| 质粒名称: | pDmelOR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 126476 |
| 存储实验室: | Bill Hansson |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV-BI |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4111 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | DmelOrco,Orco,Odorant receptor co-receptor |
| 物种来源: | D. melanogaster (fly) |
| 插入大小: | 1509 bp |
| 基因突变: | codon optimization for H. sapiens; includes a recombinant β-globin/IgG chimeric intron to inhibit its functional expression in bacteria |
| 启动子 | CMV |
| 标签/融合蛋白: | Myc（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | HindIII(未破坏) |
| 5' 测序引物 | GTGTACGGTGGGAGGTCTATATAAG |
| 3' 测序引物 | GTGGTATGGCTGATTATGATCCTCT |
|
文献引用方法 |
|
| 材料和方法部分: | pDmelOR was a gift from Bill Hansson (Addgene plasmid # 126476 ; http://n2t.net/addgene:126476 ; RRID:Addgene_126476) |
| 参考文献部分: | Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells. Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, Lavista-Llanos S. Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062. 10.1093/chemse/bjz062 PubMed 31504297 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDmelOR 5709 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Vector for the functional expression of insect ORs in mammalian
cells, with a human codon-optimized version of D.melanogaster Orco
(hOrco) with an N-terminal myc tag and a beta-globin/IgG chimeric
intron.
ACCESSION .
VERSION .
KEYWORDS pDmelOR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5709)
AUTHORS Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS,
Lavista-Llanos S
TITLE Optimization of Insect Odorant Receptor Trafficking and Functional
Expression Via Transient Transfection in HEK293 Cells.
JOURNAL Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
PUBMED 31504297
REFERENCE 2 (bases 1 to 5709)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5709
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 110..191
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 494..797
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 798..1001
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 952..972
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 1035..1044
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1044..1073
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
intron 2289..2421
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 2404..2423
/label=pMT2-F
/note="Synthetic intron, forward primer"
polyA_signal 2802..2883
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2894..2911)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin 3019..3474
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3106..3125)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3316..3337
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3541..3560)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3660..3682
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3720..3738)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3806..3910
/gene="bla"
/label=AmpR promoter
CDS 3911..4771
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4129..4148)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4942..5530
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5431..5450
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
61 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcgg cagtgaaaaa
121 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
181 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
241 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
301 tagactgcag cctcaggaga tctgggcccc cgcggcatat gaccggtgaa ttctccaggc
361 gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta cacgccacct
421 cgacatactc gagtagttat taatagtaat caattacggg gtcattagtt catagcccat
481 atatggagtt ccgcgttaca taacttacgg taaatggctc gcctggctga ccgcccaacg
541 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
601 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
661 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
721 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
781 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
841 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
901 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
961 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
1021 tccgctaggg atccgccacc atggaacaga aactgatctc tgaagaagac ctggctagca
1081 caactagcat gcaacccagt aaatatacag gcctggtggc cgatctcatg cccaacataa
1141 gggcaatgaa atacagcggg ctctttatgc acaactttac gggagggtcc gccttcatga
1201 agaaagtcta cagcagcgta catctcgttt tccttctgat gcagttcacc ttcattctgg
1261 tcaatatggc ccttaatgcc gaagaggtga atgagctttc tgggaatacc atcaccacac
1321 tgttcttcac acactgcatc acaaagttca tctacctggc agtgaaccag aagaactttt
1381 accgtacact gaacatctgg aatcaagtga acacccaccc tctgtttgcc gaatcagatg
1441 ccagatacca cagtattgca ctcgccaaga tgaggaaact cttctttctt gtgatgctga
1501 caacagtggc tagtgctact gcctggacca ctatcacgtt ctttggcgat tctgtgaaaa
1561 tggtggtaga ccatgaaacc aacagcagca tccctgtcga gattccaaga cttccgatta
1621 agtcctttta tccctggaat gcaagtcatg gcatgttcta catgatctcc ttcgccttcc
1681 aaatctacta cgtgctgttt agcatgatac acagcaacct ctgtgacgtg atgttctgct
1741 cttggctgat atttgcctgt gaacagctcc agcatctgaa aggcattatg aagccactga
1801 tggaactgtc agcctcactt gacacttatc gcccaaattc tgctgctctc tttaggtcac
1861 tctcagctaa tagcaaatcc gagttgattc acaacgagga gaaagaccct gggactgaca
1921 tggacatgtc tggcatttat agcagcaaag ccgactgggg agcacagttt cgggcacctt
1981 ctacactcca gtcctttggc ggtaatggag gaggaggtaa tgggctggtt aacggtgcta
2041 accccaacgg tctgaccaag aaacaggaga tgatggtgcg atctgcgatc aagtactggg
2101 tcgaacgcca taagcacgtg gttagactgg ttgctgctat aggcgatacc tacggtgccg
2161 ctttgctgtt gcatatgctg actagcacga tcaagttgac tctgttggca tatcaagcca
2221 ccaagatcaa tggagtgaac gtatatgcgt ttacggttgt ggggtatctg ggctatgctc
2281 tggcccaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
2341 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
2401 actttgcctt tctctccaca ggtgtttcac ttttgcattt tcggcaatcg gctgatagag
2461 gagagttcct ctgttatgga ggcagcgtac agttgccact ggtacgatgg aagcgaagaa
2521 gccaagactt tcgttcagat tgtgtgtcag cagtgtcaga aggctatgtc catctcaggg
2581 gcgaaattct tcaccgtgtc cttggatctg tttgcctccg tactgggcgc agtcgtcacc
2641 tatttcatgg tcctggtcca actgaagtga aagcttgtcg acgatatctc cagaggatca
2701 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
2761 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
2821 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
2881 tgccccgagc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
2941 tatcagctca ctcaaaggcg gtaatacggt tatccagatc gcccttccca acagttgcgc
3001 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
3061 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
3121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
3181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
3241 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
3301 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
3361 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
3421 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
3481 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
3541 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
3601 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
3661 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
3721 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
3781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
3841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
3901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
3961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
4021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
4081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
4141 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
4201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
4261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
4321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
4381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
4441 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
4501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
4561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
4621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
4681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
4741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
4801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
4861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
4921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
4981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
5041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
5101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
5161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
5221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
5281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
5341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
5401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
5461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
5521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
5581 tgctcacatg gctcgacaga tcttcaatat tggccattag ccatattatt cattggttat
5641 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
5701 tacatttat
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pGDP1 NDM-1
- 下一篇：pGDP2 MCR-1
