| 质粒名称: | pBbdCas9S_arr2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 149639 |
| 存储实验室: | Christine Jacobs-Wagner |
|
载体骨架基本信息 |
|
| 载体名称: | pBbdCas9S |
| 载体出品公司: | CJW lab |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | dCas9, lacI, arr2 |
| 载体类型: | 细菌表达,CRISPR |
| 筛选标记: | 利福平 |
| 载体抗性: | 大观霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB 5-alpha F' Iq |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | PflaB, PpQE30 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBbdCas9S_arr2 was a gift from Christine Jacobs-Wagner (Addgene plasmid # 149639 ; http://n2t.net/addgene:149639 ; RRID:Addgene_149639) |
| 参考文献部分: | A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. 10.1128/AEM.02519-20 PubMed 33257311 |
买家导航
pBbdCas9S_arr2载体 pBbdCas9Sarr2质粒基本信息
pBbdCas9S_arr2质粒图谱 pBbdCas9Sarr2载体图谱和pBbdCas9S_arr2载体序列 pBbdCas9Sarr2质粒序列多克隆位点信息
pBbdCas9S_arr2质粒 pBbdCas9Sarr2载体简介
pBbdCas9S_arr2质粒序列 pBbdCas9Sarr2载体序列
LOCUS pBbdCas9S_arr2 11315 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
gRNA cloning.
ACCESSION .
VERSION .
KEYWORDS pBbdCas9S_arr2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11315)
AUTHORS Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J,
Jacobs-Wagner C
TITLE A CRISPR interference platform for selective downregulation of gene
expression in Borrelia burgdorferi.
JOURNAL Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi:
10.1128/AEM.02519-20.
PUBMED 33257311
REFERENCE 2 (bases 1 to 11315)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11315
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1603..1647
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 1623..1639
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 1655..1671
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 1690..1701
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1708..5814
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 5984..6775
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 6898..7486
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7387..7406
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 aaaaatttcc tggtaatcca acacaatctt atagaacttg tgaaccttta agaattgttg
61 gtgttgttga agattgggaa ggacatcctg ttgaattaat aagaggaatg ttagattcat
121 tagaagattt aaaacgtcgt ggtttacatg ttattgaaga ttaacctggc gcgccaaata
181 atgcccgtgt ccctgttgaa tagggacacg gttttttaac aattttttta ttcctaggcc
241 ttagtggccg gtacccgggg atccttatta ctggccgctt tctagcctgg atacttgtct
301 ggctagttgc attagagagt cagctagagc cctagggcta gctgtttgtg tattcggagc
361 cagtgtagtt tttcttttta ctaacgatac gggtaataat tgattgcctt ttacagcctg
421 accttgagac aattgcaaaa gtctgtccac agaggtttga cctaataatc taaaatcttg
481 tttgatagta gtaagtggag gtatgtagca actagaatct tctgtatcat cataacccac
541 aactgaaata tcagccccaa ctctaagtcc actttcagta atagctcgca tagctcctaa
601 agccatttga tcattagcaa ctaacatagc agtagggaca atgccttcat ttaacatttg
661 cattgtttgc tgaaatcctg acatagcaga ccaatctcct tctctttctg ctattggttg
721 tatttgattt cttgttaaat acttgtgcca tcctgctaat ctcaatctgg cacttactga
781 acttaaggga cctgcaagta gtgcaatttg ttgatggcct aatgcaacaa ggtgttcaac
841 tcctaacctt gttccatctt cgtgagaaaa aattatagag tttattgggg tttggtccga
901 cacatccaaa aacaatgctg gaacattagt acatgctgct tcaactgcaa tcgcatcttg
961 gtcatcaaga ggataattaa ttataagtcc actaacacgt tgagctaata aattatgtac
1021 tgctgcctta caagcctcta cacctgatct ctcaaccatt gatacaacaa cgcttgcacc
1081 aagttgatca gctctcgatt taattgcggc tacaatttga ctaggcgcat gtaatgcaag
1141 tgatgatgtg gcaactccaa ttaaaagtga ttgtttcccc gcaagttgct gagcaacgcg
1201 atttggtata taattcaact ctgccatagc tgcttcaacc ttctctcttg tttttgcaga
1261 tacatgactt gcttggttca caactctcga aaccgtttgg tagctaaccc cggcatattc
1321 agcaacatca tacaaagtaa caggtttcat ggatcattcc tccatgataa aatttaaatt
1381 tctgactttg gcaaatcctt ttgcctgtta aaaagaatag cacaaaaatt aaaaaaaaag
1441 aaacgcaaat agcaatagca ttaaaaataa ttattattat ttatttcttt gattaaaatc
1501 aaaagccttt aatgtatttt cttgatcttt catgcgctta acggtaaaat ccaaggtatt
1561 aagttcacct tccacttttc tctctctatc ttctctagaa aatcataaaa aatttatttg
1621 ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca atttcacaca
1681 gaattcatta aagaggagaa attacatatg gataagaaat actcaatagg cttagctatc
1741 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
1801 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
1861 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
1921 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
1981 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
2041 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
2101 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
2161 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
2221 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
2281 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
2341 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
