pPIC3.5K

价格:800元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pPIC3.5K, pPIC 3.5K
质粒类型: 毕赤酵母蛋白表达载体
表达水平: 高拷贝
启动子: AOX1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9004 bp
5' 测序引物及序列: 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´
3' 测序引物及序列: 3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´
载体标签: /
载体抗性: Ampicillin
筛选标记: HIS4
备注:
插入基因是必需包含起始密码子ATG。
产品目录号: K173-20
稳定性: 稳定 Stable
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pPIC3.5K载体图谱

pPIC3.5K多克隆位点

载体简介

pPIC3.5K是一个鉴定多拷贝基因插入到毕赤酵母基因组中的载体。

1. pPIC3.5K载体大小为9004 bp。

2. 在多克隆位点区域,拥有5个单一的限制性酶切位点:BamH I,Snab I,EcoR I,Avr II和Not I。

3.胞内表达。

4.载体构建时,需要包含一个ATG启动子,同时需要加入Kozak序列。 (Cavener and Stuart, 1991; Kozak, 1987; Kozak, 1990)

5. 毕赤酵母中使用HIS4基因筛选。

6.  将基因插入GS115和KM71的AOX1中,需要使用Sac I线性化质粒(GS115产生的基因型为His+ Mut+,KM71酵母产生的基因型为His+ MutS)。

7. 将基因插入GS115和KM71的HIS4中,需要使用Sal I线性化质粒(GS115产生的基因型为His+ Mut+,KM71酵母产生的基因型为His+ MutS)。

