| 质粒名称: | pJMK004 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 34616 |
| 存储实验室: | Adam Cohen |
|
载体骨架基本信息 |
|
| 载体名称: | FCK(1.3) |
| 载体出品公司: | Pavel Osten |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 9227 bp |
| 完整质粒大小: | 10004 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Arch D95N |
| 物种来源: | Halorubrum sodomense |
| 插入大小: | 777 bp |
| 基因又名: | Archaerhodopsin 3 |
| 基因突变: | Changed Aspartic Acid 95 to Asparagine |
| 标签/融合蛋白: | GFP (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | GACGGATCCGCCACCATGGAC |
| 3' 测序引物 | CTAATCGAATTCTTAGTCGGCGGCAC |
|
文献引用方法 |
|
| 材料和方法部分: | pJMK004 was a gift from Adam Cohen (爱迪基因 plasmid # 34616) |
| 参考文献部分: | Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Kralj JM, Douglass AD, Hochbaum DR, Maclaurin D, Cohen AE. Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782. 10.1038/nmeth.1782 PubMed 22120467 |
买家导航
pJMK004载体质粒基本信息
pJMK004质粒图谱载体图谱和pJMK004载体序列质粒序列多克隆位点信息
pJMK004质粒载体简介
pJMK004质粒序列载体序列
LOCUS pJMK004 10745 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pJMK004
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10745)
AUTHORS Kralj JM, Douglass AD, Hochbaum DR, Maclaurin D, Cohen AE
TITLE Optical recording of action potentials in mammalian neurons using a
microbial rhodopsin.
JOURNAL Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782.
PUBMED 22120467
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pJMK004|
FEATURES Location/Qualifiers
source 1..10745
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
regulatory 3901..3910
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4696..5412
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(4738..4759)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4999..5018)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5346..5367
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5437..6025
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5490..5510)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5908..5919)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6028..6044)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6029..6045)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6550..6730
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6756..6773)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6762..6986
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7032..7460
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7119..7138)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7329..7350
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7469..7489)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7474..7803
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7654..7789
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7716..7735
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7851..7898
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7917..8291
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8421..8542
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8458..8477)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8512..8531
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8591..8607)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8591..8607)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8604..8626)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8615..8631
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8639..8669)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8684..8705
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8822..8839)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8993..9581)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9073..9092)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9752..10612)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10375..10394
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10613..10717)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
3541 tttgttcaca tccccttctc caaccccctc agtacatcac cctgggagaa caaggtccac
3601 ttgcttctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caacggagga
3661 gcaggttttg aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcaatcaagc tggttctcca
3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc
3901 gccaccatgg accccatcgc tctgcaggct ggttacgacc tgctgggtga cggcagacct
3961 gaaactctgt ggctgggcat cggcactctg ctgatgctga ttggaacctt ctactttctg
4021 gtccgcggat ggggagtcac cgataaggat gcccgggaat attacgctgt gactatcctg
4081 gtgcccggaa tcgcatccgc cgcatatctg tctatgttct ttggtatcgg gcttactgag
4141 gtgaccgtcg ggggcgaaat gttggatatc tattatgcca ggtacgccaa ctggctgttt
4201 accaccccac ttctgctgct ggatctggcc cttctcgcta aggtggatcg ggtgaccatc
4261 ggcaccctgg tgggtgtgga cgccctgatg atcgtcactg gcctcatcgg agccttgagc
4321 cacacggcca tagccagata cagttggtgg ttgttctcta caatttgcat gatagtggtg
4381 ctctattttc tggctacatc cctgcgatct gctgcaaagg agcggggccc cgaggtggca
4441 tctaccttta acaccctgac agctctggtc ttggtgctgt ggaccgctta ccctatcctg
4501 tggatcatag gcactgaggg cgctggcgtg gtgggcctgg gcatcgaaac tctgctgttt
4561 atggtgttgg acgtgactgc caaggtcggc tttggcttta tcctgttgag atcccgggct
4621 attctgggcg acaccgaggc accagaaccc agtgccggtg ccgatgtcag tgccgccgac
4681 cgaccggtag tagcagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
4741 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
4801 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
4861 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
4921 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
4981 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
5041 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
5101 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
5161 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
5221 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
5281 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
5341 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
5401 ctgtacaagt aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
5461 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
5521 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
5581 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
5641 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
5701 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
5761 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
5821 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
5881 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
5941 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
6001 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
6061 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
6121 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
6181 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
6241 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
6301 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
6361 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
6421 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
6481 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
6541 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
6601 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
6661 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
6721 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
6781 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
6841 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
6901 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
6961 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
7021 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
7081 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
7141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
7201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
7261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
7321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
7381 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
7441 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
7501 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
7561 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
7621 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
7681 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
7741 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
7801 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
7861 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
7921 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
7981 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
8041 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
8101 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
8161 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
8221 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
8281 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
8341 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
8401 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
8461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
8521 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
8581 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
8641 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
8701 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
8761 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
8821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
8881 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
8941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
9001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
9061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
9121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
9181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
9241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
9301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
9361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
9421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
9481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
9541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
9601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
9661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
9721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
9781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
9841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
9901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
9961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
10021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
10081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
10141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
10201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
10261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
10321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
10381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
10441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
10501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
10561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
10621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
10681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
10741 ctgac
//