2401 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
2461 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
2521 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
2581 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
2641 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
2701 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
2761 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa
2821 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg
2881 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc
2941 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca
3001 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat
3061 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa
3121 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt
3181 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat
3241 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact
3301 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta
3361 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa
3421 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta
3481 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa
3541 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg
3601 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt
3661 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg
3721 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc
3781 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca
3841 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct
3901 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg
3961 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa
4021 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta
4081 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc
4141 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat
4201 cgtttaagtg attatgatgt cgatgccatt gttccacaaa gtttccttaa agacgattca
4261 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca
4321 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta
4381 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt
4441 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg
4501 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga
4561 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa
4621 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc
4681 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt
4741 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa
4801 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca
4861 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa
4921 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa
4981 gtcaatattg tcaagaaaac agaagtccag acaggcggat tctccaagga gtcaatttta
5041 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat
5101 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
5161 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tctggaaaga
5221 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
5281 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
5341 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa
5401 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat
5461 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag
5521 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt
5581 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat
5641 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt
5701 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc
5761 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga ctaataaaag
5821 cttgatcaga tctcagcttt tttttgaagt gcctggcagt aagttgaaaa aatttgaaat
5881 aaacttggac tatgttaata aaaaaataca aattaacgaa caaaaagatt tgaattatta
5941 tataatttag ttaaaagcaa ttttaaatga gggaggtttc catatgaggg aagcggtgat
6001 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
6061 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
6121 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
6181 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
6241 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
6301 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
6361 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
6421 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
6481 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
6541 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
6601 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
6661 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
6721 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgacg
6781 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcatgacca aaatccctta
6841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
7021 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
7081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
7141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
