| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141373 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 9165 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp7 |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141373 ; http://n2t.net/addgene:141373 ; RRID:Addgene_141373)
|
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro载体质粒基本信息
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro质粒图谱载体图谱和pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro载体序列质粒序列多克隆位点信息
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro质粒载体简介
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro质粒序列载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro 9165 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9165)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9165)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9165
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3820..3843
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 3880..3903
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 3915..4488
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4082..4099)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4309..4328
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4521..5120
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4521..4540)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5017..5037
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5134..5722
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5187..5207)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5605..5616)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5929..6562
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6690..6706)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6690..6706)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6703..6725)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6714..6730
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6738..6768)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6783..6804
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6921..6938)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7092..7680)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7172..7191)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7851..8711)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8474..8493
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8712..8816)
/gene="bla"
/label=AmpR promoter
polyA_signal 8864..8998
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8901..8920)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8955..8974
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tgagcaagat gagcgacgta aaatgcacgt cagttgttct cttgagcgtc
3601 ctgcaacaac tcagagtgga gtctagttcc aagctctggg ctcaatgcgt tcagcttcac
3661 aatgacattc tgttggcgaa agataccacg gaggcattcg agaagatggt ctcacttctg
3721 agcgttctcc tgtcaatgca aggggcggtc gatatcaata aattgtgtga ggaaatgctc
3781 gacaaccggg cgacattgca gctcgaaggc ggcgggggat ggagccatcc acaattcgag
3841 aaaggcggtg gttcaggagg aggtagcggg ggtggatcat ggtcacatcc gcagtttgaa
3901 aagtaaggat cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
3961 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
4021 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
4081 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
4141 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
4201 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
4261 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
4321 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
4381 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
4441 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
4501 ccacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
4561 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
4621 gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
4681 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
4741 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
4801 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
4861 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
4921 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
4981 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
5041 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
5101 cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac ctctggatta caaaatttgt
5161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
5221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
5281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
5341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
5401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
5461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
5521 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
5581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
5641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
5701 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
5761 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
5821 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
5881 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
5941 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
6001 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
6061 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
6121 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
6181 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
6241 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
6301 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
6361 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
6421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
6481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
6541 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
6601 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
6661 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
6721 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
6781 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
6841 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
6901 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
6961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
7021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
7081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
7141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
7201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
7261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
7321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
7381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
7441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
7501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
7561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
7621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
7681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
7741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
7801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
7861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
7921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
7981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
8041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
8101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
8161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
8221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
8281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
8341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
8401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
8461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
8521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
8581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
8641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
8701 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
8761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
8821 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
8881 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
8941 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
9001 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
9061 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
9121 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
