| 质粒名称: | 3XFlag-pA-Tn5-Fl |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 124601 |
| 存储实验室: | Steven Henikoff |
|
载体骨架基本信息 |
|
| 载体名称: | pTXB1-Tn5 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8079 bp |
| 完整质粒大小: | 8679 bp |
| 载体修饰: | 核糖体结合位点（RBS）被一个典型的RBS序列取代 |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Protein A and hyperactive Tn5 transposase (Tnp) fusion protein,3XFlag_pA-Tn5 |
| 物种来源: | E. coli |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | T7 |
| 标签/融合蛋白: | 蛋白A和Tn5转座酶融合蛋白，带有N端3XFlag标签（插入N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NcoI(未破坏) |
| 3' 克隆位点: | NdeI (未破坏) |
| 5' 测序引物 | CGG TTT AAA CCG GGG ATC TCG |
| 3' 测序引物 | TTG CGC CGC AAC ATT CAC C |
|
文献引用方法 |
|
| 材料和方法部分: | 3XFlag-pA-Tn5-Fl was a gift from Steven Henikoff (Addgene plasmid # 124601 ; http://n2t.net/addgene:124601 ; RRID:Addgene_124601) |
| 参考文献部分: | CUT&Tag for efficient epigenomic profiling of small samples and single cells. Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5. 10.1038/s41467-019-09982-5 PubMed 31036827 |
买家导航
3XFlag-pA-Tn5-Fl载体质粒基本信息
3XFlag-pA-Tn5-Fl质粒图谱载体图谱和3XFlag-pA-Tn5-Fl载体序列质粒序列多克隆位点信息
3XFlag-pA-Tn5-Fl质粒载体简介
3XFlag-pA-Tn5-Fl质粒序列载体序列
LOCUS 3XFlag-pA-Tn5-Fl 8679 bp ds-DNA circular SYN 23-JUL-2019
DEFINITION Expresses protein A and Tn5 Transposase fusion protein in bacterial
cells.
ACCESSION .
VERSION .
KEYWORDS 3XFlag-pA-Tn5-Fl
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8679)
AUTHORS Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG,
Ahmad K, Henikoff S
TITLE CUT
JOURNAL Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5.
PUBMED 31036827
REFERENCE 2 (bases 1 to 8679)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8679
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 126..148
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(142..333)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(890..1972)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 1934..1953
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(1973..2050)
/gene="lacI"
/label=lacI promoter
terminator 2203..2289
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2386..2472
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2569..2655
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2752..2838
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2935..3021
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 3156..3175
/label=T7
/note="T7 promoter, forward primer"
promoter 3156..3174
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 3175..3199
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 3214..3236
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 3249..3314
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 3840..5267
/codon_start=1
/product="transposase from the bacterial Tn5 transposon
(Reznikoff, 1993)"
/label=Tn5 transposase
/translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
KLDGFLAAKDLMAQGIKI"
CDS 5268..5861
/codon_start=1
/gene="Mycobacterium xenopi gyrA"
/product="modified GyrA intein (Southworth et al., 1999)"
/label=Mxe GyrA intein
/note="N-terminal cleavage of this intein can be induced by
thiols."
/translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
CDS 5892..6047
/codon_start=1
/gene="Bacillus circulans chiA"
/product="chitin binding domain from chitinase A1 (Watanabe
et al., 1994)"
/label=CBD
/translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
PALWQLQ"
primer_bind complement(6112..6130)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 6126..6173
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 6233..6337
/gene="bla"
/label=AmpR promoter
CDS 6338..7198
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6556..6575)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin complement(7240..7753)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7435..7456)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7647..7666
/label=F1ori-R
/note="F1 origin, reverse primer"
rep_origin 7864..8452
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8353..8372
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8606..8623
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(8645..8664)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
61 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
121 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
181 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc
241 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
301 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
361 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
421 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
481 gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc
541 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
601 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
661 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
721 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
781 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
841 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
961 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
1021 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
1081 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
1141 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
1201 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
1261 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
1321 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
1381 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
1441 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
1501 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
1561 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
1621 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
1681 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
1741 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
1801 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
1861 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
1921 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
1981 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
2041 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
2101 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc
2161 gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct
2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
2341 gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag
2401 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
2461 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
2521 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga
2581 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
