| 质粒名称: | pHR_Gal4UAS_IRES_mC_pGK_tBFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79123 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_Gal4UAS_IRES_mC_pGK_tBFP was a gift from Wendell Lim (Addgene plasmid # 79123 ; http://n2t.net/addgene:79123 ; RRID:Addgene_79123) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_Gal4UAS_IRES_mC_pGK_tBFP 11280 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentiviral vector for inducible expression of genes with Gal4DBDVP64
synNotch Receptors.
ACCESSION .
VERSION .
KEYWORDS pHR_Gal4UAS_IRES_mC_pGK_tBFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11280)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 11280)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11280
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 198..908
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(343..361)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 611..630
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(1028..1051)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 1413..1432
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 1501..2202
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
misc_feature 2238..2826
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2291..2311)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2709..2720)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(2829..2845)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2830..2846)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 2936..3169
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(3194..3212)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3195..3212)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3502..3521)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(3735..3754)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3854..3876
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3914..3932)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4000..4104
/gene="bla"
/label=AmpR promoter
CDS 4105..4965
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4323..4342)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5136..5724
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5625..5644
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5878..5895
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(5965..5985)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5970..6299
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6150..6285
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6212..6231
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 7433..7498
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 7628..7648
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 8073..8207
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8110..8129)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8164..8183
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 8375..9008
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 9055..9180
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 9677..9910
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 10424..10541
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 10767..10791
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 10886..179
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(11053..11070)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 11280..19
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
ORIGIN
1 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
61 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
121 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataaa
181 tgagcgctgg cgggtccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
241 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
301 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
361 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
421 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
481 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
541 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
601 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
661 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
721 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
781 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
841 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
901 acaagtaggg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc gcgactctag
961 agtcgacctg cacgaggtta acgaattcta ccgggtaggg gaggcgcttt tcccaaggca
1021 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
1081 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
1141 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
1201 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
1261 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
1321 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
1381 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
1441 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
1501 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
1561 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
1621 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
1681 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
1741 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
1801 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
1861 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
1921 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
1981 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
2041 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
2101 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
2161 tactgcgacc tccctagcaa actggggcac aagcttaatt aatgcaggca tgcaagcttg
2221 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
2281 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
2341 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
2401 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
2461 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
2521 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
2581 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
2641 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
2701 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
2761 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
2821 ccccgcatcg ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca
2881 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
2941 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
3001 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
3061 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
3121 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat
3181 taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
3241 attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
3301 tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
3361 gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
3421 gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg
3481 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
3541 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
3601 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
3661 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa
3721 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
3781 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
3841 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
3901 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
3961 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
4021 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
4081 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
4141 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
4201 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
4261 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
4321 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
4381 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
4441 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
4501 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
4561 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
4621 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
4681 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
4741 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
4801 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
4861 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
4921 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
4981 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
5041 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
5101 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
5161 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
5221 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
5281 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
5341 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
5401 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
5461 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
5521 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
5581 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
5641 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
5701 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
5761 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
5821 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
5881 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
5941 tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
6001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
6061 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
6121 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
6181 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
6241 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
6301 agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca
6361 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
6421 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
6481 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
6541 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
6601 gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg
6661 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
6721 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
6781 gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
6841 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
6901 ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
6961 ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt
7021 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
7081 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
7141 caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt
7201 aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca
7261 cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc
7321 taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt
7381 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
7441 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
7501 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
7561 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
7621 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
7681 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
7741 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
7801 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
7861 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
7921 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
7981 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
8041 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
8101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
8161 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
8221 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
8281 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
8341 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
8401 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
8461 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
8521 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
8581 caccctgtga gcctgcatgg gatggatgac ccgtagagag aagtgttaga gtggaggttt
8641 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
8701 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
8761 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
8821 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
8881 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
8941 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
9001 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
9061 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
9121 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
9181 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
9241 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
9301 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
9361 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
9421 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
9481 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
9541 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
9601 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
9661 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
9721 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
9781 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
9841 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
9901 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
9961 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
10021 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
10081 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
10141 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
10201 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
10261 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
10321 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
10381 acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg
10441 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
10501 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
10561 gcagagatcc agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac
10621 gtcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
10681 ccgaacggag catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agttaggcgt
10741 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
10801 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctctcgaca
10861 ttcgttggat ccttgacttg cggcccccct ctccctcccc cccccctaac gttactggcc
10921 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
10981 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
11041 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
11101 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
11161 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
11221 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
//
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