| 质粒名称: | pEGFP(C3)-Nup153 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64268 |
| 存储实验室: | Birthe Fahrenkrog |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C3 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4700bp |
| 完整质粒大小: | 9127 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | NuP153 / Nucleoporin 153kDa |
| 物种来源: | H. sapiens (human) |
| 启动子: | CMV |
| 标签/融合蛋白: | GFP (N terminal on backbone) |
| 克隆方法: | Restriction Enzyme |
| 5’克隆位点: | HindIII (not destroyed) |
| 3’克隆位点: | XmaI (not destroyed) |
| 5’测序引物 | EGFP-C |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: |
pEGFP(C3)-Nup153 was a gift from Birthe Fahrenkrog (Addgene plasmid # 64268 ; http://n2t.net/addgene:64268 ; RRID:Addgene_64268)
|
| 参考文献部分: | Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy. Duheron V, Chatel G, Sauder U, Oliveri V, Fahrenkrog B. Nucleus. 2014;5(6):601-12. doi: 10.4161/19491034.2014.990853. 10.4161/19491034.2014.990853 PubMed 25485891 |
买家导航
pEGFP(C3)-Nup153载体质粒基本信息
pEGFP(C3)-Nup153质粒图谱载体图谱和pEGFP(C3)-Nup153载体序列质粒序列多克隆位点信息
-Nup153质粒图谱.png)
pEGFP(C3)-Nup153质粒载体简介
pEGFP(C3)-Nup153质粒序列载体序列
LOCUS pEGFP(C3)-Nup153 9131 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION GFP-tagged mammalian expression of Nup153.
ACCESSION .
VERSION .
KEYWORDS pEGFP(C3)-Nup153
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9131)
AUTHORS Duheron V, Chatel G, Sauder U, Oliveri V, Fahrenkrog B
TITLE Structural characterization of altered nucleoporin Nup153 expression
in human cells by thin-section electron microscopy.
JOURNAL Nucleus. 2014;5(6):601-12. doi: 10.4161/19491034.2014.990853.
PUBMED 25485891
REFERENCE 2 (bases 1 to 9131)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9131
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 607..616
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 613..1329
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 613..1329
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(658..679)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(919..938)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1266..1287
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 5919..6040
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5956..5975)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6010..6029
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(6047..6502)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6184..6205)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6396..6415
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 6529..6633
/gene="bla"
/label=AmpR promoter
promoter 6635..6992
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(6658..6678)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 6843..6978
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6905..6924
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7027..7821
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7081..7100)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7691..7710
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(8009..8028)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 8053..8100
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 8429..9017
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8918..8937
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt actcagatct cgagctcaag cttatggcct cgggagccgg aggagtcgga
1381 gggggcggtg gcggcaagat ccggacgcgg cgttgccacc aggggccaat taagccttac
1441 cagcaggggc gacaacagca tcagggcatt cttagcaggg ttacagaatc tgttaagaat
1501 attgtgccag ggtggctaca aagatacttc aacaagaatg aagatgtatg cagctgttca
1561 acagacacaa gcgaggttcc acgctggcca gaaaataaag aggaccatct ggtatatgcc
1621 gatgaggaga gctctaatat tactgatggg agaatcacac ctgagccagc agtcagtaat
1681 acagaagaac cttcaacaac tagtactgct tcaaattatc cagatgtgtt aacaaggcct
1741 tctcttcatc ggagccatct gaatttttcc atgttggaat cccctgcatt acactgtcag
1801 ccatctacat cctcggcatt cccaattggc agttcgggat tttcccttgt aaaggaaatt