8. 将基因插入GS115的AOX1基因中,也可以使用Bgl II 限制酶线性化质粒,产生的酵母基因型为His+ MutS。

载体序列

LOCUS       pPIC3.5K    9004 bp     DNA  circular   SYN
DEFINITION  pPIC3.5K
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="pPIC3.5K"
                     /mol_type="other DNA"
     misc_feature    complement(1055..1075)
                     /label="3_AOX1_primer"
     CDS             complement(1708..4242)
                     /label="ORF frame 2"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
                     ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
                     TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
                     PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
                     KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
                     CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
                     AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
                     PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
                     NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT
                     ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
                     PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
                     AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV
                     DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
                     LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ
                     KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI*"
     gene            complement(4656..5471)
                     /label="KanR2"
                     /gene="KanR2"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
                     ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
                     TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
                     PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
                     KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
                     CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
                     AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
                     PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
                     NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT
                     ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
                     PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
                     AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV
                     DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
                     LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ
                     KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI*"
     CDS             complement(4656..5471)
                     /label="ORF frame 3"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_feature    complement(5636..5655)
                     /label="pBRrevBam_primer"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     CDS             complement(5637..6359)
                     /label="ORF frame 3"
                     /translation="MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV
                     GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS
                     FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT
                     IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT
                     PKQALMSPKWRARSSPSVMSAI*"
     misc_feature    complement(6641..6663)
                     /label="pGEX_3_primer"
                     /translation="MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV
                     GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS
                     FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT
                     IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT
                     PKQALMSPKWRARSSPSVMSAI*"
     rep_origin      complement(7060..7679)
                     /label="pBR322_origin"
                     /translation="MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV
                     GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS
                     FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT
                     IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT
                     PKQALMSPKWRARSSPSVMSAI*"
     gene            complement(7834..8694)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEV
                     GISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTS
                     FTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGIT
                     IPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPT
                     PKQALMSPKWRARSSPSVMSAI*"
     CDS             complement(7834..8694)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(8736..8764)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG
   61 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT
