pTA7001-DEST

LOCUS       pTA7001-DEST              16023 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  GATEWAY compatible derivative of plant binary vector pTA7001 from 
            Nam Chua's laboratory at The Rockefeller University, Dexamethasone 
            inducible.
ACCESSION   .
VERSION     .
KEYWORDS    pTA7001-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16023)
  AUTHORS   Gu Y, Innes RW
  TITLE     The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED 
            DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome 
            vesicles.
  JOURNAL   Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. 
            Epub 2011 Feb 22.
  PUBMED    21343429
REFERENCE   2  (bases 1 to 16023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16023
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(178..828)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    1293..1317
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        1832..2177
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2120..2140
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2439..2672
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        3964..4143
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3996..4179
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      5294..5546
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5766..5812
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    5846..5970
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5995..6025
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6079..6738
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6145..6164)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7080..7385
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7264..7283
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7426..7550)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8092..8109)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8092..8108)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8101..8123)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8248..8267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8458..8479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8726..8750
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10346..11494)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11793..12587)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12779..13546
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     rep_origin      14169..14880
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15007..15378)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            15411..15520
                     /note="incP origin of transfer"
     primer_bind     15743..15762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15996..16013
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgccagaa ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg
       61 gcatgaaaaa gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg
      121 atgttgtcta catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca
      181 atcgtcaccc tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc
      241 atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc
      301 gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc
      361 gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat
      421 tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag
      481 ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc
      541 gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga
      601 gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc
      661 ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg
      721 aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa
      781 caggtccagg gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt
      841 atcactgata aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa
      901 tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacatacc cctgatcgta
      961 attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg
     1021 ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg
     1081 gcgctgtatg cgttggtgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt
     1141 ttcgggcggc ggccaatctt gctcgtctcg ctggccggcg ccagatctgg ggaaccctgt
     1201 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
     1261 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     1321 actgatagtt tcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
     1381 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
     1441 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
     1501 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
     1561 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
     1621 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
     1681 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
     1741 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
     1801 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     1861 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     1921 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1981 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     2041 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     2101 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     2161 ttcatttgga gagaacacgg gggactctag tttaaacaat ggcagatcca atgaagctac
     2221 tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag
     2281 aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa
     2341 ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac
     2401 tggaacagct atttctactg atttttccta ggtcgagcgc ccccccgacc gatgtcagcc
     2461 tgggggacga gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag
     2521 acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc
     2581 acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta
     2641 ccgatgccct tggaattgac gagtacggtg gggatccaat tcagcaagcc actgcaggag
     2701 tctcacaaga cacttcggaa aatcctaaca aaacaatagt tcctgctgca ttaccacagc
     2761 tcacccctac cttggtgtca ctgctggagg tgattgaacc cgaggtgttg tatgcaggat
     2821 atgatagctc tgttccagat tcagcatgga gaattatgac cacactcaac atgttaggtg
     2881 ggcgtcaagt gattgcagca gtgaaatggg caaaggcgat accaggcttc agaaacttac
     2941 acctggatga ccaaatgacc ctgctacagt actcatggat gtttctcatg gcatttgccc
     3001 tgggttggag atcatacaga caatcaagtg gaaacctgct ctgctttgct cctgatctga
     3061 ttattaatga gcagagaatg tctctaccct gcatgtatga ccaatgtaaa cacatgctgt
     3121 ttgtctcctc tgaattacaa agattgcagg tatcctatga agagtatctc tgtatgaaaa
     3181 ccttactgct tctctcctca gttcctaagg aaggtctgaa gagccaagag ttatttgatg
     3241 agattcgaat gacttatatc aaagagctag gaaaagccat cgtcaaaagg gaagggaact
     3301 ccagtcagaa ctggcaacgg ttttaccaac tgacaaagct tctggactcc atgcatgagg
     3361 tggttgagaa tctccttacc tactgcttcc agacattttt ggataagacc atgagtattg
     3421 aattcccaga gatgttagct gaaatcatca ctaatcagat accaaaatat tcaaatggaa
     3481 atatcaaaaa gcttctgttt catcaaaaat gactcgacct aactgagtaa gctagcttgt
     3541 tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
     3601 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
     3661 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
     3721 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
     3781 aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acactagatc
     3841 cccaaacaag cttgaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     3901 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     3961 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     4021 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     4081 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     4141 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatccgct agaggatctc
     4201 gacctgcaag atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct
     4261 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     4321 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     4381 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     4441 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     4501 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     4561 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     4621 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     4681 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     4741 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     4801 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     4861 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     4921 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     4981 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     5041 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     5101 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     5161 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     5221 cgacgcccca gcactcgtcc gggatcttgg aggtgatgta acatgatcac aagctgatcc
     5281 cccgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5341 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     5401 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     5461 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     5521 cgcggtgtca tctatgttac tagatcggga attgatcccc cctcgacagc ttgcatgccg
     5581 gtcgactcta gaggatccgg gtgacagccc tccgacgggt gacagccctc cgacgggtga
     5641 cagccctccg aattctagag gatccgggtg acagccctcc gacgggtgac agccctccga
     5701 cgggtgacag ccctccgaat tcgagctcgg tacccgggga tctgtcgacc tcgatcgaga
     5761 tcttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacacgctga
     5821 agctagtcga ctctagcctc gaatcacaag tttgtacaaa aaagctgaac gagaaacgta
     5881 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     5941 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     6001 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     6061 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     6121 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     6181 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     6241 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     6301 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     6361 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     6421 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     6481 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     6541 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     6601 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     6661 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     6721 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
