| 质粒名称: | pMRX-IP-GFP-LC3-RFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 84573 |
| 存储实验室: | Noboru Mizushima |
|
载体骨架基本信息 |
|
| 载体名称: | pMRX-IP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6100 bp |
| 完整质粒大小: | 7900 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | microtubule-associated protein 1 light chain 3 beta,Map1lc3b,LC3,LC3B |
| 物种来源: | R. norvegicus (rat) |
| 插入大小: | 1800 bp |
| 基因突变: | -- |
| GenBank编号 | U05784 NM_022867.2 |
| 启动子 | -- |
| 标签/融合蛋白: |
EGFP（插入的N端）
mRFP1（插入件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Bgl II（销毁时未知） |
| 3' 克隆位点: | Bgl II（销毁时未知） |
| 5' 测序引物 | CGACCACTACCAGCAGAACA |
| 3' 测序引物 | AAAAGACGGCAATATGGTGG |
|
文献引用方法 |
|
| 材料和方法部分: | pMRX-IP-GFP-LC3-RFP was a gift from Noboru Mizushima (Addgene plasmid # 84573 ; http://n2t.net/addgene:84573 ; RRID:Addgene_84573) |
| 参考文献部分: | An Autophagic Flux Probe that Releases an Internal Control. Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N. Mol Cell. 2016 Nov 17;64(4):835-849. doi: 10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3. 10.1016/j.molcel.2016.09.037 PubMed 27818143 |
买家导航
pMRX-IP-GFP-LC3-RFP载体质粒基本信息
pMRX-IP-GFP-LC3-RFP质粒图谱载体图谱和pMRX-IP-GFP-LC3-RFP载体序列质粒序列多克隆位点信息
pMRX-IP-GFP-LC3-RFP质粒载体简介
pMRX-IP-GFP-LC3-RFP质粒序列载体序列
LOCUS pMRX-IP-GFP-LC3-RFP 8546 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux.
ACCESSION .
VERSION .
KEYWORDS pMRX-IP-GFP-LC3-RFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8546)
AUTHORS Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y,
Kodama A, Ishihara T, Mizushima T, Mizushima N
TITLE An Autophagic Flux Probe that Releases an Internal Control.
JOURNAL Mol Cell. 2016 Nov 17;64(4):835-849. doi:
10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3.
PUBMED 27818143
REFERENCE 2 (bases 1 to 8546)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8546
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
LTR 734..1203
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 1268..1625
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1690..2106
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 2036..2058
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 2074..2090
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 2116..2490
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 2428..2444
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
regulatory 2505..2514
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2511..3227
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2556..2577)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2817..2836)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3164..3185
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3651..4328
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(3781..3799)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 4049..4068
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 4243..4266
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 4462..5048
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4629..4646)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4856..4875
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5049..5648
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5049..5068)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5545..5565
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5872..6341
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(6471..6488)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6642..7230)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6722..6741)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7394..8254)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8017..8036
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8255..8359)
/gene="bla"
/label=AmpR promoter
primer_bind 8427..8445
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg ctagagaacc atcagatgtt tccagggtgc cccaaggacc
421 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
481 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
541 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
601 ccgacttgtg gtctcgaaga ccccacctgt aggtttggca agctagctta agtaacgcca
661 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
721 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
781 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
841 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
901 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
961 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
1021 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgattgac
1081 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
1141 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
1201 tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc
1261 gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgactg
1321 attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg cggacccgtg
1381 gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc agggacttcg
1441 ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg actctttggt
1501 gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc
1561 ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc
1621 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
1681 atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
1741 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
1801 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
1861 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
1921 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
1981 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
2041 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
2101 ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc
2161 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct
2221 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg
2281 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac
2341 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc
2401 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc
2461 gaccccgggg gtggaccatc ctctagactg ccggatctcc cgccgccacc atggtgagca
2521 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
2581 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
2641 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
2701 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
2761 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
2821 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
2881 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
2941 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
3001 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
3061 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
3121 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
3181 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt ggcggtggct
3241 cgggcggtgg tgggtcgggt ggcggcggat ctatcgatga attgaattcc atgccgtccg
3301 agaagacctt caaacagcgc cggagcttcg aacaaagagt ggaagatgtc cggctcatcc
3361 gggagcagca ccccaccaag atcccagtga ttatagagcg atacaagggt gagaagcagc
3421 tgcccgtcct ggacaagacc aagttccttg tacctgatca cgtgaatatg agcgaactca
3481 tcaagataat tagaaggcgc ctgcagctca atgctaacca agccttcttc ctcctggtga
3541 atgggcacag catggtgagt gtgtccacac ccatctctga agtgtacgag agcgagagag
3601 atgaagacgg cttcctgtac atggtctatg cctcccagga gacgttcggg atggcctcct
3661 ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
3721 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
3781 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc
3841 agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
3901 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
3961 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
4021 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
4081 aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga
4141 ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca
4201 agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc
4261 acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg
4321 gcgcctaaag atcccagtgt ggtggtacgg gaattcctgc aggcctcgag ggccggcgcg
4381 ccgcggccgc tacgtaaatt ccgccccccc cccccccycc cccccccccc cccccccccc
4441 ccccmcmccc cccccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
4501 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
4561 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
4621 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
4681 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
4741 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
4801 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
4861 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
4921 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
4981 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
5041 ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
5101 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
5161 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
5221 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
5281 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
5341 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
5401 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
5461 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
5521 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
5581 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
5641 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
5701 gctcgacgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
5761 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
5821 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
5881 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
5941 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
6001 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
6061 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
6121 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
6181 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
6241 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
6301 ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa
6361 ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca ttaatgaatc
6421 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
6481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
6541 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
6601 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
6661 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
6721 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
6781 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
6841 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
6901 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
6961 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
7021 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
7081 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
7141 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
7201 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
7261 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
7321 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
7981 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
8401 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcact
8461 cgaggagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
8521 ctcatgtcca acattaccgc catgtt
//
