pEarleyGate203

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pEarleyGate203; pEarleyGate 203
质粒类型: Gateway载体;植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: Gateway
启动子: 35S
载体大小: 11783 bp
5' 测序引物及序列: 35S promoter:CTATCCTTCGCAAGACCCTTC
3' 测序引物及序列: --
载体标签: Myc(N ter)
载体抗性: 卡那霉素、氯霉素(仅空载体)
筛选标记: 草丁膦(磷丝菌素)phosphinothricin、
克隆菌株: DB3.1菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEarleyGate203载体图谱



载体简介

载体序列

LOCUS       Exported               11783 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  Gateway(R)-compatible plant transformation vector with an N-terminal
            Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    pEarleyGate 203
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 7891.
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /note="MAS terminator"
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase草丁膦(磷丝菌素) 
                     乙酰转移酶"
                     /note="BlpR"
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /note="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2681..2735
                     /note="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2737..2739
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             2740..2769
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     protein_bind    2773..2892
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2922..2952
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3006..3665
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             4007..4312
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4353..4477)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4554..5261
                     /note="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    5505..5529
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6829..7458
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7887..8960
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9026..9220
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9564..9704
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9890..10478)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10565..11359)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgg aacagaaact gatctctgaa
     2761 gaagatctga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
     2821 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     2881 catatccagt catattggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
     2941 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
     3001 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     3061 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     3121 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     3181 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     3241 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     3301 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     3361 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     3421 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     3481 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     3541 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     3601 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     3661 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     3721 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
     3781 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     3841 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     3901 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     3961 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
     4021 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     4081 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     4141 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     4201 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     4261 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     4321 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     4381 agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     4441 tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc taggtgagtc tagagagtta
     4501 attctaggtg agtctagaga gttaattaag acccgggact agtccctaga gtcctgcttt
     4561 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
     4621 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
     4681 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
     4741 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
     4801 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
     4861 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
     4921 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
     4981 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
     5041 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
     5101 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
     5161 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     5221 gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag gcatgcaagc
     5281 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5341 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5401 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg
     5461 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
     5521 cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa
     5581 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     5641 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     5701 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     5761 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     5821 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     5881 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     5941 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     6001 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     6061 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     6121 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     6181 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     6241 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     6301 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     6361 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     6421 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     6481 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     6541 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     6601 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     6661 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     6721 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     6781 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     6841 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     6901 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     6961 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     7021 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     7081 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     7141 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     7201 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     7261 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     7321 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     7381 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     7441 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     7501 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     7561 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     7621 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     7681 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     7741 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     7801 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     7861 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     7921 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     7981 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     8041 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     8101 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     8161 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     8221 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     8281 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     8341 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     8401 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     8461 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     8521 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     8581 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     8641 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     8701 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     8761 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     8821 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     8881 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     8941 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     9001 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     9061 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     9121 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     9181 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     9241 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     9301 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     9361 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     9421 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     9481 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     9541 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     9601 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     9721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    10561 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    10621 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    10681 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    10741 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    10801 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    10861 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    10921 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    10981 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    11041 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    11101 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    11161 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    11221 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    11281 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    11341 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    11401 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    11461 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    11521 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    11581 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    11641 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    11701 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    11761 tcggggagct gttggctggc tgg
//

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