| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141381 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 9954bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp15 |
| 物种来源: | SARS-CoV-2 |
| 插入大小: | 951 bp |
| 基因突变: | human codon optimized |
| GenBank编号 | JN247409 |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141381 ; http://n2t.net/addgene:141381 ; RRID:Addgene_141381) |
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro 9954 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9954)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9954)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9954
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 2272..2295
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 2332..2355
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 2367..2940
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(2534..2551)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2761..2780
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2973..3572
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2973..2992)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3469..3489
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 3586..4174
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3639..3659)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4057..4068)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4381..5014
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5142..5158)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5142..5158)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5155..5177)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5166..5182
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5190..5220)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5235..5256
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5373..5390)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5544..6132)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5624..5643)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6303..7163)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6926..6945
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7164..7268)
/gene="bla"
/label=AmpR promoter
polyA_signal 7316..7450
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7353..7372)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7407..7426
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 7618..8251
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 8298..8423
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8920..9153
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9338..9382
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 9645..9760
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatgagtct tgaaaacgtc gccttcaacg tagtaaataa gggccacttt
1261 gatggtcaac agggagaagt acccgtgtct atcatcaata acaccgttta cacgaaggtt
1321 gacggcgttg atgttgaact gttcgaaaat aagacgacac tcccggtgaa cgtagctttc
1381 gagctttggg cgaagcgaaa tataaaacct gtccctgaag ttaaaattct caataatctt
1441 ggggtagaca tcgcggctaa tacagtcata tgggactata agcgggacgc tccggcgcat
1501 atatctacca tcggagtgtg ctcaatgaca gatattgcca agaaacctac agagacgatt
1561 tgcgctcctc ttaccgtatt ttttgacggc agggtcgacg gacaagtgga tctgttccga
1621 aacgcgcgca atggtgttct catcactgag ggtagtgtaa agggactcca gcctagtgtc
1681 ggacctaagc aagcctcttt gaatggggtc actctgatcg gcgaggctgt taagacgcaa
1741 tttaactact ataagaaggt cgatggcgtc gtgcagcagc ttcctgaaac atactttaca
1801 cagtctcgca acttgcaaga atttaaaccg cgctcacaga tggaaataga ctttctcgaa
1861 ctggcaatgg acgagttcat agagcggtac aaattggaag ggtatgcctt tgaacacatt
1921 gtttatggtg acttttctca cagccagttg ggtggtttgc atctcctcat agggctggcc
1981 aaaaggttca aagagagccc ctttgaattg gaggacttca tacctatgga ttcaacagtt
2041 aagaattact tcattacgga tgcgcagaca ggcagtagta agtgtgtctg ctccgtaatt
2101 gacctcctct tggacgactt tgtagagata attaagtccc aggatcttag tgtcgtatcc
2161 aaggtcgtta aggtcacaat tgattacacc gagattagtt ttatgctgtg gtgtaaagac
2221 ggtcacgtcg agaccttcta tccaaagttg caactcgaag gcggcggggg atggagccat
2281 ccacaattcg agaaaggcgg tggttcagga ggaggtagcg ggggtggatc atggtcacat
2341 ccgcagtttg aaaagtaagg atcccgcccc tctccctccc ccccccctaa cgttactggc
2401 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
2461 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
2521 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
2581 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
2641 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
2701 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
2761 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
2821 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
2881 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
2941 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
3001 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
3061 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
3121 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
3181 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
3241 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
3301 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
3361 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
3421 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
3481 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
3541 cctggtgcat gacccgcaag cccggtgcct agacgcgtct ggaacaatca acctctggat
3601 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
3661 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
3721 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
3781 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
3841 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
3901 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
3961 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
4021 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
4081 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
4141 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
4201 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
4261 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
4321 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
4381 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
4441 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
4501 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
4561 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
4621 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
4681 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
4741 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
4801 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
4861 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
4921 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
4981 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
5041 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
5101 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
5161 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
5221 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
5281 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
5341 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
5401 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
5461 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
5521 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
5581 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
5641 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
5701 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
5761 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
5821 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
5881 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
5941 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
6001 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
6061 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
6121 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
6181 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
6241 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
6301 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
6361 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
6421 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
6481 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
6541 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
6601 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
6661 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
6721 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
6781 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
6841 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
6901 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
6961 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
7021 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
7081 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
7141 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
7201 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
7261 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
7321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
7381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
7441 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
7501 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
7561 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
7621 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
7681 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
7741 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
7801 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
7861 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
7921 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
7981 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
8041 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
8101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
8161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
8221 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
8281 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
8341 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
8401 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
8461 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
8521 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
8581 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
8641 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
8701 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
8761 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
8821 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
8881 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
8941 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
9001 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
9061 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
9121 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
9181 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
9241 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
9301 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
9361 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
9421 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
9481 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
9541 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
9601 agacagatcc attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg
9661 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
9721 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
9781 gcagagatcc agtttatcga tgagtaattc atacaaaagg actcgcccct gccttgggga
9841 atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg
9901 ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgag
//
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