pLSC-5

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pLSC-5 
出品公司: 爱迪基因
目录编号: 62889
存储实验室: Feng Zhang

载体骨架基本信息
载体名称: lentiCRISPR
载体出品公司: Zhang lab
载体抗性: 氨苄霉素
空载体大小: 8809 bp
完整质粒大小: 13792 bp
载体类型: 哺乳细胞表达
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: SpCas9(574-1368)
物种来源: 光合细菌 Rhodopseudomonas palustris
插入大小: 2916 bp
基因突变: *see comment below
GenBank编号 JN247409
启动子 EFS
标签/融合蛋白: NLS SV40(N端)         FFBP12(N端)           P2A(C端)
克隆方法: 限制性内切酶
5' 克隆位点: Age1(未破坏)
3' 克隆位点: BamH1(未破坏)
5' 测序引物 NA
3' 测序引物  

文献引用方法
材料和方法部分: pLSC-5 was a gift from Feng Zhang (Addgene plasmid # 62889)
参考文献部分: A split-Cas9 architecture for inducible genome editing and transcription modulation. Zetsche B, Volz SE, Zhang F. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. 10.1038/nbt.3149 PubMed 25643054

载体图谱质粒图谱和多克隆位点信息

pLSC-5载体图谱

载体简介

载体序列

LOCUS       pLSC-5	13792 bp 	DNA	SYN
DEFINITION  pLSC-5
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="pLSC-5"
                     /mol_type="other DNA"
     promoter        239..815
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
     misc_feature    835..1015
                     /label="truncHIV-1_3_LTR"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     CDS             1558..2445
                     /label="ORF frame 1"
     misc_feature    1680..1913
                     /label="RRE"
     misc_feature    2444..2459
                     /label="cPPT"
     promoter        2617..2849
                     /label="hU6_promoter"
     misc_feature    2779..2798
                     /label="LKO1_5_primer"
     CDS             complement(2962..4452)
                     /label="ORF frame 2"
     CDS             3235..8868
                     /label="ORF frame 1"
     misc_feature    3241..3261
                     /label="SV40 NLS"
     misc_feature    3241..3264
                     /label="SV40 NLS"
     misc_feature    3247..3261
                     /label="NLS"
     gene            8281..8868
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    8886..9461
                     /label="WPRE"
     CDS             8974..9483
                     /label="ORF frame 1"
     CDS             8987..9517
                     /label="ORF frame 2"
     misc_feature    9527..9542
                     /label="cPPT"
     misc_feature    9527..9548
                     /label="U3PPT"
     misc_feature    9544..9596
                     /label="delta_U3"
     misc_feature    9597..9777
                     /label="truncHIV-1_3_LTR"
     misc_feature    9597..9777
                     /label="HIV-1_5_LTR"
     misc_feature    complement(9803..9820)
                     /label="BGH_rev_primer"
     terminator      9806..10033
                     /label="bGH_PA_terminator"
     rep_origin      10096..10402
                     /label="f1_origin"
     misc_feature    complement(10516..10536)
                     /label="pBABE_3_primer"
     misc_feature    complement(10522..10737)
                     /label="SV40_enhancer"
     promoter        10534..10802
                     /label="SV40_promoter"
     rep_origin      10701..10778
                     /label="SV40_origin"
     misc_feature    10763..10782
                     /label="SV40pro_F_primer"
     promoter        10896..10963
                     /label="EM7_promoter"
     misc_feature    10964..11338
                     /label="sh_ble"
     gene            10964..11335
                     /label="bleo"
                     /gene="bleo"
     terminator      11471..11590
                     /label="SV40_PA_terminator"
     misc_feature    11559..11578
                     /label="EBV_rev_primer"
     promoter        complement(11634..11652)
                     /label="M13_reverse_primer"
     misc_feature    complement(11651..11673)
                     /label="M13_pUC_rev_primer"
     promoter        complement(11687..11716)
                     /label="lac_promoter"
     gene            complement(12799..13659)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(12799..13659)
                     /label="ORF frame 2"
     promoter        complement(13701..13729)
                     /label="AmpR_promoter"
ORIGIN
    1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
   61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
  121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
  181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
  241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
  301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
  361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
  421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
  481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
  541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
  601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
  661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
  721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
  781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
  841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
  901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
  961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
 2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
 2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
 2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
 2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
 2821 ttggctttat atatcttGTG GAAAGGACGA AACACCgGAG ACGgaCGTCT Ctgttttaga
 2881 gctaGAAAta gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
 2941 gtcggtgcTT TTTTgaattc gctagctagg tcttgaaagg agtgggaatt ggctccggtg
 3001 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
 3061 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
 3121 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
 3181 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtgc caccATGGCC
 3241 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGGCGT GCAGGTGGAG