1861 aaagattcta cctctcagca tgatgatgat aacatctcaa ctaccagtgg tttttcttca
1921 agagcttctg ataaagatat aactgtttca aagaacactt cattgccacc tctgtggtcc
1981 ccagaagctg aacgttctca ctcactctca cagcacactg ccaccagctc aaaaaaacca
2041 gcattcaact tgtctgcctt tggaacactt tccccttcac ttgggaattc ttcaatcctt
2101 aaaaccagtc agcttggaga ttctcctttt tatcctggaa aaacaacgta cggtggggca
2161 gcagctgctg taagacagtc taaactacga aatacacctt atcaggcacc agttagaaga
2221 caaatgaaag ctaagcaact cagtgcacaa tcttacggtg tgaccagttc aacagctcgg
2281 cgaatattgc agtctttaga gaagatgtca agccctttag cggatgcaaa aagaattcca
2341 tccattgttt cttctcctct gaattctcct cttgatagga gtgggataga tatcacagat
2401 tttcaggcca aaagagaaaa ggtggattct caatatcctc ctgttcagag acttatgacc
2461 ccaaagccag tttccatagc aacaaatcga agtgtttatt ttaaaccatc tctgactcct
2521 tctggtgaat tcaggaagac taatcaaaga atagataaca agtgcagtac tggatatgaa
2581 aaaaatatga cacccggaca aaatagagaa caacgagaaa gtggcttttc atatccaaat
2641 ttcagtttgc ctgcagccaa tggtttatct tctggagtag gtggtggagg tggcaagatg
2701 agacgagaaa gaacacgctt tgttgcttct aaacctctgg aggaggagga aatggaagtt
2761 ccagtattac cgaaaatctc tctaccgatc accagttctt cactgcctac ctttaatttt
2821 agttcccctg agatcacaac ttcctctcca tcacccatca attcgtctca agcattaaca
2881 aacaaggtac aaatgacctc tccgagcagc actggcagtc ccatgtttaa attttcatct
2941 ccaatcgtaa aatctactga ggcaaatgta ctacctccat catctattgg atttacattt
3001 agtgtgcctg ttgcaaaaac agcagaactt tctggttcta gtagtacttt agaaccaatt
3061 ataagtagtt cagctcatca tgtcactaca gtgaacagta caaattgtaa gaagacacca
3121 cctgaagatt gtgagggtcc ttttagacct gcagaaatcc tgaaagaagg aagtgttcta
3181 gatattctga aaagccctgg tttcgcatcg ccgaagatag attctgttgc tgctcagccc
3241 accgcaacaa gcccagtagt ttatacaaga ccagcaataa gtagcttttc ttctagtgga
3301 attgggtttg gggagagttt aaaagctggg tcatcatggc agtgtgatac atgtctactc
3361 cagaacaaag ttacagacaa caaatgcata gcctgtcaag cagcaaaatt gtcacccaga
3421 gatactgcta aacagactgg aattgaaaca ccaaataaaa gtggcaaaac aactctttct
3481 gcatcaggga caggctttgg agacaaattt aaaccagtga taggcacttg ggattgtgat
3541 acctgtttag tgcaaaataa acctgaagca ataaaatgtg tagcctgtga aacaccgaaa
3601 cctggaactt gtgtgaagcg agcccttaca ttgacagtgg tttcggaaag tgctgagact
3661 atgactgctt catcttccag ctgcactgta accactggta ccttaggatt tggagataaa
3721 ttcaaaaggc ccattggatc ttgggagtgt tcagtatgct gtgtttctaa taatgcagaa
3781 gacaataagt gtgtgtcctg tatgtctgag aaaccaggaa gttcagtacc tacttcaagt
3841 agcagcactg tacctgtctc tctgccttct ggaggctctc taggattgga aaagttcaag
3901 aaacccgagg gaagctggga ctgtgaattg tgcctagtgc agaataaggc agactctacc
3961 aaatgtttgg catgtgaaag tgcaaagcca ggcacaaaat ctgggtttaa aggctttgac
4021 acatcttcct catcttcgaa ctcagcagcc tcctcatcct tcaaatttgg tgtctcatca
4081 tcctcttctg ggccttctca gactttaaca agcactggaa attttaaatt tggagatcag
4141 ggaggattca aaataggtgt gtcatctgat tctgggtcta taaaccccat gagtgaaggc
4201 tttaaatttt ctaaaccaat aggagatttt aaatttggag tttcatctga atctaagccc
4261 gaagaagtta aaaaagatag taagaatgat aattttaagt ttggactttc ttctggttta
4321 agcaacccag tttctttaac tccatttcaa tttggggtat ctaatcttgg acaggaagaa
4381 aagaaagagg aactgcccaa atcttcctct gcaggtttta gctttggtac aggtgttatt
4441 aactccaccc ctgctcctgc taacaccata gtgacctctg agaacaagag cagcttcaac
4501 cttggaacca tagaaaccaa gagtgcttca gtggctcctt tcacatgtaa gacatcagaa
4561 gctaaaaaag aagaaatgcc tgccaccaaa ggaggattct cttttggcaa cgtggagcct
4621 gcctctctgc catctgcctc agtgtttgtt ttgggaagga cagaagagaa acagcaagag
4681 cctgtcactt ctacttccct agtttttggg aagaaagctg acaatgaaga gccaaagtgt
4741 caaccagtgt tttcctttgg gaattcagag caaaccaaag atgagaattc ttcaaagtcc
4801 acatttagtt ttagtatgac aaaaccatct gagaaggaat ctgaacagcc agcaaaagcc
4861 acttttgcct ttggagctca aactagtact acagctgatc aaggtgcagc aaagccagtt
4921 tttagtttct tgaacaacag ttcctctagt tcaagtacac cagccacttc tgctggtggt
4981 ggcatatttg gtagttccac ctcttcctcc aatccacctg tggctacctt tgtgtttgga
5041 cagtccagca atcctgtgag cagctctgcc tttggtaaca ctgctgaatc cagcacctct
5101 cagtctttgc tattttctca agatagcaaa ctagcaacca catccagcac aggtacagct
5161 gtcaccccat ttgtctttgg tccaggagcc agcagtaata atactaccac ctctggtttc
