| 质粒名称: | pCiES |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60010 |
| 存储实验室: | David Russell |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV beta |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7164 bp |
| 完整质粒大小: | 6801 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Env (foamy virus) |
| 物种来源: | human spumaretrovirus (foamy virus) |
| 插入大小: | 3390 bp |
| 基因突变: | -- |
| GenBank编号 | U21247.1 M54978.1 |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI (未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCiES was a gift from David Russell (Addgene plasmid # 60010 ; http://n2t.net/addgene:60010 ; RRID:Addgene_60010) |
| 参考文献部分: | Improved foamy virus vectors with minimal viral sequences. Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW. Mol Ther. 2002 Sep;6(3):321-8. S1525001602906726 [pii] PubMed 12231167 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCiES 6802 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Foamy virus helper plasmid expressing Env, driven by a CMV promoter.
Ampicillin resistant..
ACCESSION .
VERSION .
KEYWORDS pCiES
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6802)
AUTHORS Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW
TITLE Improved foamy virus vectors with minimal viral sequences.
JOURNAL Mol Ther. 2002 Sep;6(3):321-8.
PUBMED 12231167
REFERENCE 2 (bases 1 to 6802)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6802
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..330
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 485..505
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 531..555
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 672..768
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
polyA_signal 3941..4075
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3965..3984)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 4019..4038
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(4186..4202)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4186..4202)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4199..4221)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4210..4226
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4234..4264)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4279..4300
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4417..4434)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4588..5176)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4668..4687)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5347..6207)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5970..5989
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6208..6312)
/gene="bla"
/label=AmpR promoter
primer_bind 6380..6398
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6436..6458)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6558..6577
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6771..6793
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6785..6802
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6786..6802
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gccgcaatcg agtctagacc
841 cttaaggtgt tgaatccaag gactgttgtt attttggacc atcttggcaa caacagaact
901 gtaagtatag ataatttaaa acctacttct catcagaatg gcaccaccaa tgacactgca
961 acaatggatc atttggaaaa aaatgaataa agcgcatgag gcacttcaaa atacaacaac
1021 tgtgactgaa cagcagaagg aacaaattat actggacatt caaaatgaag aagtacaacc
1081 aactaggaga gataaattta gatatctgct ttatacttgt tgtgctacta gctcaagagt
1141 attggcctgg atgtttttag tttgtatatt gttaatcatt gttttggttt catgctttgt
1201 gactatatcc agaatacaat ggaataagga tattcaggta ttaggacctg taatagactg
1261 gaatgttact caaagagctg tttatcaacc cttacagact agaaggattg cacgttccct
1321 tagaatgcag catcctgttc caaaatatgt ggaggtaaat atgactagta ttccacaagg
1381 tgtatactat gaaccccatc cggaacccat agtggtgaag gagagggtcc tgggtctttc
1441 tcaaattctg atgattaatt cagaaaacat tgctaataat gctaatttga cacaagaagt
1501 aaagaagttg ttaactgaaa tggttaatga agaaatgcaa agtttgtcag atgtaatgat
1561 tgactttgaa attcctttag gagaccctcg tgatcaagaa caatatatac atagaaaatg
1621 ctatcaagaa tttgcaaatt gttatttagt aaaatataaa gaacccaaac cgtggcctaa
1681 ggagggcctt atagctgatc aatgcccatt accaggttac catgctggat taacctataa
1741 tagacagtct atttgggatt actatattaa agtggagagt attagacctg caaattggac
1801 aacaaagagt aaatatggac aagctagact aggaagtttt tatattccta gcagtctgag
1861 acaaatcaat gttagtcatg tactattctg tagtgatcaa ttatattcta aatggtataa
1921 tatagaaaat accatagaac aaaacgagcg gtttctgctt aataaactaa ataaccttac
1981 atctggaacc tcagtattga agaaaagagc tcttccgaag gattggagtt ctcaaggtaa
2041 aaatgctctg tttagagaaa tcaatgtgtt agatatctgc agtaaacctg aatctgtaat
2101 actattgaat acttcatact attccttctc tttatgggaa ggagattgta attttactaa
2161 agatatgatt tctcagttgg ttccagaatg tgatggattt tataacaatt ctaagtggat
2221 gcatatgcat ccatatgctt gtagattctg gagaagtaag aatgaaaaag aagaaactaa