 3301 ACCATCAGCC CCGGCGACGG CAGAACCTTC CCCAAGAGAG GCCAGACCTG CGTGGTGCAC
 3361 TACACCGGCA TGCTGGAGGA CGGCAAGAAG TTCGACAGCA GCAGAGACAG AAACAAGCCC
 3421 TTCAAGTTCA TGCTGGGCAA GCAGGAGGTG ATCAGAGGCT GGGAGGAGGG CGTGGCCCAG
 3481 ATGAGCGTGG GCCAGAGAGC CAAGCTGACC ATCAGCCCCG ACTACGCCTA CGGCGCCACC
 3541 GGCCACCCCG GCATCATCCC TCCTCACGCC ACCCTGGTGT TCGACGTGGA GCTGCTGAAG
 3601 CTGGAGGGTG GCGGTGGCtc gGGTGGCGGT GGCtcgGGTG GCGGTGGCtc gTGCTTCGAC
 3661 TCCGTGGAAA TCTCCGGCGT GGAAGATCGG TTCAACGCCT CCCTGGGCGC ATACCACGAT
 3721 CTGCTGAAAA TTATCAAGGA CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG
 3781 GAAGATATCG TGCTGACCCT GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG
 3841 AAAACCTATG CCCACCTGTT CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC
 3901 ACCGGCTGGG GCAGGCTGAG CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC
 3961 AAGACAATCC TGGATTTCCT GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG
 4021 ATCCACGACG ACAGCCTGAC CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG
 4081 GGCGATAGCC TGCACGAGCA CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC
 4141 ATCCTGCAGA CAGTGAAGGT GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC
 4201 GAGAACATCG TGATCGAAAT GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC
 4261 AGCCGCGAGA GAATGAAGCG GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG
 4321 AAAGAACACC CCGTGGAAAA CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG
 4381 CAGAATGGGC GGGATATGTA CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC
 4441 GATGTGGACC ATATCGTGCC TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG
 4501 CTGACCAGAA GCGACAAGAA CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG
 4561 AAGAAGATGA AGAACTACTG GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG
 4621 TTCGACAATC TGACCAAGGC CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC
 4681 ATCAAGAGAC AGCTGGTGGA AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC
 4741 TCCCGGATGA ACACTAAGTA CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC
 4801 ACCCTGAAGT CCAAGCTGGT GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC
 4861 GAGATCAACA ACTACCACCA CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC
 4921 CTGATCAAAA AGTACCCTAA GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC
 4981 GACGTGCGGA AGATGATCGC CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC
 5041 TTCTTCTACA GCAACATCAT GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG
 5101 ATCCGGAAGC GGCCTCTGAT CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG
 5161 GGCCGGGATT TTGCCACCGT GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA
 5221 AAGACCGAGG TGCAGACAGG CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC
 5281 GATAAGCTGA TCGCCAGAAA GAAGGACTGG GACCCTAAGA AGTACGGCGG CTTCGACAGC
 5341 CCCACCGTGG CCTATTCTGT GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA
 5401 CTGAAGAGTG TGAAAGAGCT GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG
 5461 AATCCCATCG ACTTTCTGGA AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC
 5521 AAGCTGCCTA AGTACTCCCT GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT
 5581 GCCGGCGAAC TGCAGAAGGG AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG
 5641 TACCTGGCCA GCCACTATGA GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG
 5701 CTGTTTGTGG AACAGCACAA GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC
 5761 TCCAAGAGAG TGATCCTGGC CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG
 5821 CACCGGGATA AGCCCATCAG AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC
 5881 AATCTGGGAG CCCCTGCCGC CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC
 5941 ACCAGCACCA AAGAGGTGCT GGATGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC
 6001 GAGACACGGA TCGACCTGTC TCAGCTGGGA GGCGACAAAA GGCCGGCGGC CACGAAAAAG
 6061 GCCGGCCAGG CAAAAAAGAA AAAGGGATCC GGCGCAACAA ACTTCTCTCT GCTGAAACAA
 6121 GCCGGAGATG TCGAAGAGAA TCCTGGACCG CTAGATTTAG CTAGCTTAAT CCTCGACAAG
 6181 AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC
 6241 GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC
 6301 AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG
 6361 CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA
 6421 GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG
 6481 TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG
 6541 GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG
 6601 GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG
 6661 TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG
 6721 CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC
 6781 AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA
 6841 AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC
 6901 CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA
 6961 CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC
 7021 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC
 7081 GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG
 7141 AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG
 7201 CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT
 7261 GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG
 7321 GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTACG GAAGCAGCGG
 7381 ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG
 7441 CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC
 7501 CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC
 7561 TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC
 7621 AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGATGACCA ACTTCGATAA GAACCTGCCC
 7681 AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGTACGAGT ACTTCACCGT GTATAACGAG
 7741 CTGACCAAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG
 7801 CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG
 7861 CTGAAAGAGG ACTACTTCAA GAAAATCGAG GGTGGCGGTG GCtcgGGTGG CGGTGGCtcg
 7921 GGTGGCGGTG GCtcgGAGAT GTGGCACGAG GGCCTGGAGG AGGCCAGCAG ACTGTACTTC
 7981 GGCGAGAGAA ACGTGAAGGG CATGTTCGAG GTGCTGGAGC CCCTGCACGC CATGATGGAG
