| 质粒名称: | pAAV2/9n |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112865 |
| 存储实验室: | James M. Wilson |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rep2/Cap9 |
| 物种来源: | AAV |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Truncated P5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | KS primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV2/9n was a gift from James M. Wilson (Addgene plasmid # 112865 ; http://n2t.net/addgene:112865 ; RRID:Addgene_112865) |
买家导航
pAAV29n载体基本信息
pAAV29n载体图谱质粒图谱和多克隆位点信息
pAAV29n载体简介
pAAV29n载体序列
LOCUS pAAV2/9n 7330 bp ds-DNA circular SYN 01-OCT-2019
DEFINITION AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION .
VERSION .
KEYWORDS pAAV2/9n
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7330)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7330)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 1, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7330
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 17..39
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 31..48
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 32..48
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 58..77
/label=T7
/note="T7 promoter, forward primer"
promoter 58..76
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 105..121
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 106..122
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4574..4594)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4574..4592)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4613..4629)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4613..4629)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4626..4648)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4637..4653
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4661..4691)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4706..4727
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4844..4861)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5015..5603)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5095..5114)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5774..6634)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6397..6416
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6635..6739)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6765..7220)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6902..6923)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7114..7133
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
61 tacgactcac tatagggcga attgggtacc gggccccccc tcgatcgagg tcgacggtat
121 cgggggagct cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc
181 cggggtttta cgagattgtg attaaggtcc ccagcgacct tgacgagcat ctgcccggca
241 tttctgacag ctttgtgaac tgggtggccg agaaggaatg ggagttgccg ccagattctg
301 acatggatct gaatctgatt gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg
361 actttctgac ggaatggcgc cgtgtgagta aggccccgga ggctcttttc tttgtgcaat
421 ttgagaaggg agagagctac ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat
481 ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag agaatttacc
541 gcgggatcga gccgactttg ccaaactggt tcgcggtcac aaagaccaga aatggcgccg
601 gaggcgggaa caaggtggtg gatgagtgct acatccccaa ttacttgctc cccaaaaccc
661 agcctgagct ccagtgggcg tggactaata tggaacagta tttaagcgcc tgtttgaatc
721 tcacggagcg taaacggttg gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc
781 agaacaaaga gaatcagaat cccaattctg atgcgccggt gatcagatca aaaacttcag
841 ccaggtacat ggagctggtc gggtggctcg tggacaaggg gattacctcg gagaagcagt
901 ggatccagga ggaccaggcc tcatacatct ccttcaatgc ggcctccaac tcgcggtccc
961 aaatcaaggc tgccttggac aatgcgggaa agattatgag cctgactaaa accgcccccg
1021 actacctggt gggccagcag cccgtggagg acatttccag caatcggatt tataaaattt
1081 tggaactaaa cgggtacgat ccccaatatg cggcttccgt ctttctggga tgggccacga
1141 aaaagttcgg caagaggaac accatctggc tgtttgggcc tgcaactacc gggaagacca
1201 acatcgcgga ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg
1261 agaactttcc cttcaacgac tgtgtcgaca agatggtgat ctggtgggag gaggggaaga
1321 tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg
1381 accagaaatg caagtcctcg gcccagatag acccgactcc cgtgatcgtc acctccaaca
1441 ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag cagccgttgc
1501 aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt gggaaggtca
1561 ccaagcagga agtcaaagac tttttccggt gggcaaagga tcacgtggtt gaggtggagc
1621 atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt gacgcagata
1681 taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag
1741 cttcgatcaa ctacgcagac aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc
1801 tgatgctgtt tccctgcaga caatgcgaga gaatgaatca gaattcaaat atctgcttca
1861 ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg
1921 tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca tatcatggga aaggtgccag
1981 acgcttgcac tgcctgcgat ctggtcaatg tggatttgga tgactgcatc tttgaacaat
2041 aaatgattta aatcaggtat ggctgccgat ggttatcttc cagattggct cgaggacaac
2101 cttagtgaag gaattcgcga gtggtgggct ttgaaacctg gagcccctca acccaaggca