7201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
7261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
7321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
7381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
7441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
7501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcact
7561 agatccccgg caagttcatc cttagggttt tcatatctct taaaaagtca atttctgctt
7621 gattaatttc ttgtggatta ttgtttttgc ggtttttcat tttttatctc cttgagtatt
7681 gaattttttc ttatatataa atatatagca aaaactattt ttgccaactt ttttacaaaa
7741 aattttttat taaaacactt agggctttgc taaattctct tttaaaagaa cttagtaaag
7801 ccctatggat ttaagaactg attatactta cgtagtaaaa atactacaga tattaatagt
7861 atagaccata tcaaaatcat aatcaattta tatttatcta aaattgtagt ccatacaaaa
7921 tacaaaaaaa ttaggttttt ctaaattcta ttttaaaaga acttagtaac acctaatctc
7981 taaattactt acaaaattct aaaattagat ttttagttct tcatatttct ttaaaagttt
8041 tttaagaaaa tctttttgat tttcaaaaat ttcatgcatc ataaaactag taaatttgga
8101 tttttttttg taaaaatcat aactttcttg ggttttaagt tgaaatctta atggttttat
8161 tggattttgc ctagattttt ttaacgccgg actttcttta tcttttataa aattataaga
8221 atttttaata ccattttcta ttaaataatc ttcttttata agcccctctt gcagtgcagt
8281 tgctaatctt aaatacttat acgcttgagt ttttgctaca tcaaagtctt ttataaaatc
8341 aagaaaagtt ttataaccat caaatttata atattgatta tcttttattt cttttaaaat
8401 tttaatattt tccaccctgt ggaatatttc ttttttgaaa ttgtcattta acttttcctt
8461 tagtgtcttg taataagaaa ttttactttc attaatcatt aaattttgtt caacataagt
8521 atctatcttt tttgttaaaa ctttaacaac acgttttttt tccataaaaa ttttccttta
8581 tttttgcaac attttcctta attgatataa ttgttactat tttagatttt ttaatatcca
8641 cttaagaatt aatccctcac tagaatattt tttattatag aattcatata catgtttttc
8701 taaatctata aatttatcaa caattgcttt attatagggt gtatctatct tttcttttct
8761 taataatcgt ctcatattct ttaaataaca acaaacactg ccggttttaa acctaaattc
8821 tatgtaatat atttttgaaa aagtatatac attttcaacc ccgtttattt catactttac
8881 aaaaacatta tttattggtt ttctataacc atagtaaatg cctaaaaatt tgtccccgtc
8941 tttcatagga tatagataaa gtttttgaat ctctgattta tctaataatt tttcaaaaaa
9001 aattatacac cgcttttgtt tgttactaac tactctatgc aatgccttta atatttttgt
9061 gtgatatata tttttatcac cttttttttc tttcagcagg aagatacttt cttttggaaa
9121 tttaattttt tgttcattta ttcttgcagc cttaagtccg attttcatat ttaatcctta
9181 tatagccttt ctatttaatt cttttaaact caataaactt ttttggtttt tcattagttc
9241 taataattcg caataatatg tgcaaaacac tttattgtat tctattttct tttggttatt
9301 caaatattct tttataattg gctttagaag ttcaatattt acttcttttt ttagttgttc
9361 aataaggata ttgaaaatgt ttgtttttaa atcttgataa ttttgttgag aattttcttt
9421 ttttctttca attgacttct ctaatttacg ctttatattg tttaaatcgc tatatttatg
9481 actttcaata ataaaatgag gcttaaattt gtaattttcg tatatttttt ggaaacttgt
9541 ttctaattgt ttagaattat attcattttt ttcaaattct ttttgagtgt tgcatagaat
9601 ttcctttaat ttttcttgtt tttctttcat acaagattta gtaaaatgta tatttttatt
9661 ttttagtaat ttaatttcta ttatttttat gattttaagt attttgatta tattatcttt
9721 atcaatattt aaattgaata aaattgtaag aaaaattgaa agaatttttt tacataaaaa
9781 gttacattta ttgaagtaat ttcttacttg atacttttct atttcattaa tctttctttc
9841 ttcttttata ttatttttat tatttaaaca ctcctttaaa tctacactcc catttttagt
9901 aaatctatct ttaaggcccc ttgaaactct attttgaaat cttaaatctt ttttttcttg
9961 aaagtattta tagatttttt ggtaacattc tttttttgaa aaattaagtt gatagtatat
10021 ttcggtcccc aaatttaccc ctaagtgttt gtgataatta gttgtgactt taaattcttt
10081 ttctaatttg taaaggtaat tttgcagtgt ttttagttta acgggtgttt gactatttct
10141 tttaaggttt tcattaaagt aatagagtat gtttttttgt gtgtatttct tatatgtttt
10201 atttatgtac gctagtgttg agataaggac tactaattta tactgtaatt tattgtagca
10261 agttgtggtt ttactggttt tgtttatatt agataagttt tccatttata gctccttgaa
10321 taccggattt tttatctata aagtatatat atcacaattt tgatttaaaa gtaaattctt
10381 tttttaaaaa tacaaaaatt ttttataaat tatatagacc atgatattgt attaatacta
10441 attgatttta atttaaggtt gaactaaaat aaacatagtt ttgtagaaaa taatttttca
10501 ttattttgct acttgatatt ggatcgtaaa aatattaggg ctttacaaag ttcttttaaa
10561 agagaattta gtaaagccct aagtctttta ataaaaaatt ttttgtaaaa aagttggcaa
10621 aaatagtttt tgctatatat ttatatataa gaaaaaattc aatactcaag gagataaaaa
10681 atggaaaact taaacaataa tccacaaaaa attaatcaaa taaataagca aactaatttt
10741 attggttttg aaaattttat agaacttgat aatcaagtta atcaaactaa agaaaaaagt
10801 aaattaagta agataggtaa gaaattaccc ggaataagca gtcaagtcta gtgcggccgc
10861 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
10921 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
10981 ggcagtcgcc ctaaaacaaa gttaaacatc atgaggaggt aacgtcatgg taaaagattg
11041 gattcctatt tctcatgata attataaaca agtacaagga ccattttatc atggaactaa
11101 agctaattta gcaattggtg atttactaac tacaggattt atttctcatt ttgaagatgg
11161 tcgaattctt aaacatattt atttttcagc tttaatggaa ccagcagttt ggggagctga
11221 acttgctatg tcactatctg gtcttgaagg tcgtggttat atatatatag ttgaaccaac
11281 aggaccattt gaagatgatc caaatcttac aaata
//