2281 atgtagagat ggggaaacta agagatgtct gtattatcct ttatgggaca gtcccgaatc
2341 tacatatgat tttggttatt tagcatacca aaagaatttt ccttccccta tctgtataga
2401 acaacagaaa attagagatc aagattatga agtttattct ttgtatcaag aatgcaaaat
2461 agcttctaaa gcatatggaa ttgatacagt tttattctct ctaaagaatt ttcttaatta
2521 tacaggaact cctgtaaatg aaatgcctaa tgcaagagct tttgtaggcc taatagatcc
2581 caagtttcct ccttcctatc ccaatgttac tagggaacat tatacttcct gtaataatag
2641 gaaaagaaga agtgttgata ataactatgc taagttaagg tctatggggt atgcacttac
2701 aggagcagtg caaaccttat ctcaaatatc agatattaat gatgaaaact tacagcaagg
2761 aatatattta ttaagggatc atgtaataac cttaatggaa gctacattgc atgatatatc
2821 tgttatggaa ggaatgtttg ctgtacaaca tttgcataca catttgaatc atttgaagac
2881 aatgcttcta gaaagaagaa tagactggac ctatatgtct agtacttggc tacaacaaca
2941 attacagaaa tctgatgatg agatgaaagt aataaagaga attgctagaa gtttggtata
3001 ttatgttaaa caaacccata gttctcccac agctacagcc tgggagattg gattatatta
3061 tgaattggtt atacctaaac atatttactt gaataattgg aatgttgtca atataggtca
3121 cttagttaaa tcagctggac aattgactca tgtaactata gctcatcctt atgaaataat
3181 caataaggaa tgtgtagaga ctatatatct gcatcttgaa gactgcacaa gacaagatta
3241 tgtcatatgt gatgtggtaa agatagtgca gccttgtggc aatagctcag acacgagtga
3301 ttgtcctgtc tgggctgaag ctgtaaaaga accatttgtg caagtcaatc ctctgaaaaa
3361 cggaagttat ctggttttgg caagttccac agactgtcag atcccaccat atgttcctag
3421 catcgtgact gttaatgaaa caacgtcatg ctttggactg gactttaaaa ggccactggt
3481 tgcggaagaa agattgagct ttgagccacg actgccaaat ctacaactaa gattaccaca
3541 tttggttgga attattgcaa aaatcaaagg gataaaaata gaagtcacat cctctggaga
3601 aagtataaaa gagcagattg aaagagcaaa agctgagctc cttcgactgg acattcacga
3661 gggagatact cctgcctgga tacaacagct agctgcagca acaaaggacg tctggccagc
3721 agcagcttct gctctacaag gaattggtaa ctttttatct gggactgccc aaggaatatt
3781 tggaactgcc tttagtctct tgggatactt aaagcctatc ctaataggag taggggtcat
3841 tctcttggtt attcttatat ttaagattgt atcatggatt cctacgaaaa agaagaatca
3901 gtagcctcca cctctggaat tcggatcttg cggccgcggg gatccagaca tgataagata
3961 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
4021 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
4081 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttcgga
4141 tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc
4201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
4261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
4321 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4381 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4441 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4501 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4561 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4621 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4681 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4741 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4801 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4861 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4921 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4981 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
5041 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5101 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5161 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5221 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
5281 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5341 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5401 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5461 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5521 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5581 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5641 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
5701 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
5761 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5821 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5881 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5941 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
6001 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6061 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6121 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6181 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6241 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6301 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
6361 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
6421 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
6481 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
6541 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
6601 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
6661 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
6721 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
6781 acgttgtaaa acgacggcca gt
//
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