| 载体名称: | pRU670 |
|---|---|
| 来源历史: | <- R. Schmitt |
| 质粒抗性: |
incT, Km抗性, Tc抗性, tra+, ts
|
| 载体说明: | 将转座子Tn1731（7.8kb）插入Kmr-ts结合质粒Rts1（Rts1：：Tn1731）中，Tn1731本身是Tn1722的Tcr变体，是Tn1722的体外重组体和携带Tn1721的tet基因的2.4kb EcoRI片段；EcoRI片段来自质粒pJOE452；可用于大肠杆菌的诱变；Rts1的大尺寸（217kb）阻止了任何Rts1：：Tn复制子的共转换。 |
| 宿主菌： | Escherichia coli K12 MM294 |
| 培养条件： | LB培养基，30度 |
| 参考文献： |
Altenbuchner, J., Schmid, K., Schmitt, R. (1983). Tn1721-encoded tetracycline resistance: mapping of structural and functional genes mediating resistance. J.Bacteriol. 153 : 116-123 . Heffron, F., (1983). Tn3 and its relatives. In Mobile Genetic Elements , pp. 223-260. Edited by J. A. Shapiro, Academic Press. Schmitt, R., Altenbuchner, J., Wiebauer, K., Arnold, W., Pühler, A., Schöffl, F. (1981). Basis of transposition and gene amplification by Tn1721 and related tetracycline resistance transposons. Cold Spring Harb.Symp.Quant.Biol. 45 : 59-65 . Terawaki, Y., Kobayashi, Y., Matsumoto, H., Kamio, Y. (1981). Molecular cloning and mapping of a deletion derivative of the plasmid Rts1. Plasmid 6 : 222-234 . Ubben, D., Schmitt, R. (1986). Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis. Gene 41 : 145-152 . Waters, S. H., Rogowsky, P., Grinsted, J., Altenbuchner, J., Schmitt, R. (1983). The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis. Nucl.Acids Res. 11 : 6089-6105 . |
| 安全等级： | 1级 |
| 分离国家: | 原产国未知 |
| 存储时间: | 1990年3月16日前 |
买家导航
