| 质粒名称: | pCHAC-mt-mKeima |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 72342 |
| 存储实验室: | Richard Youle |
|
质粒骨架基本信息 |
|
| 质粒名称: | pCHAC-MCS1-IRES-MCS2 |
| 质粒出品公司: | AlleleBiotechnology |
| 质粒抗性: | 氨苄霉素 |
| 空质粒大小: | 5632 bp |
| 完整质粒大小: | 6517 bp |
| 质粒类型: | 逆转录病毒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mt-mKeima |
| 物种来源: | stony coral Montipora |
| 插入大小: | 894 bp |
| GenBank编号 | AB209969 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI(未破坏) |
| 3' 克隆位点: | SalI (未破坏) |
| 5' 测序引物 | TGACCTGGGAAGCCTTGGCT |
| 3' 测序引物 | TTGCCAAAAGACGGCAATAT |
|
文献引用方法 |
|
| 材料和方法部分: | pCHAC-mt-mKeima was a gift from Richard Youle (Addgene plasmid # 72342) |
| 参考文献部分: | The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Lazarou M, Sliter DA, Kane LA, Sarraf SA, Wang C, Burman JL, Sideris DP, Fogel AI, Youle RJ. Nature. 2015 Aug 20;524(7565):309-14. doi: 10.1038/nature14893. Epub 2015 Aug 12. 10.1038/nature14893 PubMed 26266977 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCHAC-mt-mKeima 6517 bp DNA SYN
DEFINITION pCHAC-mt-mKeima
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6517
/organism="pCHAC-mt-mKeima"
/mol_type="other DNA"
misc_feature 431..1012
/label="3MoMuLV_LTR"
misc_feature 461..1012
/label="5_LTR2"
misc_feature 1012..2298
/label="psi_plus_pack2"
misc_feature 1488..1982
/label="gag"
CDS 1488..2162
/label="ORF frame 3"
misc_feature 1843..1865
/label="MSCV_primer"
misc_feature 1843..1865
/label="pLXSN_5_primer"
misc_feature 1881..1897
/label="pBABE_5_primer"
misc_feature 2234..2250
/label="pBMN_5_primer"
CDS 2311..3222
/label="ORF frame 1"
misc_feature 3229..3801
/label="IRES"
misc_feature 3924..4517
/label="3MoMuLV_LTR"
misc_feature 3966..4517
/label="5_LTR2"
gene complement(5581..6441)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(5581..6441)
/label="ORF frame 2"
ORIGIN
1 ATCACGAGGC CCTTTCGTCT TCAAGAACAG CTTTGCTCTT AGGAGTTTCC TAATACATCC
61 CAAACTCAAA TATATAAAGC ATTTGACTTG TTCTATGCCC TAGGGGGCGG GGGGAAGCTA
121 AGCCAGCTTT TTTTAACATT TAAAATGTTA ATTCCATTTT AAATGCACAG ATGTTTTTAT
181 TTCATAAGGG TTTCAATGTG CATGAATGCT GCAATATTCC TGTTACCAAA GCTAGTATAA
241 ATAAAAATAG ATAAACGTGG AAATTACTTA GAGTTTCTGT CATTAACGTT TCCTTCCTCA
301 GTTGACAACA TAAATGCGCT GCTGAGAAGC CAGTTTGCAT CTGTCAGGAT CAATTTCCCA
361 TTATGCCAGT CATATTAATT ACTAGTCAAT TAGTTGATTT TTATTTTTGA CATATACATG
421 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
481 GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA GATGGAACAG
541 CTGAATATGG GCCAAAGCGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA
601 GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC
661 CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG CAGTTTCTAG
721 AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG
781 AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT
841 AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA GTCGCCCGGG
901 TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT CGCTGTTCCT
961 TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA TTTGGGGGCT
1021 CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG AGGTAAGCTG
1081 GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT TTATGCGCCT
1141 GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG GAACTGACGA
1201 GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG GCCGTTTTTG
1261 TGGCCCGACC TGAGTCCAAA AATCCCGATC GTTTTGGACT CTTTGGTGCA CCCCCCTTAG
1321 AGGAGGGATA TGTGGTTCTG GTAGGAGACG AGAACCTAAA ACAGTTCCCG CCTCCGTCTG
1381 AATTTTTGCT TTCGGTTTGG GACCGAAGCC GCGCCGCGCG TCTTGTCTGC TGCAGCATCG
1441 TTCTGTGTTG TCTCTGTCTG ACTGTGTTTC TGTATTTGTC TGAAAATATG GGCCCGGGCC
1501 AGACTGTTAC CACTCCCTTA AGTTTGACCT TAGGTCACTG GAAAGATGTC GAGCGGATCG
1561 CTCACAACCA GTCGGTAGAT GTCAAGAAGA GACGTTGGGT TACCTTCTGC TCTGCAGAAT
1621 GGCCAACCTT TAACGTCGGA TGGCCGCGAG ACGGCACCTT TAACCGAGAC CTCATCACCC
1681 AGGTTAAGAT CAAGGTCTTT TCACCTGGCC CGCATGGACA CCCAGACCAG GTCCCCTACA
1741 TCGTGACCTG GGAAGCCTTG GCTTTTGACC CCCCTCCCTG GGTCAAGCCC TTTGTACACC
1801 CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT
1861 CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC TTCTCTAGGC GCCCCCATAT
1921 GGCCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT AAACTTCCCT GACCCTGACA
1981 TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT ACAGGCTCTC TACTTAGTCC
2041 AGCACGAAGT CTGGAGACCT CTGGCGGCAC GTACCAAGAA CAACTGGACC GACCGGTGGT
2101 ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA CTAAGAACCT
2161 AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG CCCTCAAAGT
2221 AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC CCGGGGGTGG
