| 质粒名称: | pAAV-CamKII-ArchT-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37807 |
| 存储实验室: | Edward Boyden |
|
载体骨架基本信息 |
|
| 载体名称: | AAV with CaMKII promoter |
| 载体出品公司: | Scott Sternson |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5088 bp |
| 完整质粒大小: | 6564 bp |
| 载体类型: | AAV ; Adeno-associated virus，哺乳细胞表达 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ArchT-GFP |
| 物种来源: | H. strain TP009 |
| 插入大小: | 1476 bp |
| 基因又名: | archaerhodopsin;archaerhodopsin TP009;ArchT |
| 基因突变: | N/A |
| 启动子: | CamKII |
| 标签/融合蛋白: | GFP (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | gaggctgtgagcagccacag |
| 3' 测序引物 | aaagagacagcaaccagg |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-CamKII-ArchT-GFP was a gift from Edward Boyden (爱迪基因 plasmid # 37807) |
| 参考文献部分: | A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, Baratta MV, Winkle J, Desimone R, Boyden ES. Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. 10.3389/fnsys.2011.00018 PubMed 21811444 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-CamKII-ArchT-GFP 6564 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-CamKII-ArchT-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6564)
AUTHORS Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A,
Baratta MV, Winkle J, Desimone R, Boyden ES
TITLE A high-light sensitivity optical neural silencer: development and
application to optogenetic control of non-human primate cortex.
JOURNAL Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.
PUBMED 21811444
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-CamKII-ArchT-GFP|
FEATURES Location/Qualifiers
source 1..6564
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(11..28)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(262..281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1564..1583
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2069..2090)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2281..2300
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 2459..2588
/label=AAV2 ITR
regulatory 3960..3969
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4725..5441
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(4767..4788)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5028..5047)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5375..5396
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5466..6054
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5519..5539)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5937..5948)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 6097..6218
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6108..6127)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 6162..6181
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
repeat_region 6380..6509
/label=AAV2 ITR
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa cattatggcc ttaggtcact tcatctccat ggggttcttc
2701 ttctgatttt ctagaaaatg agatgggggt gcagagagct tcctcagtga cctgcccagg
2761 gtcacatcag aaatgtcaga gctagaactt gaactcagat tactaatctt aaattccatg
2821 ccttgggggc atgcaagtac gatatacaga aggagtgaac tcattagggc agatgaccaa
2881 tgagtttagg aaagaagagt ccagggcagg gtacatctac accacccgcc cagccctggg
2941 tgagtccagc cacgttcacc tcattatagt tgcctctctc cagtcctacc ttgacgggaa
3001 gcacaagcag aaactgggac aggagcccca ggagaccaaa tcttcatggt ccctctggga
3061 ggatgggtgg ggagagctgt ggcagaggcc tcaggagggg ccctgctgct cagtggtgac
3121 agataggggt gagaaagcag acagagtcat tccgtcagca ttctgggtct gtttggtact
3181 tcttctcacg ctaaggtggc ggtgtgatat gcacaatggc taaaaagcag ggagagctgg
3241 aaagaaacaa ggacagagac agaggccaag tcaaccagac caattcccag aggaagcaaa
3301 gaaaccatta cagagactac aagggggaag ggaaggagag atgaattagc ttcccctgta
3361 aaccttagaa cccagctgtt gccagggcaa cggggcaata cctgtctctt cagaggagat
3421 gaagttgcca gggtaactac atcctgtctt tctcaaggac catcccagaa tgtggcaccc
3481 actagccgtt accatagcaa ctgcctcttt gccccactta atcccatccc gtctgttaaa
3541 agggccctat agttggaggt gggggaggta ggaagagcga tgatcacttg tggactaagt
3601 ttgttcacat ccccttctcc aaccccctca gtacatcacc ctgggagaac aaggtccact
3661 tgcttctggg cccacacagt cctgcagtat tgtgtatata aggccagggc aacggaggag
3721 caggttttga agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
3781 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
3841 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt caatcaagct ggttctccat
3901 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg
3961 ccaccatgga ccccatcgct ctgcaggcag gatacgacct gctgggcgac ggaaggccag
4021 agaccctgtg gctgggaatc ggaaccctgc tgatgctgat cggcaccttc tacttcatcg
4081 tgaagggctg gggcgtgacc gacaaggagg ccagggagta ctacagcatc acaatcctgg
4141 tgcccggcat cgccagcgcc gcctacctga gcatgttctt cggcatcggc ctgaccgagg
4201 tgaccgtggc cggcgaggtg ctggacatct actacgccag atacgccgac tggctgttca
4261 ccaccccttt gcttctgctc gacctggccc tgctggctaa ggtggacagg gtgagcatcg
4321 gaaccctggt gggagtggac gccctgatga tcgtgaccgg cctgatcggc gccctgagcc
4381 acaccccact ggctaggtac agctggtggc tgttcagcac catctgcatg atcgtggtgc
4441 tgtacttcct ggctaccagc ctgagggctg ctgctaagga gaggggacca gaggtggcta
4501 gcaccttcaa caccctgacc gccctggtgc tggtgctgtg gaccgcctac cccatcctgt
4561 ggatcatcgg aaccgaggga gctggagtgg tgggactggg aatcgagacc ctgctgttca
4621 tggtgctgga cgtgaccgcc aaagtgggct tcggcttcat cctgctgagg agcagggcca
4681 tcctgggcga caccgaggcc cccgagcccc gaccggtagt agcagtgagc aagggcgagg
4741 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
4801 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
4861 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
4921 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
4981 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
5041 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
5101 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
5161 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
5221 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
5281 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
5341 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
5401 ccgccgggat cactctcggc atggacgagc tgtacaagta agaattcgat atcaagctta
5461 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
5521 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
5581 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
5641 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
5701 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
5761 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
5821 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
5881 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
5941 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
6001 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
6061 accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac
6121 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
6181 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
6241 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
6301 aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc
6361 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
6421 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
6481 gcggcctcag tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa cgcgcgggga
6541 gaggcggttt gcgtattggg cgct
//
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