2641 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
2701 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa
2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
2821 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
2881 gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata
2941 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
3001 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
3061 ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt
3121 cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt
3181 gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata
3241 ccatgggtga ttacaaggat cacgatggcg attacaagga tcacgatatc gattacaagg
3301 atgatgatga taagatgacc atgattacgc caagcttaaa agatgaccca agccaaagtg
3361 ctaacctatt gtcagaagct aaaaagttaa atgaatctca agcaccgaaa gcggataaca
3421 aattcaacaa agaacaacaa aatgctttct atgaaatctt acatttacct aacttaaacg
3481 aagaacaacg caatggtttc atccaaagcc taaaagatga cccaagccaa agcgctaacc
3541 ttttagcaga agctaaaaag ctaaatgatg ctcaagcacc aaaagctgac aacaaattca
3601 acaaagaaca acaaaatgct ttctatgaaa ttttacattt acctaactta actgaagaac
3661 aacgtaacgg cttcatccaa agccttaaag acgatccttc agtgagcaaa gaaattttag
3721 cagaagctaa aaagctaaac gatgctcaag caccaaaaga tgacgataaa gaattcggtg
3781 gcggtggctc tggcggtggt gggagtggag gtgggggatc aggaggaggc ggttcccata
3841 tgattaccag tgcactgcat cgtgcggcgg attgggcgaa aagcgtgttt tctagtgctg
3901 cgctgggtga tccgcgtcgt accgcgcgtc tggtgaatgt tgcggcgcaa ctggccaaat
3961 atagcggcaa aagcattacc attagcagcg aaggcagcaa agccatgcag gaaggcgcgt
4021 atcgttttat tcgtaatccg aacgtgagcg cggaagcgat tcgtaaagcg ggtgccatgc
4081 agaccgtgaa actggcccag gaatttccgg aactgctggc aattgaagat accacctctc
4141 tgagctatcg tcatcaggtg gcggaagaac tgggcaaact gggtagcatt caggataaaa
4201 gccgtggttg gtgggtgcat agcgtgctgc tgctggaagc gaccaccttt cgtaccgtgg
4261 gcctgctgca tcaagaatgg tggatgcgtc cggatgatcc ggcggatgcg gatgaaaaag
4321 aaagcggcaa atggctggcc gctgctgcaa cttcgcgtct gagaatgggc agcatgatga
4381 gcaacgtgat tgcggtgtgc gatcgtgaag cggatattca tgcgtatctg caagataaac
4441 tggcccataa cgaacgtttt gtggtgcgta gcaaacatcc gcgtaaagat gtggaaagcg
4501 gcctgtatct gtatgatcac ctgaaaaacc agccggaact gggcggctat cagattagca
4561 ttccgcagaa aggcgtggtg gataaacgtg gcaaacgtaa aaaccgtccg gcgcgtaaag
4621 cgagcctgag cctgcgtagc ggccgtatta ccctgaaaca gggcaacatt accctgaacg
4681 cggtgctggc cgaagaaatt aatccgccga aaggcgaaac cccgctgaaa tggctgctgc
4741 tgaccagcga gccggtggaa agtctggccc aagcgctgcg tgtgattgat atttataccc
4801 atcgttggcg cattgaagaa tttcacaaag cgtggaaaac gggtgcgggt gcggaacgtc
4861 agcgtatgga agaaccggat aacctggaac gtatggtgag cattctgagc tttgtggcgg
4921 tgcgtctgct gcaactgcgt gaatctttta ctccgccgca agcactgcgt gcgcagggcc
4981 tgctgaaaga agcggaacac gttgaaagcc agagcgcgga aaccgtgctg accccggatg
5041 aatgccaact gctgggctat ctggataaag gcaaacgcaa acgcaaagaa aaagcgggca
5101 gcctgcaatg ggcgtatatg gcgattgcgc gtctgggcgg ctttatggat agcaaacgta
5161 ccggcattgc gagctggggt gcgctgtggg aaggttggga agcgctgcaa agcaaactgg
5221 atggctttct ggccgcgaaa gacctgatgg cgcagggcat taaaatctgc atcacgggag
5281 atgcactagt tgccctaccc gagggcgagt cggtacgcat cgccgacatc gtgccgggtg
5341 cgcggcccaa cagtgacaac gccatcgacc tgaaagtcct tgaccggcat ggcaatcccg
5401 tgctcgccga ccggctgttc cactccggcg agcatccggt gtacacggtg cgtacggtcg
5461 aaggtctgcg tgtgacgggc accgcgaacc acccgttgtt gtgtttggtc gacgtcgccg
5521 gggtgccgac cctgctgtgg aagctgatcg acgaaatcaa gccgggcgat tacgcggtga
5581 ttcaacgcag cgcattcagc gtcgactgtg caggttttgc ccgcgggaaa cccgaatttg
5641 cgcccacaac ctacacagtc ggcgtccctg gactggtgcg tttcttggaa gcacaccacc
5701 gagacccgga cgcccaagct atcgccgacg agctgaccga cgggcggttc tactacgcga
5761 aagtcgccag tgtcaccgac gccggcgtgc agccggtgta tagccttcgt gtcgacacgg
5821 cagaccacgc gtttatcacg aacgggttcg tcagccacgc tactggcctc accggtctga
5881 actcaggcct cacgacaaat cctggtgtat ccgcttggca ggtcaacaca gcttatactg
5941 cgggacaatt ggtcacatat aacggcaaga cgtataaatg tttgcagccc cacacctcct
6001 tggcaggatg ggaaccatcc aacgttcctg ccttgtggca gcttcaatga ctgcaggaag
6061 gggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
6121 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
6181 gaggaactat atccggataa ctacgtcagg tggcactttt cggggaaatg tgcgcggaac
6241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
6301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
6361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
6421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
6481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtttcc caatgatgag
6541 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
6601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
6661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
6721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
6781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
6841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
6901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
6961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
7021 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
7081 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
7141 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
7201 gtcagaccaa gtttactcat atatacttta gattgattta ccccggttga taatcagaaa
7261 agccccaaaa acaggaagat tgtataagca aatatttaaa ttgtaaacgt taatattttg
7321 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
7381 ggcaaaatcc cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt
7441 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
7501 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg
7561 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
7621 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
7681 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
7741 ctacagggcg cgtaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
7801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
7861 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
7921 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
7981 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
8041 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
8101 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
8161 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
8221 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
8281 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
8341 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
8401 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
8461 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
8521 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
8581 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcta tggtgcactc
8641 tcagtacaat ctgctctgat gccgcatagt taagccagt
//
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