 8041 AGAGGCCCCC AGACCCTGAA GGAGACCAGC TTCAACCAGG CCTACGGCAG AGACCTGATG
 8101 GAGGCCCAGG AGTGGTGCAG AAAGTACATG AAGAGCGGCA ACGTGAAGGA CCTGACCCAG
 8161 GCCTGGGACC TGTACTACCA CGTGTTCAGA AGAATCAGCA AGCAGGGATC CGGCGCAACA
 8221 AACTTCTCTC TGCTGAAACA AGCCGGAGAT GTCGAAGAGA ATCCTGGACC GACCGAGTAC
 8281 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC
 8341 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG
 8401 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG
 8461 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG
 8521 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC
 8581 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC
 8641 CTGGCCACCG TCGGAGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG
 8701 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG
 8761 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG
 8821 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAC GCGTTAAGTC
 8881 GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT
 8941 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC
 9001 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG
 9061 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC
 9121 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC
 9181 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG
 9241 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG
 9301 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC
 9361 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT
 9421 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCGTCGACTT
 9481 TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG
 9541 GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATCT GCTTTTTGCT TGTACTGGGT
 9601 CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC
 9661 TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG
 9721 ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGgg
 9781 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
 9841 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
 9901 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
 9961 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
10021 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
10081 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
10141 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
10201 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
10261 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
10321 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
10381 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
10441 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
10501 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
10561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
10621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
10681 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
10741 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
10801 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
10861 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
10921 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
10981 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
11041 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
11101 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
11161 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
11221 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
11281 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
11341 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
11401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
11461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
11521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
11581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
11641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
11701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
11761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
11821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
11881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
11941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
12001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
12061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
12121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
12181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
12241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
12301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
12361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
12421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
12481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
12541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
12601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
12661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
12721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
12781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
12841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
12901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
12961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
13021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
13081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
13141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
13201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
13261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
13321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
13381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
13441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
13501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
13561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
13621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
13681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
13741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac

//

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