出品公司: | Promega |
---|---|
载体名称: | pGL4.71 ; pGL4.71[hRlucP] |
质粒类型: | 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体 |
高拷贝/低拷贝: | -- |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | 无 |
载体大小: | 3653 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | hRlucP |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | TOP10等常规菌株 |
宿主细胞(系): | HEK293等 |
备注: | pGL4.71[hRlucP]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRlucP。 |
产品目录号: | E6891 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pGL4.71[hRlucP]
价格:1500元
联系方式:I47-825O-882O
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pGL4.71载体基本信息
pGL4.71载体质粒图谱和多克隆位点信息
pGL4.71载体简介
pGL4.71载体简介 The pGL4.71[hRlucP] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.71[hRlucP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucP luciferase reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
pGL4.71载体序列
LOCUS AY738227 3653 bp DNA circular SYN 08-NOV-2004 DEFINITION Renilla luciferase reporter vector pGL4.71[hRlucP], complete sequence. ACCESSION AY738227 VERSION AY738227.1 GI:55535633 KEYWORDS . SOURCE Renilla luciferase reporter vector pGL4.71[hRlucP] ORGANISM Renilla luciferase reporter vector pGL4.71[hRlucP] other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3653) AUTHORS Almond,B., Chauvin,F. and Browning,J. TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega Corporation JOURNAL Unpublished REFERENCE 2 (bases 1 to 3653) AUTHORS Almond,B., Chauvin,F. and Browning,J. TITLE Direct Submission JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA FEATURES Location/Qualifiers source 1..3653 /organism="Renilla luciferase reporter vector pGL4.71[hRlucP]" /mol_type="other DNA" /db_xref="taxon:296664" misc_feature 1..70 /note="multiple cloning site region" CDS 100..1158 /codon_start=1 /transl_table=11 /product="luciferase hRlucP" /protein_id="AAV52879.1" /db_xref="GI:55535634" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ ESGMDRHPAACASARINV" misc_feature 1198..1419 /note="SV40 late poly(A) region" primer_bind 1487..1506 /note="reporter vector primer 4 (RVPrimer4)" rep_origin 1744 /note="ColE1-derived" gene complement(2535..3395) /gene="ampR" CDS complement(2535..3395) /gene="ampR" /note="AmpR" /codon_start=1 /transl_table=11 /product="synthetic beta-lactamase" /protein_id="AAV52880.1" /db_xref="GI:55535635" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3500..3653 /regulatory_class="polyA_signal_sequence" /note="synthetic; transcriptional pause site" primer_bind complement(3602..3621) /note="reporter vector primer 3 (RVPrimer3)" ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc 61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac 121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg 181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg 241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc 301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc 361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg 421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt 481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg 541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag 601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca 661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag 721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag 781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc 841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc 901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc 961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg 1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc 1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc 1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag 1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga 1441 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 1501 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca 1561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 1621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 1681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 1741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 1801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 1861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 1921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 1981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 2041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 2101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 2161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 2221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 2281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 2341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 2401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 2461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta 2521 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta 2581 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc 2641 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat 2701 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg 2761 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg 2821 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc 2881 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata 2941 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc 3001 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc 3061 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata 3121 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt 3181 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg 3241 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct 3301 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag 3361 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga 3421 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg 3481 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt 3541 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa 3601 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct //
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pGL4.71载体应用举例
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