  121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC
  181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA
  241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA
  301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG
  361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT
  421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG
  481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT
  541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT
  601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT
  661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT
  721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT
  781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT
  841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA
  901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCTACGTAG AATTCCCTAG
  961 GGCGGCCGCG AATTAATTCG CCTTAGACAT GACTGTTCCT CAGTTCAAGT TGGGCACTTA
 1021 CGAGAAGACC GGTCTTGCTA GATTCTAATC AAGAGGATGT CAGAATGCCA TTTGCCTGAG
 1081 AGATGCAGGC TTCATTTTTG ATACTTTTTT ATTTGTAACC TATATAGTAT AGGATTTTTT
 1141 TTGTCATTTT GTTTCTTCTC GTACGAGCTT GCTCCTGATC AGCCTATCTC GCAGCTGATG
 1201 AATATCTTGT GGTAGGGGTT TGGGAAAATC ATTCGAGTTT GATGTTTTTC TTGGTATTTC
 1261 CCACTCCTCT TCAGAGTACA GAAGATTAAG TGAGAAGTTC GTTTGTGCAA GCTTATCGAT
 1321 AAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG
 1381 AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA
 1441 GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATA TCGTCCATTC CGACAGCATC
 1501 GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC
 1561 GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT
 1621 GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CTATCGAATC
 1681 TAAATGTAAG TTAAAATCTC TAAATAATTA AATAAGTCCC AGTTTCTCCA TACGAACCTT
 1741 AACAGCATTG CGGTGAGCAT CTAGACCTTC AACAGCAGCC AGATCCATCA CTGCTTGGCC
 1801 AATATGTTTC AGTCCCTCAG GAGTTACGTC TTGTGAAGTG ATGAACTTCT GGAAGGTTGC
 1861 AGTGTTAACT CCGCTGTATT GACGGGCATA TCCGTACGTT GGCAAAGTGT GGTTGGTACC
 1921 GGAGGAGTAA TCTCCACAAC TCTCTGGAGA GTAGGCACCA ACAAACACAG ATCCAGCGTG
 1981 TTGTACTTGA TCAACATAAG AAGAAGCATT CTCGATTTGC AGGATCAAGT GTTCAGGAGC
 2041 GTACTGATTG GACATTTCCA AAGCCTGCTC GTAGGTTGCA ACCGATAGGG TTGTAGAGTG
 2101 TGCAATACAC TTGCGTACAA TTTCAACCCT TGGCAACTGC ACAGCTTGGT TGTGAACAGC
 2161 ATCTTCAATT CTGGCAAGCT CCTTGTCTGT CATATCGACA GCCAACAGAA TCACCTGGGA
 2221 ATCAATACCA TGTTCAGCTT GAGACAGAAG GTCTGAGGCA ACGAAATCTG GATCAGCGTA
 2281 TTTATCAGCA ATAACTAGAA CTTCAGAAGG CCCAGCAGGC ATGTCAATAC TACACAGGGC
 2341 TGATGTGTCA TTTTGAACCA TCATCTTGGC AGCAGTAACG AACTGGTTTC CTGGACCAAA
 2401 TATTTTGTCA CACTTAGGAA CAGTTTCTGT TCCGTAAGCC ATAGCAGCTA CTGCCTGGGC
 2461 GCCTCCTGCT AGCACGATAC ACTTAGCACC AACCTTGTGG GCAACGTAGA TGACTTCTGG
 2521 GGTAAGGGTA CCATCCTTCT TAGGTGGAGA TGCAAAAACA ATTTCTTTGC AACCAGCAAC
 2581 TTTGGCAGGA ACACCCAGCA TCAGGGAAGT GGAAGGCAGA ATTGCGGTTC CACCAGGAAT
 2641 ATAGAGGCCA ACTTTCTCAA TAGGTCTTGC AAAACGAGAG CAGACTACAC CAGGGCAAGT
 2701 CTCAACTTGC AACGTCTCCG TTAGTTGAGC TTCATGGAAT TTCCTGACGT TATCTATAGA
 2761 GAGATCAATG GCTCTCTTAA CGTTATCTGG CAATTGCATA AGTTCCTCTG GGAAAGGAGC
 2821 TTCTAACACA GGTGTCTTCA AAGCGACTCC ATCAAACTTG GCAGTTAGTT CTAAAAGGGC
 2881 TTTGTCACCA TTTTGACGAA CATTGTCGAC AATTGGTTTG ACTAATTCCA TAATCTGTTC
 2941 CGTTTTCTGG ATAGGACGAC GAAGGGCATC TTCAATTTCT TGTGAGGAGG CCTTAGAAAC
 3001 GTCAATTTTG CACAATTCAA TACGACCTTC AGAAGGGACT TCTTTAGGTT TGGATTCTTC
 3061 TTTAGGTTGT TCCTTGGTGT ATCCTGGCTT GGCATCTCCT TTCCTTCTAG TGACCTTTAG
 3121 GGACTTCATA TCCAGGTTTC TCTCCACCTC GTCCAACGTC ACACCGTACT TGGCACATCT
 3181 AACTAATGCA AAATAAAATA AGTCAGCACA TTCCCAGGCT ATATCTTCCT TGGATTTAGC
 3241 TTCTGCAAGT TCATCAGCTT CCTCCCTAAT TTTAGCGTTC AACAAAACTT CGTCGTCAAA
 3301 TAACCGTTTG GTATAAGAAC CTTCTGGAGC ATTGCTCTTA CGATCCCACA AGGTGGCTTC
 3361 CATGGCTCTA AGACCCTTTG ATTGGCCAAA ACAGGAAGTG CGTTCCAAGT GACAGAAACC
 3421 AACACCTGTT TGTTCAACCA CAAATTTCAA GCAGTCTCCA TCACAATCCA ATTCGATACC
 3481 CAGCAACTTT TGAGTTGCTC CAGATGTAGC ACCTTTATAC CACAAACCGT GACGACGAGA
 3541 TTGGTAGACT CCAGTTTGTG TCCTTATAGC CTCCGGAATA GACTTTTTGG ACGAGTACAC
 3601 CAGGCCCAAC GAGTAATTAG AAGAGTCAGC CACCAAAGTA GTGAATAGAC CATCGGGGCG
 3661 GTCAGTAGTC AAAGACGCCA ACAAAATTTC ACTGACAGGG AACTTTTTGA CATCTTCAGA
 3721 AAGTTCGTAT TCAGTAGTCA ATTGCCGAGC ATCAATAATG GGGATTATAC CAGAAGCAAC
 3781 AGTGGAAGTC ACATCTACCA ACTTTGCGGT CTCAGAAAAA GCATAAACAG TTCTACTACC
 3841 GCCATTAGTG AAACTTTTCA AATCGCCCAG TGGAGAAGAA AAAGGCACAG CGATACTAGC
 3901 ATTAGCGGGC AAGGATGCAA CTTTATCAAC CAGGGTCCTA TAGATAACCC TAGCGCCTGG
 3961 GATCATCCTT TGGACAACTC TTTCTGCCAA ATCTAGGTCC AAAATCACTT CATTGATACC
 4021 ATTATTGTAC AACTTGAGCA AGTTGTCGAT CAGCTCCTCA AATTGGTCCT CTGTAACGGA
 4081 TGACTCAACT TGCACATTAA CTTGAAGCTC AGTCGATTGA GTGAACTTGA TCAGGTTGTG
 4141 CAGCTGGTCA GCAGCATAGG GAAACACGGC TTTTCCTACC AAACTCAAGG AATTATCAAA
 4201 CTCTGCAACA CTTGCGTATG CAGGTAGCAA GGGAAATGTC ATACTTGAAG TCGGACAGTG
 4261 AGTGTAGTCT TGAGAAATTC TGAAGCCGTA TTTTTATTAT CAGTGAGTCA GTCATCAGGA
 4321 GATCCTCTAC GCCGGACGCA TCGTGGCCGA CCTGCAGGGG GGGGGGGGGC GCTGAGGTCT
 4381 GCCTCGTGAA GAAGGTGTTG CTGACTCATA CCAGGCCTGA ATCGCCCCAT CATCCAGCCA