2161 aatcaacaac atcaagacaa cgctcgaggt cttgtgcttc cgggttacaa ataccttgga
2221 cccggcaacg gactcgacaa gggggagccg gtcaacgcag cagacgcggc ggccctcgag
2281 cacgacaagg cctacgacca gcagctcaag gccggagaca acccgtacct caagtacaac
2341 cacgccgacg ccgagttcca ggagcggctc aaagaagata cgtcttttgg gggcaacctc
2401 gggcgagcag tcttccaggc caaaaagagg cttcttgaac ctcttggtct ggttgaggaa
2461 gcggctaaga cggctcctgg aaagaagagg cctgtagagc agtctcctca ggaaccggac
2521 tcctccgcgg gtattggcaa atcgggtgca cagcccgcta aaaagagact caatttcggt
2581 cagactggcg acacagagtc agtcccagac cctcaaccaa tcggagaacc tcccgcagcc
2641 ccctcaggtg tgggatctct tacaatggct tcaggtggtg gcgcaccagt ggcagacaat
2701 aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt ggcattgcga ttcccaatgg
2761 ctgggggaca gagtcatcac caccagcacc cgaacctggg ccctgcccac ctacaacaat
2821 cacctctaca agcaaatctc caacagcaca tctggaggat cttcaaatga caacgcctac
2881 ttcggctaca gcaccccctg ggggtatttt gacttcaaca gattccactg ccacttctca
2941 ccacgtgact ggcagcgact catcaacaac aactggggat tccggcctaa gcgactcaac
3001 ttcaagctct tcaacattca ggtcaaagag gttacggaca acaatggagt caagaccatc
3061 gccaataacc ttaccagcac ggtccaggtc ttcacggact cagactatca gctcccgtac
3121 gtgctcgggt cggctcacga gggctgcctc ccgccgttcc cagcggacgt tttcatgatt
3181 cctcagtacg ggtatctgac gcttaatgat ggaagccagg ccgtgggtcg ttcgtccttt
3241 tactgcctgg aatatttccc gtcgcaaatg ctaagaacgg gtaacaactt ccagttcagc
3301 tacgagtttg agaacgtacc tttccatagc agctacgctc acagccaaag cctggaccga
3361 ctaatgaatc cactcatcga ccaatacttg tactatctct caaagactat taacggttct
3421 ggacagaatc aacaaacgct aaaattcagt gtggccggac ccagcaacat ggctgtccag
3481 ggaagaaact acatacctgg acccagctac cgacaacaac gtgtctcaac cactgtgact
3541 caaaacaaca acagcgaatt tgcttggcct ggagcttctt cttgggctct caatggacgt
3601 aatagcttga tgaatcctgg acctgctatg gccagccaca aagaaggaga ggaccgtttc
3661 tttcctttgt ctggatcttt aatttttggc aaacaaggaa ctggaagaga caacgtggat
3721 gcggacaaag tcatgataac caacgaagaa gaaattaaaa ctactaaccc ggtagcaacg
3781 gagtcctatg gacaagtggc cacaaaccac cagagtgccc aagcacaggc gcagaccggc
3841 tgggttcaaa accaaggaat acttccgggt atggtttggc aggacagaga tgtgtacctg
3901 caaggaccca tttgggccaa aattcctcac acggacggca actttcaccc ttctccgctg
3961 atgggagggt ttggaatgaa gcacccgcct cctcagatcc tcatcaaaaa cacacctgta
4021 cctgcggatc ctccaacggc cttcaacaag gacaagctga actctttcat cacccagtat
4081 tctactggcc aagtcagcgt ggagatcgag tgggagctgc agaaggaaaa cagcaagcgc
4141 tggaacccgg agatccagta cacttccaac tattacaagt ctaataatgt tgaatttgct
4201 gttaatactg aaggtgtata tagtgaaccc cgccccattg gcaccagata cctgactcgt
4261 aatctgtaat tgcttgttaa tcaataaacc gtttaattcg tttcagttga actttggtct
4321 ctgcgaaggg cgaattcgtt taaacctgca ggactagagg tcctgtatta gaggtcacgt
4381 gagtgttttg cgacattttg cgacaccatg tggtcacgct gggtatttaa gcccgagtga
4441 gcacgcaggg tctccatttt gaagcgggag gtttgaacgc gcagccgcca agccgaattc
4501 tgcagatatc catcacactg gcggccgctc gactagagcg gccgccaccg cggtggagct
4561 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
4621 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
4681 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
4741 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
4801 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
4861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
4921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
4981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
5041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
5461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
5701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
5761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
5821 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
5881 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
5941 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
6001 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
6061 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
6121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
6181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
6241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
6301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
6361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
6421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
6481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
6541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
6601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
6661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
6721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
6781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
6841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
6901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
6961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
7021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
7081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
7141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
7201 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
7261 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
7321 attaagttgg
//
