pNTAP-Shuttle-C

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Agilent
载体名称: pNTAP-Shuttle-C
质粒类型: 腺病毒载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 7737 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: SBP; CBP (N-端)
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: DH5α
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 腺病毒载体pNTAP-Shuttle-C载体表达表达N端融合链霉亲和素肽和钙结合肽的目的蛋白。
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pNTAP-Shuttle-C载体图谱



pNTAP-Shuttle-C 多克隆位点

pNTAP-Shuttle-C 载体特征

载体简介

载体序列

pNTAP Shuttle C, 7737 bp                       version 016001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 


    1  CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
   51  GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
  101  TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
  151  GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
  201  GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
  251  CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
  301  AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
  351  GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
  401  TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  451  CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
  501  GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  551  TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
  601  AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
  651  GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
  701  TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  751  TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
  801  TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  851  CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
  901  AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CTAGCATGAA
  951  GCGACGATGG AAAAAGAATT TCATAGCCGT CTCAGCAGCC AACCGCTTTA
 1001  AGAAAATCTC ATCCTCCGGG GCACTTGGAA GCGGTAGCGG TACCATGGAC
 1051  GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
 1101  CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC
 1151  GGGAGCCCTC CGGCGGCTGC AAGCTGGGCT TGCCCGGGCG GATCCCCCGG
 1201  GCGTCGACGC GGCCGCTCGA GCCGAAGCTT ATCGATACGC GTCCGATCGG
 1251  ATATCCGATC CACCGGATCT AGATAACTGA TCATAATCAG CCATACCACA
 1301  TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA
 1351  CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG
 1401  CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
 1451  GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
 1501  ATCTTAACGC GGATCTGGGC GTGGTTAAGG GTGGGAAAGA ATATATAAGG
 1551  TGGGGGTCTT ATGTAGTTTT GTATCTGTTT TGCAGCAGCC GCCGCCGCCA
 1601  TGAGCACCAA CTCGTTTGAT GGAAGCATTG TGAGCTCATA TTTGACAACG
 1651  CGCATGCCCC CATGGGCCGG GGTGCGTCAG AATGTGATGG GCTCCAGCAT
 1701  TGATGGTCGC CCCGTCCTGC CCGCAAACTC TACTACCTTG ACCTACGAGA
 1751  CCGTGTCTGG AACGCCGTTG GAGACTGCAG CCTCCGCCGC CGCTTCAGCC
 1801  GCTGCAGCCA CCGCCCGCGG GATTGTGACT GACTTTGCTT TCCTGAGCCC
 1851  GCTTGCAAGC AGTGCAGCTT CCCGTTCATC CGCCCGCGAT GACAAGTTGA
 1901  CGGCTCTTTT GGCACAATTG GATTCTTTGA CCCGGGAACT TAATGTCGTT
 1951  TCTCAGCAGC TGTTGGATCT GCGCCAGCAG GTTTCTGCCC TGAAGGCTTC
 2001  CTCCCCTCCC AATGCGGTTT AAAACATAAA TAAAAAACCA GACTCTGTTT
 2051  GGATTTGGAT CAAGCAAGTG TCTTGCTGTC TTTATTTAGG GGTTTTGCGC
 2101  GCGCGGTAGG CCCGGGACCA GCGGTCTCGG TCGTTGAGGG TCCTGTGTAT
 2151  TTTTTCCAGG ACGTGGTAAA GGTGACTCTG GATGTTCAGA TACATGGGCA
 2201  TAAGCCCGTC TCTGGGGTGG AGGTAGCACC ACTGCAGAGC TTCATGCTGC
 2251  GGGGTGGTGT TGTAGATGAT CCAGTCGTAG CAGGAGCGCT GGGCGTGGTG
