| 质粒名称: | pGC1::YC3.60 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 107061 |
| 存储实验室: | Julian Schroeder |
|
载体骨架基本信息 |
|
| 载体名称: | pGreenII 0179 |
| 空载体大小: | 9069bp |
| 完整质粒大小: | 9085 bp |
| 载体类型: | 植物细胞表达 |
| 筛选标记: | Hygromycin |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | YC3.6 / yellow cameleon 3.6 |
| 物种来源: | Synthetic |
| 插入大小: | 1962bp |
| 启动子: | GC1 (At1g22690) |
| 克隆方法: | 未知 |
| 5’测序引物 | gtaaaacgacggccagt |
| 3’测序引物 | gtcatagctgtttcctg |
|
文献引用方法 |
|
| 材料和方法部分: |
pGC1::YC3.60 was a gift from Julian Schroeder (Addgene plasmid # 107061 ; http://n2t.net/addgene:107061 ; RRID:Addgene_107061)
|
| 参考文献部分: | Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool. Yang Y, Costa A, Leonhardt N, Siegel RS, Schroeder JI. Plant Methods. 2008 Feb 19;4:6. doi: 10.1186/1746-4811-4-6. 10.1186/1746-4811-4-6 PubMed 18284694 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGC1::YC3.60 9085 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expressing Ca sensor yellow cameloen 3.6 under guard cell specific
promoter.
ACCESSION .
VERSION .
KEYWORDS pGC1::YC3.60
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9085)
AUTHORS Yang Y, Costa A, Leonhardt N, Siegel RS, Schroeder JI
TITLE Isolation of a strong Arabidopsis guard cell promoter and its
potential as a research tool.
JOURNAL Plant Methods. 2008 Feb 19;4:6. doi: 10.1186/1746-4811-4-6.
PUBMED 18284694
REFERENCE 2 (bases 1 to 9085)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9085
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2859..2936
/codon_start=1
/product="calmodulin-binding peptide"
/label=CBP
/note="derived from skeletal muscle myosin light chain
kinase; binds calmodulin with nanomolar affinity in the
presence of calcium"
/translation="KRRWKKNFIAVSAANRFKKISSSGAL"
CDS 2943..3677
/codon_start=1
/product="circularly permuted form of the Venus yellow
fluorescent protein (Nagai et al., 2004)"
/label=cp173Venus
/note="efficient FRET acceptor for CFP"
/translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYITADKQKNGIKANFKIRHNIE"
terminator 3701..3953
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3962..3978)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4026..4046)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4026..4044)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4065..4081)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4065..4081)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4078..4100)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4089..4105
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4113..4143)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4158..4179
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4296..4313)
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 4418..4442
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(4533..5121)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4613..4632)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5292..6107)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 6015..6034
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 6099..6118
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 6398..6833
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 6960..6982
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
polyA_signal 6990..7166
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(7398..8423)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(8443..8788)
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind complement(8480..8500)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
primer_bind 8991..9013
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9006..9022
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9032..9051
/label=T7
/note="T7 promoter, forward primer"
promoter 9032..9050
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gagtaaagat tcagtaaccc gatgctcctg ctcttcctca agaccttcct tgattcgccg
61 ccggtatgtt ctccgtctgt ggtagcgcct ttggaacact ctaccaacgc cgccatgaaa
121 ggatctctca tggccgcagg ggacgtgttc ttcttacatc tggtgttagg gctatggtta
181 ctccagtgag gagggagagg caagaggttg cttaatgatt cgtttttccg gtgatacgag
241 aactctttag gtttaccggg aagcttttcc catgaaaatg ggatgccaag tggatggaga
301 ggagttgccg gagagttgcc ggagaatagg agggaattgg aggaggagga agagagtgat
361 cgccgggttg aaatgttaac cgtcgaggag aatttgaccg agttggatcg tctagtaggt
421 acaattcggg tccttggcga agtatccatt caaaatagtg tttagttttg gacttgagaa
481 cttgttgtct ctttgatctc ttttatataa aactttggac gtgtaggaca aacttgtcaa
541 cataagaaac aaaatggttg caacagagag gatgaattta taagttttca acaccgcttt
601 tcttattaga cggacaacaa tctatagtgg agtaaatttt tatttttggt aaaatggtta
661 gtgaattcaa atatctaaat tttgtgactc actaacatta acaaatatgc ataagacata
721 aaaaaaagaa agaataattc ttatgaaaca agaaaaaaaa cctatacaat caatctttag
781 gaattgacga tgtagaattg tagatgataa attttctcaa atatagatgg gcctaatgaa
841 gggtgccgct tattggatct gacccatttt gaggacatta atattttcat tggttataag
901 ccttttaatc aaaattgtca ttaaattgat gtctccctct cgggtcattt tcctttctcc
961 ctcacaatta atgtagactt tagcaatttg cacgctgtgc tttgtcttta tatttagtaa
1021 cacaaacatt ttgacttgtc ttgtagagtt tttctctttt atttttctat ccaatatgaa
1081 aactaaaagt gttctcgtat acatatatta aaattaaaga aacctatgaa aacaccaata
1141 caaatgcgat attgttttca gttcgacgtt tcatgtttgt tagaaaattt ctaatgacgt
1201 ttgtataaaa tagacaatta aacgccaaac actacatctg tgttttcgaa caatattgcg
1261 tctgcgtttc cttcatctat ctctctcagt gtcacaatgt ctgaactaag agacagctgt
1321 aaactatcat taagacataa actaccaaag tatcaagcta atgtaaaaat tactctcatt
1381 tccacgtaac aaattgagtt agcttaagat attagtgaaa ctaggtttga attttcttct
1441 tcttcttcca tgcatcctcc gaaaaaaggg aaccaatcaa aactgtttgc atatcaaact
1501 ccaacacttt acagcaaatg caatctataa tctgtgattt atccaataaa aacctgtgat
1561 ttatgtttgg ctccagcgat gaaagtctat gcatgtgatc tctatccaac atgagtaatt
1621 gttcagaaaa taaaaagtag ctgaaatgta tctatataaa gaatcatcca caagtactat
1681 tttcacacac tacttcaaaa tcactactca agaaatccat ggtgagcaag ggcgaggagc
1741 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaggt
1801 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
1861 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
1921 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
1981 ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac ggcaactaca
2041 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
2101 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
2161 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc cacttcaaga
2221 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
2281 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
2341 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
2401 cccgcatgca tgaccaactg acagaagagc agattgcaga gttcaaagaa gccttctcat
2461 tattcgacaa ggatggggac ggcaccatca ccacaaagga acttggcacc gttatgaggt
2521 cgcttggaca aaacccaacg gaagcagaat tgcaggatat gatcaatgaa gtcgatgctg
2581 atggcaatgg aacgatttac tttcctgaat ttcttactat gatggctaga aaaatgaagg
