pCCl-MCS_miniCMV_EGFP

LOCUS       pCCl-MCS_miniCMV_EGFP                9287 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  The plasmid includes a EGFP gene driven by a minimal CMV promoter. 
            Cis-regulatory elements can be cloned into the single EcoRV site. 
            This plasmid can be used for generating a lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    pCCl-MCS_miniCMV_EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  TITLE     A versatile reporter system for multiplexed screenings of effective 
            epigenome editors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             599..779
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    826..951
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1444..1677
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1862..1906
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2173..2290
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2427..2561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2661..3380)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(2664..3380)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2706..2727)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3055..3074
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3314..3335
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3457..3481)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3478..3516)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        3544..4054
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3973..3991
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5058..5646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5111..5131)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5529..5540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5733..5966
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6038..6159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6129..6148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6199..6334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6261..6280
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6354..6373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6355..6373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6383..6400)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6383..6399)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6392..6414)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6541..6996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6628..6647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6838..6859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7022..7126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7127..7987
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7345..7364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8158..8746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8647..8666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8900..8917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9034..9055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9070..9100
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9108..9124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9113..9135
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9132..9148
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9132..9148
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9167..9187
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9169..9187
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg
      601 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      661 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      721 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      781 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
      841 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
      901 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
      961 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     1021 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1081 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1141 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1201 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1261 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     1321 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1381 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1441 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1501 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1561 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1621 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1681 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1741 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1801 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1861 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1921 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1981 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2041 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2101 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     2161 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2221 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2281 tcaaaatttt atcgatcacg agactagcct cgatagatct gatcataatc agccatacca
     2341 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2401 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2461 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2521 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2581 cgcctgtcat cgaattcctg cagcccggtg catgactaag ctagctcagt tagcctcccc
     2641 catctctcga cgcggccgct ttacttgtac agctcgtcca tgccgagagt gatcccggcg
     2701 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     2761 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     2821 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     2881 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     2941 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     3001 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     3061 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     3121 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
     3181 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     3241 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     3301 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     3361 tcctcgccct tgctcaccat ggtgaattcc gcggaggctg gatcggtccc ggtgtcttct
     3421 atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc
     3481 tctgcttata taggcctccc accgtacacg cctaccctcg agaagcttga tatcgaattc
     3541 cacggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     3601 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     3661 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     3721 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     3781 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     3841 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     3901 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     3961 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     4021 cctcgttgac cgaatcaccg acctctctcc ccagggggat cccccgggct gcaggaattc
     4081 gggccgcggc cagctccggc gggcaggggg ggcgctggag cgcagcgcag cgcagcccca
     4141 tcagtccgca aagcggaccg agctggaagt cgagcgctgc cgcgggaggc gggcgatggg
     4201 ggcaggtgcc accggccgcg ccatggacgg gccgcgcctg ctgctgttgc tgcttctggg
     4261 ggtgtccctt ggaggtgcca aggaggcatg ccccacaggc ctgtacacac acagcggtga
     4321 gtgctgcaaa gcctgcaacc tgggcgaggg tgtggcccag ccttgtggag ccaaccagac
     4381 cgtgtgtgag ccctgcctgg acagcgtgac gttctccgac gtggtgagcg cgaccgagcc
     4441 gtgcaagccg tgcaccgagt gcgtggggct ccagagcatg tcggcgccgt gcgtggaggc
     4501 cgacgacgcc gtgtgccgct gcgcctacgg ctactaccag gatgagacga ctgggcgctg
     4561 cgaggcgtgc cgcgtgtgcg aggcgggctc gggcctcgtg ttctcctgcc aggacaagca
     4621 gaacaccgtg tgcgaggagt gccccgacgg cacgtattcc gacgaggcca accacgtgga
     4681 cccgtgcctg ccctgcaccg tgtgcgagga caccgagcgc cagctccgcg agtgcacacg
     4741 ctgggccgac gccgagtgcg aggagatccc tggccgttgg attacacggt ccacaccccc
     4801 agagggctcg gacagcacag cccccagcac ccaggagcct gaggcacctc cagaacaaga
     4861 cctcatagcc agcacggtgg caggtgtggt gaccacagtg atgggcagct cccagcccgt
     4921 ggtgacccga ggcaccaccg acaacctcat ccctgtctat tgctccatcc tggctgctgt
     4981 ggttgtgggc cttgtggcct acatagcctt caagaggtgg aacaggggga tcctctagag
     5041 tcgagtctag agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5101 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5161 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5221 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5281 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5341 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5401 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5461 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5521 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5581 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5641 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5701 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5761 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5821 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5881 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5941 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6001 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6061 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6121 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6181 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6301 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6361 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6421 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6481 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6541 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6601 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6661 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6721 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6781 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6841 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6901 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6961 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7021 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7081 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7141 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7201 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7261 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7321 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7381 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7441 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7501 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7561 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7621 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7681 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7741 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7801 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7861 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7921 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7981 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8041 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8101 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8281 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8881 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8941 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9001 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9061 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9121 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9181 ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt atccatatca
     9241 taatatgtac atttatattg gctcatgtcc aacattaccg ccatgtt
//