     6781 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     6841 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     6901 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     6961 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     7021 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     7081 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     7141 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     7201 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     7261 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     7321 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     7381 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     7441 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     7501 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gattcgaggc
     7561 tagaggatcg actagtcgac tagtcgatcc aggcctccca gctttcgtcc gtatcatcgg
     7621 tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcccacacac cagaatccta
     7681 ctaagtttga gtattatggc attggaaaag ctgttttctt ctatcatttg ttctgcttgt
     7741 aatttactgt gttctttcag tttttgtttt cggacatcaa aatgcaaatg gatggataag
     7801 agttaataaa tgatatggtc cttttgttca ttctcaaatt attattatct gttgttttta
     7861 ctttaatggg ttgaatttaa gtaagaaagg aactaacagt gtgatattaa ggtgcaatgt
     7921 tagacatata aaacagtctt tcacctctct ttggttatgt cttgaattgg tttgtttctt
     7981 cacttatctg tgtaatcaag tttactatga gtctatgatc aagtaattat gcaatcaagt
     8041 taagtacagt ataggctttt tgtgtcgagg gggtaccgag tcgaggaatt cactggccgt
     8101 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8161 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8221 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8281 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8341 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     8401 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8461 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     8521 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
     8581 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     8641 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
     8701 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     8761 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     8821 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     8881 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     8941 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
     9001 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
     9061 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
     9121 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
     9181 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
     9241 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     9301 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     9361 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     9421 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     9481 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     9541 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     9601 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     9661 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     9721 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     9781 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     9841 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
     9901 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
     9961 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
    10021 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
    10081 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
    10141 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
    10201 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
    10261 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
    10321 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
    10381 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
    10441 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
    10501 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
    10561 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
    10621 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
    10681 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
    10741 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
    10801 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
    10861 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
    10921 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
    10981 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
    11041 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
    11101 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
    11161 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
    11221 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    11281 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    11341 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    11401 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    11461 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    11521 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    11581 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    11641 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    11701 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    11761 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    11821 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    11881 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    11941 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    12001 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    12061 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    12121 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    12181 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    12241 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    12301 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    12361 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    12421 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    12481 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    12541 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    12601 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    12661 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    12721 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    12781 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
    12841 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
    12901 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
    12961 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
    13021 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
    13081 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
    13141 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
    13201 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
    13261 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
    13321 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
    13381 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
    13441 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
    13501 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
    13561 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
    13621 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
    13681 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
    13741 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
    13801 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
    13861 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
    13921 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
    13981 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
    14041 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
    14101 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
    14161 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
    14221 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
    14281 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    14341 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    14401 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    14461 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    14521 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    14581 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    14641 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    14701 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    14761 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    14821 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
    14881 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
    14941 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
    15001 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
    15061 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
    15121 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
    15181 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
    15241 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
    15301 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
    15361 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
    15421 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
    15481 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
    15541 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
    15601 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
    15661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    15721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    15781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    15841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    15901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    15961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    16021 aag
//