4321 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     4381 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     4441 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     4501 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     4561 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     4621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     4681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     4741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     4801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     4861 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 
     4921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     4981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     5041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     5101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     5161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     5221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     5281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     5341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     5401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     5461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     5521 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcag 
//

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