pCAMBIA0305.1

价格:1800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pCAMBIA0305.1
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lac
载体大小: 9662 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: GUS、6xHis
载体抗性: 卡那霉素
筛选标记: --
克隆菌株: HB101等菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCAMBIA0305.1载体图谱



pCAMBIA0305.1 多克隆位点

载体简介

载体序列

LOCUS       pCAMBIA0305.1           9662 bp ds-DNA     circular SYN 25-JUL-2016
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            kanamycin-resistance and GUSPlus(TM) genes.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Cambia
  TITLE     Direct Submission
  JOURNAL   Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector 
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 4740.
COMMENT     VNTDATE|658800000|
COMMENT     VNTDBDATE|776304000|
COMMENT     VNTNAME|pCAMBIA0305.1|
COMMENT     VNTAUTHORNAME|Cambia|
FEATURES             Location/Qualifiers
     CDS             join(2..16,207..2024)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
     intron          17..206
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2031..2048
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
     terminator      2080..2332
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2354..2378
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3678..4307
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             4736..5809
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5875..6069
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6413..6553
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6739..7327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7414..8208)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
     misc_feature    8633..8657
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        8761..8791
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8799..8815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8823..8839
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8835..9068
                     /codon_start=1
                     /gene="lacZ (truncated)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
     misc_feature    8849..8905
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8909..8925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9302..9647
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus
                     CaMV 35S promoter"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
      241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag
      301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
      361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt
      421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg
      481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg
      541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg
      601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
      661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
      721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
      781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
      841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
      901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac
      961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
     1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca
     1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc
     1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca
     1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg
     1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca
     1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg
     1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
     1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
     1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc
     1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc
     1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc
     1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg
     1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
     1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
     1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
     1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct
     1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc
     2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
     2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
     2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     4741 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     4801 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     4861 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     4921 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     4981 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     5041 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     5101 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     5161 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     5221 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     5281 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     5341 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     5401 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     5461 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     5521 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     5581 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     5641 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     5701 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     5761 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     5821 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     5881 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     5941 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     6001 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     6061 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     6121 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     6181 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     6241 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     6301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     6361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     6421 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     6481 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     6541 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7381 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     7441 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     7501 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     7561 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     7621 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     7681 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     7741 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     7801 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     7861 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     7921 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     7981 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     8041 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     8101 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     8161 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     8221 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     8281 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     8341 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     8401 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     8461 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     8521 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     8581 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     8641 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     8701 taatgtactg aattaacgcc gaattaatgt gagttagctc actcattagg caccccaggc
     8761 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8821 cacaggaaac agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag
     8881 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     8941 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     9001 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     9061 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttagcttca
     9121 tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt
     9181 tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc
     9241 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
     9301 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
     9361 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
     9421 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
     9481 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     9541 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     9601 aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcttg
     9661 ac
//

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