LOCUS pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag 13124 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION overexpression in human cells.
ACCESSION .
VERSION .
KEYWORDS pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13124)
AUTHORS Liang-Zhong Yang, Yang Wang, Si-Qi Li, Huang Wu, Gordon G.
Carmichael, Ling-Ling Chen
TITLE dCas13 imaging
JOURNAL Molecular Cell
REFERENCE 2 (bases 1 to 13124)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13124
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2223..3401
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2454..3392
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3349..3369
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3431..3451
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
regulatory 7010..7019
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 7742..7751
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 8237..8302
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
misc_feature 8309..8876
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(8470..8487)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8697..8716
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 8896..9495
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8896..8915)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 9392..9412
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 9514..10102
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(9567..9587)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(9985..9996)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 10177..10410
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(10529..10547)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 10615..10719
/gene="bla"
/label=AmpR promoter
CDS 10720..11580
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10938..10957)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 11751..12339
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 12240..12259
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 12493..12510
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 12602..12621
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 12750..12771
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 12786..12816
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 12824..12840
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 12829..12851
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 12848..12864
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 12848..12864
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 12914..12933
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
3421 agctagcatg cccaaaaaga agaggaaagt gaacatcccc gctctggtgg aaaaccagaa
3481 gaagtacttt ggcacctaca gcgtgatggc catgctgaac gctcagaccg tgctggacca
3541 catccagaag gtggccgata ttgagggcga gcagaacgag aacaacgaga atctgtggtt
3601 tcaccccgtg atgagccacc tgtacaacgc caagaacggc tacgacaagc agcccgagaa
3661 aaccatgttc atcatcgagc ggctgcagag ctacttccca ttcctgaaga tcatggccga
3721 gaaccagaga gagtacagca acggcaagta caagcagaac cgcgtggaag tgaacagcaa
3781 cgacatcttc gaggtgctga agcgcgcctt cggcgtgctg aagatgtaca gggacctgac
3841 caacgcctac aagacctacg aggaaaagct gaacgacggc tgcgagttcc tgaccagcac
3901 agagcaacct ctgagcggca tgatcaacaa ctactacaca gtggccctgc ggaacatgaa
3961 cgagagatac ggctacaaga cagaggacct ggccttcatc caggacaagc ggttcaagtt
4021 cgtgaaggac gcctacggca agaaaaagtc ccaagtgaat accggattct tcctgagcct
4081 gcaggactac aacggcgaca cacagaagaa gctgcacctg agcggagtgg gaatcgccct
4141 gctgatctgc ctgttcctgg acaagcagta catcaacatc tttctgagca ggctgcccat
4201 cttctccagc tacaatgccc agagcgagga acggcggatc atcatcagat ccttcggcat
4261 caacagcatc aagctgccca aggaccggat ccacagcgag aagtccaaca agagcgtggc
4321 catggatatg ctcaacgaag tgaagcggtg ccccgacgag ctgttcacaa cactgtctgc
4381 cgagaagcag tcccggttca gaatcatcag cgacgaccac aatgaagtgc tgatgaagcg
4441 gagcagcgac agattcgtgc ctctgctgct gcagtatatc gattacggca agctgttcga
4501 ccacatcagg ttccacgtga acatgggcaa gctgagatac ctgctgaagg ccgacaagac
4561 ctgcatcgac ggccagacca gagtcagagt gatcgagcag cccctgaacg gcttcggcag
4621 actggaagag gccgagacaa tgcggaagca agagaacggc accttcggca acagcggcat
4681 ccggatcaga gacttcgaga acatgaagcg ggacgacgcc aatcctgcca actatcccta
4741 catcgtggac acctacacac actacatcct ggaaaacaac aaggtcgaga tgtttatcaa
4801 cgacaaagag gacagcgccc cactgctgcc cgtgatcgag gatgatagat acgtggtcaa
4861 gacaatcccc agctgccgga tgagcaccct ggaaattcca gccatggcct tccacatgtt
4921 tctgttcggc agcaagaaaa ccgagaagct gatcgtggac gtgcacaacc ggtacaagag
4981 actgttccag gccatgcaga aagaagaagt gaccgccgag aatatcgcca gcttcggaat
5041 cgccgagagc gacctgcctc agaagatcct ggatctgatc agcggcaatg cccacggcaa
5101 ggatgtggac gccttcatca gactgaccgt ggacgacatg ctgaccgaca ccgagcggag
5161 aatcaagaga ttcaaggacg accggaagtc cattcggagc gccgacaaca agatgggaaa
5221 gagaggcttc aagcagatct ccacaggcaa gctggccgac ttcctggcca aggacatcgt
5281 gctgtttcag cccagcgtga acgatggcga gaacaagatc accggcctga actaccggat
5341 catgcagagc gccattgccg tgtacgatag cggcgacgat tacgaggcca agcagcagtt
5401 caagctgatg ttcgagaagg cccggctgat cggcaagggc acaacagagc ctcatccatt
5461 tctgtacaag gtgttcgccc gcagcatccc cgccaatgcc gtcgagttct acgagcgcta
5521 cctgatcgag cggaagttct acctgaccgg cctgtccaac gagatcaaga aaggcaacag
5581 agtggatgtg cccttcatcc ggcgggacca gaacaagtgg aaaacacccg ccatgaagac
5641 cctgggcaga atctacagcg aggatctgcc cgtggaactg cccagacaga tgttcgacaa
5701 tgagatcaag tcccacctga agtccctgcc acagatggaa ggcatcgact tcaacaatgc
5761 caacgtgacc tatctgatcg ccgagtacat gaagagagtg ctggacgacg acttccagac
5821 cttctaccag tggaaccgca actaccggta catggacatg cttaagggcg agtacgacag
5881 aaagggctcc ctgcagcact gcttcaccag cgtggaagag agagaaggcc tctggaaaga
5941 gcgggcctcc agaacagagc ggtacagaaa gcaggccagc aacaagatcc gcagcaaccg
6001 gcagatgaga aacgccagca gcgaagagat cgagacaatc ctggataagc ggctgagcaa
6061 cagccggaac gagtaccaga aaagcgagaa agtgatccgg cgctacagag tgcaggatgc
6121 cctgctgttt ctgctggcca aaaagaccct gaccgaactg gccgatttcg acggcgagag
6181 gttcaaactg aaagaaatca tgcccgacgc cgagaaggga atcctgagcg agatcatgcc
6241 catgagcttc accttcgaga aaggcggcaa gaagtacacc atcaccagcg agggcatgaa
6301 gctgaagaac tacggcgact tctttgtgct ggctagcgac aagaggatcg gcaacctgct
6361 ggaactcgtg ggcagcgaca tcgtgtccaa agaggatatc atggaagagt tcaacaaata
6421 cgaccagtgc aggcccgaga tcagctccat cgtgttcaac ctggaaaagt gggccttcga
6481 cacatacccc gagctgtctg ccagagtgga ccgggaagag aaggtggact tcaagagcat
6541 cctgaaaatc ctgctgaaca acaagaacat caacaaagag cagagcgaca tcctgcggaa
6601 gatccggaac gccttcgatg ccaacaatta ccccgacaaa ggcgtggtgg aaatcaaggc
6661 cctgcctgag atcgccatga gcatcaagaa ggcctttggg gagtacgcca tcatgaagga
6721 gggagctcta gaggggacta gtggaggtgg atccatggtg tccaagggcg aagaggacaa