2641 acacagacag cgaagaggaa atccgagaag cattccgtgt ttttgacaag gatgggaacg
2701 gctacatcag cgctgctcag ttacgtcacg tcatgacaaa cctcggggag aagttaacag
2761 atgaagaagt tgatgaaatg ataagggaag cagatatcga tggtgatggc caagtaaact
2821 atgaagagtt tgtacaaatg atgacagcaa agggggggaa gaggcgctgg aagaaaaact
2881 tcattgccgt cagcgctgcc aaccggttca agaagatctc cagctccggg gcactggagc
2941 tcatggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
3001 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
3061 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
3121 tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag ggcgaggagc
3181 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
3241 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
3301 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
3361 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
3421 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
3481 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
3541 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
3601 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
3661 tccgccacaa catcgagtaa gaattcgatc gaatttcccc gatcgttcaa acatttggca
3721 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
3781 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
3841 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
3901 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
3961 cactggccgt cgttttacaa cgtcgtgacg cggccgccac cgcggtggag ctccagcttt
4021 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
4081 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
4141 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
4201 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
4261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
4321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
4381 gaatcagggg ataacgcagg aaagaacatg aaggccttga caggatatat tggcgggtaa
4441 actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc
4501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
4561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
4621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
4681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
4741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
4801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
4861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
4921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
4981 tttggtatct gcgctctgct gaagccagtt accttcggaa gaagagttgg tagctcttga
5041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
5101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
5161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
5221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgtgtaacat
5281 tggtctagtg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
5341 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
5401 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
5461 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
5521 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
5581 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
5641 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
5701 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
5761 gaatcaggat attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt
5821 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
5881 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
5941 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct
6001 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
6061 tttaatcgcg gccttgagca agacgtttcc cgttgaatat ggctcataac accccttgta
6121 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
6181 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
6241 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
6301 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
6361 actttctggc tggatgatgg ggcgattcag gcgatcccca tccaacagcc cgccgtcgag
6421 cgggcttttt tatccccgga agcctgtgga tagagggtag ttatccacgt gaaaccgcta
6481 atgccccgca aagccttgat tcacggggct ttccggcccg ctccaaaaac tatccacgtg
6541 aaatcgctaa tcagggtacg tgaaatcgct aatcggagta cgtgaaatcg ctaataaggt
6601 cacgtgaaat cgctaatcaa aaaggcacgt gagaacgcta atagcccttt cagatcaaca
6661 gcttgcaaac acccctcgct ccggcaagta gttacagcaa gtagtatgtt caattagctt
6721 ttcaattatg aatatatata tcaattattg gtcgcccttg gcttgtggac aatgcgctac
6781 gcgcaccggc tccgcccgtg gacaaccgca agcggttgcc caccgtcgag cgcctttgcc
6841 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
6901 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagatctt
6961 ggcaggatat attgtggtgt aacgttatcg atctggattt tagtactgga ttttggtttt
7021 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
7081 cttatatgct caacacatga gcgaaaccct ataagaaccc taatttccct tatcgggaaa
7141 ctactcacac attatttatg gagaaaatag agagagatag atttgtagag agagactggt
7201 gatttcagcg taccgaatta attcctgaat cactgcgacc ggccctcccg cgacccagcc
7261 gagcgagctt agcgaactgt ggacgagaac tgtgccacca agcgtaaggc cgttctctcg
7321 catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg
7381 ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca
7441 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
7501 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
7561 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
7621 cggagcatat acgcccggag ccttggcgat cctgcaagct ccggatgcct ccgctcgaag
7681 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
7741 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
7801 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
7861 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
7921 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
7981 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
8041 ctaagatcgg ccgcagcgat agcatccata gcctccgcga ccggctgaag aacagcgggc
8101 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
8161 gtcaggctct cgctgaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
8221 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
8281 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
8341 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
8401 gcggtgagtt caggcttttt catagggggg atcagcttgg gctgtcctct ccaaatgaaa
8461 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
8521 tacgtcagtg gagatgtcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
8581 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
8641 atcttgaatg atagcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
8701 ttctactgtc ctttcgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
8761 cgaaattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
8821 gacatttttg gagtaggggt acgataacat taacgcttac aatttccatt cgccattcag
8881 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
8941 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
9001 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
9061 taccgggccc cccctcgagg tcgac
//