2281 ACCATCCTCT AGACTGCCGG ATCCACGCGT ATGCATCTCG AGGCCACCAT GTCCGTCCTG
2341 ACGCCGCTGC TGCTGCGGGG CTTGACAGGC TCGGCCCGGC GGCTCCCAGT GCCGCGCGCC
2401 AAGATCCATT CGTTGCCGCC GGAGGGGAAG CTTGGGATGT CCGTCCTGAC GCCGCTGCTG
2461 CTGCGGGGCT TGACAGGCTC GGCCCGGCGG CTCCCAGTGC CGCGCGCCAA GATCCATTCG
2521 TTGCCGCCGG AGGGGAAGCT CGGGggatcc GCCATGGTGA GCGTGATCGC CAAGCAGATG
2581 ACCTACAAGG TGTACATGAG CGGCACCGTG AACGGCCACT ACTTCGAGGT GGAGGGCGAC
2641 GGCAAGGGCA AGCCCTACGA GGGCGAGCAG ACCGTGAAGC TGACCGTGAC CAAGGGTGGC
2701 CCCCTGCCCT TCGCCTGGGA CATCCTGAGC CCCCAGCTCC AGTACGGCAG CATCCCCTTC
2761 ACCAAGTACC CCGAGGACAT CCCCGACTAC TTCAAGCAGA GCTTCCCCGA GGGCTACACC
2821 TGGGAGCGCA GCATGAACTT CGAGGACGGC GCCGTGTGCA CCGTGAGCAA CGACAGCAGC
2881 ATCCAGGGCA ACTGCTTCAT CTACAACGTG AAGATCAGCG GCGAGAACTT CCCCCCCAAC
2941 GGCCCCGTGA TGCAGAAGAA GACCCAGGGC TGGGAGCCCA GCACCGAGCG CCTGTTCGCC
3001 CGCGACGGAA TGCTGATCGG CAACGACTAC ATGGCCCTGA AGCTGGAGGG CGGCGGCCAC
3061 TACCTGTGCG AGTTCAAGAG CACCTACAAG GCCAAGAAGC CCGTGAGGAT GCCCGGCCGC
3121 CACGAGATCG ACCGCAAGCT GGACGTGACC AGCCACAACC GCGACTACAC CAGCGTGGAG
3181 CAGTGCGAGA TCGCCATCGC CCGCCACTCC CTGCTGGGCT AAGTCGACCC CCCCCCCCTA
3241 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT
3301 CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA
3361 CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG
3421 TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT
3481 GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT
3541 AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGG
3601 AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT GCCCAGAAGG
3661 TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC ATGCTTTACA TGTGTTTAGT
3721 CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA
3781 CACGATGATA ATATGGCCAC AACCATGGGG CGCGCCGTAT ACTGATCAAT CGATTCAATT
3841 GCACCGGTGC CTGCAGGCCA TCCCCTTGGC GTCTCCTCGA CGATAAAATA AAAGATTTTA
3901 TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT
3961 TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA
4021 TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
4081 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG
4141 ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG
4201 CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC
4261 CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG
4321 CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT
4381 CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA
4441 TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC
4501 AGCGGGGGTC TTTCATTCAC ATGCAGCATG TATCAAAATT AATTTGGTTT TTTTTCTTAA
4561 GTATTTACAT TAAATGGCCA TAGTTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
4621 GTTTGCGTAT TGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
4681 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
4741 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
4801 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
4861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
4921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
4981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
5041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
5101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
5161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
5221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
5281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
5341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
5401 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
5461 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
5521 TTAAAAATGA AGTTTGCGCA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
5581 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
5641 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
5701 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
5761 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
5821 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
5881 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
5941 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
6001 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
6061 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
6121 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
6181 CTCTTGCCCG GCGTCAACAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
6241 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
6301 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
6361 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
6421 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
6481 TTATTGTCTC ATGACATTAA CCTATAAAAA TAGGCGT
//