 4441 GAAAGTGAGG GAGCCACGGT TGATGAGAGC TTTGTTGTAG GTGGACCAGT TGGTGATTTT
 4501 GAACTTTTGC TTTGCCACGG AACGGTCTGC GTTGTCGGGA AGATGCGTGA TCTGATCCTT
 4561 CAACTCAGCA AAAGTTCGAT TTATTCAACA AAGCCGCCGT CCCGTCAAGT CAGCGTAATG
 4621 CTCTGCCAGT GTTACAACCA ATTAACCAAT TCTGATTAGA AAAACTCATC GAGCATCAAA
 4681 TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC
 4741 TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG
 4801 TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA
 4861 AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAAAGC
 4921 TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA
 4981 CTCGCATCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA
 5041 TCGCTGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC
 5101 AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT
 5161 TTCCCGGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG
 5221 ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGTC TGACCATCTC ATCTGTAACA
 5281 TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA
 5341 TACAATCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA
 5401 TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCCTCG AGCAAGACGT TTCCCGTTGA
 5461 ATATGGCTCA TAACACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT TATTGTTCAT
 5521 GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC AACGTGGCTT
 5581 TCCCCCCCCC CCCTGCAGGT CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT
 5641 ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT
 5701 TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG
 5761 CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC
 5821 CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGAGTATCTA TGATTGGAAG TATGGGAATG
 5881 GTGATACCCG CATTCTTCAG TGTCTTGAGG TCTCCTATCA GATTATGCCC AACTAAAGCA
 5941 ACCGGAGGAG GAGATTTCAT GGTAAATTTC TCTGACTTTT GGTCATCAGT AGACTCGAAC
 6001 TGTGAGACTA TCTCGGTTAT GACAGCAGAA ATGTCCTTCT TGGAGACAGT AAATGAAGTC
 6061 CCACCAATAA AGAAATCCTT GTTATCAGGA ACAAACTTCT TGTTTCGAAC TTTTTCGGTG
 6121 CCTTGAACTA TAAAATGTAG AGTGGATATG TCGGGTAGGA ATGGAGCGGG CAAATGCTTA
 6181 CCTTCTGGAC CTTCAAGAGG TATGTAGGGT TTGTAGATAC TGATGCCAAC TTCAGTGACA
 6241 ACGTTGCTAT TTCGTTCAAA CCATTCCGAA TCCAGAGAAA TCAAAGTTGT TTGTCTACTA
 6301 TTGATCCAAG CCAGTGCGGT CTTGAAACTG ACAATAGTGT GCTCGTGTTT TGAGGTCATC
 6361 TTTGTATGAA TAAATCTAGT CTTTGATCTA AATAATCTTG ACGAGCCAAG GCGATAAATA
 6421 CCCAAATCTA AAACTCTTTT AAAACGTTAA AAGGACAAGT ATGTCTGCCT GTATTAAACC
 6481 CCAAATCAGC TCGTAGTCTG ATCCTCATCA ACTTGAGGGG CACTATCTTG TTTTAGAGAA
 6541 ATTTGCGGAG ATGCGATATC GAGAAAAAGG TACGCTGATT TTAAACGTGA AATTTATCTC
 6601 AAGATCTCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
 6661 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
 6721 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG
 6781 GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT
 6841 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC
 6901 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
 6961 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
 7021 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
 7081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
 7141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
 7201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
 7261 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
 7321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
 7381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
 7441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
 7501 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
 7561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
 7621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
 7681 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
 7741 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
 7801 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
 7861 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
 7921 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC
 7981 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
 8041 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
 8101 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGGCATCGT
 8161 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
 8221 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
 8281 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
 8341 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
 8401 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA
 8461 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
 8521 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
 8581 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
 8641 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
 8701 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
 8761 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
 8821 ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC
 8881 GAGGCCCTTT CGTCTTCAAG AATTAATTCT CATGTTTGAC AGCTTATCAT CGATAAGCTG
 8941 ACTCATGTTG GTATTGTGAA ATAGACGCAG ATCGGGAACA CTGAAAAATA ACAGTTATTA
 9001 TTCG
//

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