| 质粒名称: | pMito.iGABASnFR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112175 |
| 存储实验室: | Loren Looger |
|
载体骨架基本信息 |
|
| 载体名称: | pDisplay |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | iGABASnFR |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMito.iGABASnFR was a gift from Loren Looger (Addgene plasmid # 112175 ; http://n2t.net/addgene:112175 ; RRID:Addgene_112175) |
| 参考文献部分: | A genetically encoded fluorescent sensor for in vivo imaging of GABA. Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL. Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2. Epub 2019 Jul 15. 10.1038/s41592-019-0471-2 PubMed 31308547 |
买家导航
pMito.iGABASnF载体 pMitoiGABASnF质粒基本信息
pMito.iGABASnF质粒图谱 pMitoiGABASnF载体图谱和pMito.iGABASnF载体序列 pMitoiGABASnF质粒序列多克隆位点信息
pMito.iGABASnF质粒 pMitoiGABASnF载体简介
pMito.iGABASnF质粒序列 pMitoiGABASnF载体序列
LOCUS pMito.iGABASnFR 6810 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION GABA Sensor.
ACCESSION .
VERSION .
KEYWORDS pMito.iGABASnFR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6810)
AUTHORS Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP,
Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
Ahrens MB, Kullmann DM, Looger LL
TITLE A genetically encoded fluorescent sensor for in vivo imaging of
GABA.
JOURNAL Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
Epub 2019 Jul 15.
PUBMED 31308547
REFERENCE 2 (bases 1 to 6810)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6810
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 9..388
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 389..592
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 543..563
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 637..656
/label=T7
/note="T7 promoter, forward primer"
promoter 637..655
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 736..810
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
primer_bind 1887..1908
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(2011..2032)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2560..2577)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2566..2790
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(2922..3510)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3002..3021)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
polyA_signal 3839..3886
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind 3911..3930
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
CDS complement(4118..4912)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASKPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4229..4248)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 4839..4858
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind complement(5011..5030)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 5257..5277
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS complement(5360..6220)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5983..6002
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6221..6325)
/gene="bla"
/label=AmpR promoter
rep_origin 6352..6807
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6439..6458)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6649..6670
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
721 ttggggatat ccaccatgag cgtgctgacc cccctgctgc tgaggggcct gaccggcagc
781 gccaggaggc tgcccgtgcc cagggccaag atccacagcc tgggcgaccc ccccgtggcc
841 accagatctg aaagcatcaa tttcgtgagc tggggcggta gcacccagga tgcgcagaag
901 caggcctggg ccgacccgtt cagcaaggcc agcggcatta ccgtggtcca ggatgggccc
961 accgactacg gcaaactcaa ggccatggtc gaaagcggca acgtgcagtg ggacgtggtg
1021 gatgtagagg ccgacttcgc cttgcgcgcc gccgccgaag gcctgctcga acccctggat
1081 ttctcggtga tccagcgcga caagatcgac ccgcgcttcg tctccgacca tggcgtgggc
1141 tcgttcctgt tctctttcgt gctcggctac aacgaaggca agctcggagc cagcaagccc
1201 caggactgga ccgcgctgtt cgacaccaag acctaccccg gcaaacgcgc cctctacaaa
1261 tggccgagcc ctggcgtgct cgaactggcc ctgctcgccg acggcgtacc ggccgacaag
1321 ctctacccgc tggacctgga ccgcgccttc aagaaactcg acaccatcaa gaaagacatc
1381 gtctggtggg gcggcggtgc acagtcgcag cagctgctgg cctccggcga agtcagcatg
1441 ggccagttct ggaacggtcg catccacgcc ctgcaagaag acggcgcgcc agtgggcgtg
1501 agctggaagc agaacctggt gatggccgac atcctcgtcg tgcctaaagg cacgaagaac
1561 aaagccgccg cgatgaagtt cctggccagt gccagcagcg ccaaaggcca ggacgacttt
1621 tccgccctga ccgcctatgc tccagtgaac atcgacagtg tgcagcgcct cgaccttgcg
1681 caggtccgga tcaccgccga caagcagaag aacggcatca tggcgaactt caagatccgc
1741 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc
1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
1921 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat gagcaagggc
1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
2041 cacaagttca gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg
2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc
2281 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
2401 tggaacgcca acctggcacc gaacctgccg accgcctacg tcaaggatca gatcaccctc
2461 gatttcgcct actgggccaa gaacggtccg gccattgcga cacggtggaa tgaatggcta
2521 gtcaaactgc aggtcgacta ggcggccgct cgagatcagc ctcgactgtg ccttctagtt
2581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
2641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
2701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
2761 ggcgtgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agtggcggta
2821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
2881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
2941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
3001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
3061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
3121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
3181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
3241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
3301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
3361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
3421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
3481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
3541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
3601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
3661 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct
3721 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa
3781 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
3841 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
3901 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac
3961 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
4021 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
4081 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
4141 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
4201 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
4261 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
4321 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggct
4381 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
4441 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
4501 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
4561 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
4621 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
4681 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
4741 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
4801 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
4861 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
4921 atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct
4981 cactacttct ggaatagctc agaggccggg cctcggcctc tgcataaata aaaaaaatta
5041 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
5101 ttaggggcgg gactatggtt gctgactaat tgagatgctt gctttgcata cttctgcctg
5161 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc ttgctttgca
5221 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
5281 gctggttctt tccgcctcag gactcttcct ttttcaataa atcaatctaa agtatatatg
5341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
5401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
5461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
5521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
5581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
5641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
5701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
5761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
5821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
5881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
5941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
6001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
6061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
6121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
6181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
6241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
6301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
6361 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
6421 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
6481 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
6541 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
6601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
6661 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
6721 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
6781 ttaacaaaat attaacgctt acaatttacg
//
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