6781 catggccagc ctgcctgcca cccacgagct gcacatcttc ggcagcatca acggcgtgga
6841 cttcgacatg gtgggacagg gcaccggcaa ccccaacgac ggctacgagg aactgaacct
6901 gaagtccaca aagggcgacc tgcagttcag cccctggatt ctggtgcccc acatcggcta
6961 cggcttccac cagtacctgc cctaccccga cggcatgagc ccttttcagg ccgccatggt
7021 ggatggcagc ggctaccagg tgcaccggac catgcagttt gaggacggcg ccagcctgac
7081 cgtgaactac cggtacacat acgagggcag ccacatcaag ggcgaggccc aagtgaaggg
7141 cacaggcttt ccagccgacg gccccgtgat gaccaatagc ctgacagccg ccgactggtg
7201 cagaagcaag aaaacctacc ccaatgacaa gaccatcatc agcaccttca agtggtccta
7261 caccaccggc aatggcaagc ggtacagaag caccgcccgg accacctaca ccttcgccaa
7321 acccatggcc gccaactacc tgaagaacca gcctatgtac gtgttccgca agaccgagct
7381 gaagcactcc aagacagaac tgaacttcaa agagtggcag aaagccttca ccgacgtgat
7441 gggcatggac gagctgtaca agtcgaattc tgcagtcgac ggtaccatgg tgtccaaggg
7501 cgaagaggac aacatggcca gcctgcctgc cacccacgag ctgcacatct tcggcagcat
7561 caacggcgtg gacttcgaca tggtgggaca gggcaccggc aaccccaacg acggctacga
7621 ggaactgaac ctgaagtcca caaagggcga cctgcagttc agcccctgga ttctggtgcc
7681 ccacatcggc tacggcttcc accagtacct gccctacccc gacggcatga gcccttttca
7741 ggccgccatg gtggatggca gcggctacca ggtgcaccgg accatgcagt ttgaggacgg
7801 cgccagcctg accgtgaact accggtacac atacgagggc agccacatca agggcgaggc
7861 ccaagtgaag ggcacaggct ttccagccga cggccccgtg atgaccaata gcctgacagc
7921 cgccgactgg tgcagaagca agaaaaccta ccccaatgac aagaccatca tcagcacctt
7981 caagtggtcc tacaccaccg gcaatggcaa gcggtacaga agcaccgccc ggaccaccta
8041 caccttcgcc aaacccatgg ccgccaacta cctgaagaac cagcctatgt acgtgttccg
8101 caagaccgag ctgaagcact ccaagacaga actgaacttc aaagagtggc agaaagcctt
8161 caccgacgtg atgggcatgg acgagctgta caagtccgga ctcagatctt gcctagagca
8221 acgcgtgcag ctaagcgact acaaagacca tgacggtgat tataaagatc atgacatcga
8281 ttacaaggat gacgatgaca agtaaggatc cctccccccc ccctaacgtt actggccgaa
8341 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
8401 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
8461 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
8521 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
8581 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
8641 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
8701 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
8761 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
8821 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataaggat
8881 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
8941 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
9001 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
9061 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
9121 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
9181 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
9241 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
9301 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
9361 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
9421 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
9481 aagcccggtg cctgacgccc atcgatagat cctaatcaac ctctggatta caaaatttgt
9541 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
9601 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
9661 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
9721 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
9781 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
9841 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
9901 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
9961 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
10021 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
10081 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
10141 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
10201 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
10261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
10321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
10381 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
10441 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
10501 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
10561 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
10621 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
10681 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
10741 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
10801 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
10861 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
10921 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
10981 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
11041 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
11101 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
11161 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
11221 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
11281 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
11341 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
11401 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
11461 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
11521 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
11581 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
11641 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
11701 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
11761 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
11821 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
11881 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
11941 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
12001 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
12061 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
12121 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
12181 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
12241 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
12301 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
12361 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
12421 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
12481 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
12541 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
12601 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
12661 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
12721 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
12781 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
12841 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
12901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
12961 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
13021 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
13081 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
