RNAi-Ready pSIREN-RetroQ

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: RNAi-Ready pSIREN-RetroQ
质粒类型: 哺乳动物细胞自失活基因沉默逆病毒载体,shRNA
高拷贝/低拷贝: 低拷贝
启动子: Human U6
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6455 bp
5' 测序引物及序列: U6-F: ATGGACTATCATATGCTTACCGTA (Clontech )
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素 (Puromycin)
备注:  
产品目录号: 631526
稳定性: /
组成型: 诱导型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

RNAi-Ready pSIREN-RetroQ载体图谱



RNAi-Ready pSIREN-RetroQ多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

载体简介

载体序列

LOCUS       pSIREN-RetroQ   6455 bp    DNA   circular            13-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 631526
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    1..727
                     /label=5'-LTR\(CMV/MSV)
     misc_feature    757..1566
                     /label=Extended\packaging\signal
     misc_feature    3161..3586
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(5233..6093)
                     /label=Amp(R)
     misc_feature    3866..4152
                     /label=SV40\Promoter
     promoter        1582..1839
                     /label=Human\U6\promoter
     rep_origin      complement(4472..5071)
                     /label=pUC\origin
     promoter        1852..2360
                     /label=PGK\Promoter
     CDS             2381..2980
                     /label=Puro(R)
     rep_origin      4087..4152
                     /label=SV40\origin
     misc_feature    1834..1856
                     /label=shRNA\Cloning\Site
BASE COUNT     1458 a      1810 c      1668 g      1519 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc tgggcaggaa gagggcctat ttcccatgat tccttcatat 
     1621 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca 
     1681 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt 
     1741 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat 
     1801 ttcttggctt tatatatctt gtggaaagga cgaggatccg gacaagcttc gaattctacc 
     1861 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg 
     1921 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc 
     1981 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca 
     2041 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac 
     2101 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt 
     2161 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg 
     2221 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct 
     2281 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt 
     2341 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac 
     2401 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 
     2461 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 
     2521 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
     2581 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
     2641 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
     2701 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
     2761 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
     2821 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
     2881 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 
     2941 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca 
     3001 gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgagata tcagtggtcc 
     3061 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga 
     3121 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta 
     3181 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga 
     3241 gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc ctagagaacc 
     3301 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa 
     3361 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga 
     3421 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg 
     3481 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag 
     3541 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg 
     3601 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc 
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc 
     3841 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     3901 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 
     3961 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
     4021 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     4081 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 
     4141 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta 
     4201 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 
     4261 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 
     4321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     4381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     4441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     4501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     4561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     4621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     4681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     4741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     4801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     4861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
     4921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     4981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     5041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     5101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     5161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     5221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     5281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     5341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     5401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     5461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     5521 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 
     5581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     5641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     5701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     5761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     5821 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 
     5881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     5941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     6001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     6061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     6121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     6181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 
     6241 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga 
     6301 attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc 
     6361 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat 
     6421 tacggggtca ttagttcata gcccatatat ggagt 
//

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