pGL4.71[hRlucP]

价格:1500元

联系方式:I47-825O-882O

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pGL4.71载体基本信息

出品公司: Promega
载体名称: pGL4.71 ; pGL4.71[hRlucP]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 3653 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRlucP
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.71[hRlucP]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRlucP。
产品目录号: E6891
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.71载体质粒图谱和多克隆位点信息

pGL4.71载体图谱



pGL4.71 载体特征

pGL4.71载体简介

pGL4.71载体简介
The pGL4.71[hRlucP] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.71[hRlucP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucP luciferase reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

pGL4.71载体序列

LOCUS       AY738227                3653 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Renilla luciferase reporter vector pGL4.71[hRlucP], complete
            sequence.
ACCESSION   AY738227
VERSION     AY738227.1  GI:55535633
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.71[hRlucP]
  ORGANISM  Renilla luciferase reporter vector pGL4.71[hRlucP]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Renilla luciferase reporter vector
                     pGL4.71[hRlucP]"
                     /mol_type="other DNA"
                     /db_xref="taxon:296664"
     misc_feature    1..70
                     /note="multiple cloning site region"
     CDS             100..1158
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRlucP"
                     /protein_id="AAV52879.1"
                     /db_xref="GI:55535634"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
                     ESGMDRHPAACASARINV"
     misc_feature    1198..1419
                     /note="SV40 late poly(A) region"
     primer_bind     1487..1506
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      1744
                     /note="ColE1-derived"
     gene            complement(2535..3395)
                     /gene="ampR"
     CDS             complement(2535..3395)
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52880.1"
                     /db_xref="GI:55535635"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3500..3653
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(3602..3621)
                     /note="reporter vector primer 3 (RVPrimer3)"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
     1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
     1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
     1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
     1441 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     1501 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     1561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
     2521 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
     2581 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
     2641 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
     2701 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
     2761 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
     2821 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
     2881 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
     2941 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
     3001 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
     3061 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
     3121 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
     3181 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
     3241 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
     3301 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
     3361 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
     3421 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
     3481 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     3541 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     3601 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//

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