5221 ggctttggag ccacaaccac atctagctct gcaggatcct cctttgtatt tggaactgga
5281 ccctcagcac catctgccag tccagcattt ggtgctaacc agaccccaac atttggacaa
5341 agtcaaggtg ccagccagcc caatccccca ggctttggat ctatatcatc ttccacagca
5401 ttatttccca ctggttctca gcctgcacca cctacttttg ggacagtgtc aagcagtagc
5461 cagccccctg tgtttggaca gcaacctagt cagtctgcat ttggctctgg aacaactcct
5521 aattctagtt cggctttcca gtttggcagc agcactacaa atttcaactt cacaaacaac
5581 agtccatcag gagtgttcac atttggtgca aattctagca cacctgcagc ctcagcccag
5641 ccttcaggct cggggggctt tccatttaac cagtctccag cagcatttac agtggggtca
5701 aatgggaaaa atgtgttctc ttcttctgga acttcattct ctggtcgcaa gataaagact
5761 gctgttagac gcaggaaatt atccccccgg gatccaccgg atctagataa ctgatcataa
5821 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
5881 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
5941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
6001 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
6061 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
6121 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
6181 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
6241 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
6301 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
6361 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
6421 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
6481 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
6541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
6601 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
6661 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
6721 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
6781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
6841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
6901 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
6961 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
7021 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
7081 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
7141 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
7201 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
7261 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
7321 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
7381 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
7441 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
7501 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
7561 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
7621 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
7681 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
7741 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
7801 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
7861 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
7921 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
7981 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
8041 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
8101 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
8161 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
8221 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
8281 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
8341 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
8401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
8461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
8521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
8581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
8641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
8701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
8761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
8821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
8881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
8941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
9001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
9061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
9121 ccgccatgca t
//