 2301  CCTAAAAATG TCTTTCAGTA GCAAGCTGAT TGCCAGGGGC AGGCCCTTGG
 2351  TGTAAGTGTT TACAAAGCGG TTAAGCTGGG ATGGGTGCAT ACGTGGGGAT
 2401  ATGAGATGCA TCTTGGACTG TATTTTTAGG TTGGCTATGT TCCCAGCCAT
 2451  ATCCCTCCGG GGATTCATGT TGTGCAGAAC CACCAGCACA GTGTATCCGG
 2501  TGCACTTGGG AAATTTGTCA TGTAGCTTAG AAGGAAATGC GTGGAAGAAC
 2551  TTGGAGACGC CCTTGTGACC TCCAAGATTT TCCATGCATT CGTCCATAAT
 2601  GATGGCAATG GGCCCACGGG CGGCGGCCTG GGCGAAGATA TTTCTGGGAT
 2651  CACTAACGTC ATAGTTGTGT TCCAGGATGA GATCGTCATA GGCCATTTTT
 2701  ACAAAGCGCG GGCGGAGGGT GCCAGACTGC GGTATAATGG TTCCATCCGG
 2751  CCCAGGGGCG TAGTTACCCT CACAGATTTG CATTTCCCAC GCTTTGAGTT
 2801  CAGATGGGGG GATCATGTCT ACCTGCGGGG CGATGAAGAA AACGGTTTCC
 2851  GGGGTAGGGG AGATCAGCTG GGAAGAAAGC AGGTTCCTGA GCAGCTGCGA
 2901  CTTACCGCAG CCGGTGGGCC CGTAAATCAC ACCTATTACC GGGTGCAACT
 2951  GGTAGTTAAG AGAGCTGCAG CTGCCGTCAT CCCTGAGCAG GGGGGCCACT
 3001  TCGTTAAGCA TGTCCCTGAC TCGCATGTTT TCCCTGACCA AATCCGCCAG
 3051  AAGGCGCTCG CCGCCCAGCG ATAGCAGTTC TTGCAAGGAA GCAAAGTTTT
 3101  TCAACGGTTT GAGACCGTCC GCCGTAGGCA TGCTTTTGAG CGTTTGACCA
 3151  AGCAGTTCCA GGCGGTCCCA CAGCTCGGTC ACCTGCTCTA CGGCATCTCG
 3201  ATCCAGCATA TCTCCTCGTT TCGCGGGTTG GGGCGGCTTT CGCTGTACGG
 3251  CAGTAGTCGG TGCTCGTCCA GACGGGCCAG GGTCATGTCT TTCCACGGGC
 3301  GCAGGGTCCT CGTCAGCGTA GTCTGGGTCA CGGTGAAGGG GTGCGCTCCG
 3351  GGCTGCGCGC TGGCCAGGGT GCGCTTGAGG CTGGTCCTGC TGGTGCTGAA
 3401  GCGCTGCCGG TCTTCGCCCT GCGCGTCGGC CAGGTAGCAT TTGACCATGG
 3451  TGTCATAGTC CAGCCCCTCC GCGGCGTGGC CCTTGGCGCG CAGCTTGCCC
 3501  TTGGAGGAGG CGCCGCACGA GGGGCAGTGC AGACTTTTGA GGGCGTAGAG
 3551  CTTGGGCGCG AGAAATACCG ATTCCGGGGA GTAGGCATCC GCGCCGCAGG
 3601  CCCCGCAGAC GGTCTCGCAT TCCACGAGCC AGGTGAGCTC TGGCCGTTCG
 3651  GGGTCAAAAA CCAGGTTTCC CCCATGCTTT TTGATGCGTT TCTTACCTCT
 3701  GGTTTCCATG AGCCGGTGTC CACGCTCGGT GACGAAAAGG CTGTCCGTGT
 3751  CCCCGTATAC AGACTTGAGA GGGAGTTTAA ACGAATTCAA TAGCTTGTTG
 3801  CATGGGCGGC GATATAAAAT GCAAGGTGCT GCTCAAAAAA TCAGGCAAAG
 3851  CCTCGCGCAA AAAAGAAAGC ACATCGTAGT CATGCTCATG CAGATAAAGG
 3901  CAGGTAAGCT CCGGAACCAC CACAGAAAAA GACACCATTT TTCTCTCAAA
 3951  CATGTCTGCG GGTTTCTGCA TAAACACAAA ATAAAATAAC AAAAAAACAT
 4001  TTAAACATTA GAAGCCTGTC TTACAACAGG AAAAACAACC CTTATAAGCA
 4051  TAAGACGGAC TACGGCCATG CCGGCGTGAC CGTAAAAAAA CTGGTCACCG
 4101  TGATTAAAAA GCACCACCGA CAGCTCCTCG GTCATGTCCG GAGTCATAAT
 4151  GTAAGACTCG GTAAACACAT CAGGTTGATT CACATCGGTC AGTGCTAAAA
 4201  AGCGACCGAA ATAGCCCGGG GGAATACATA CCCGCAGGCG TAGAGACAAC
 4251  ATTACAGCCC CCATAGGAGG TATAACAAAA TTAATAGGAG AGAAAAACAC
 4301  ATAAACACCT GAAAAACCCT CCTGCCTAGG CAAAATAGCA CCCTCCCGCT
 4351  CCAGAACAAC ATACAGCGCT TCCACAGCGG CAGCCATAAC AGTCAGCCTT
 4401  ACCAGTAAAA AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA
 4451  GCTCAATCAG TCACAGTGTA AAAAAGGGCC AAGTGCAGAG CGAGTATATA
 4501  TAGGACTAAA AAATGACGTA ACGGTTAAAG TCCACAAAAA ACACCCAGAA
 4551  AACCGCACGC GAACCTACGC CCAGAAACGA AAGCCAAAAA ACCCACAACT
 4601  TCCTCAAATC GTCACTTCCG TTTTCCCACG TTACGTCACT TCCCATTTTA
 4651  AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC TAAAACCTAC
 4701  GTCACCCGCC CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC
 4751  TCATTATCAT ATTGGCTTCA ATCCAAAATA AGGTATATTA TTGATGATGT
 4801  TAATTAACAT GCATGGATCC ATATGCGGTG TGAAATACCG CACAGATGCG
 4851  TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC
 4901  TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA
 4951  GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA
 5001  TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
 5051  CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
 5101  ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
 5151  CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
 5201  CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
 5251  TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
 5301  AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
 5351  TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
 5401  ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
 5451  GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG
 5501  ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
 5551  AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
 5601  TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
 5651  GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
 5701  ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
 5751  TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
 5801  TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
 5851  AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT
 5901  AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
 5951  ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
 6001  GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
 6051  CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
 6101  GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGCCA TGAGATTATC
 6151  AAAAAGGATC TTCACCTAGA TCCTTTTCAC GTAGAAAGCC AGTCCGCAGA
 6201  AACGGTGCTG ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG
 6251  GAAAACGCAA GCGCAAAGAG AAAGCAGGTA GCTTGCAGTG GGCTTACATG
 6301  GCGATAGCTA GACTGGGCGG TTTTATGGAC AGCAAGCGAA CCGGAATTGC
 6351  CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA AGTAAACTGG
 6401  ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGCTCTGA
 6451  TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
 6501  ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG
 6551  GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
 6601  GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
 6651  ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
 6701  GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
 6751  GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
 6801  CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT
 6851  ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
 6901  CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
 6951  TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC
 7001  AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC
 7051  CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
 7101  ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT
 7151  ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT
 7201  CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
 7251  GCCTTCTTGA CGAGTTCTTC TGAATTTTGT TAAAATTTTT GTTAAATCAG
 7301  CTCATTTTTT AACCAATAGG CCGAAATCGG CACCATCCCT TATAAATCAA
 7351  AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT
 7401  CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA
 7451  TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGT
 7501  GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA
 7551  TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
 7601  GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC
 7651  TGCGCGTAAC CACCACACCC GCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
 7701  CCATTCGCCA TTCAGGATCG AATTAATTCT TAATTAA

载体质粒图谱pdf版和相关资料下载

载体应用举例

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