| 质粒名称: | PVS10 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104398 |
| 存储实验室: | Irina Artsimovitch |
|
载体骨架基本信息 |
|
| 载体名称: | pET |
| 载体出品公司: | Novagen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 15205 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | rpoA-rpoB-rpoC-6xHis and rpoZ |
| 物种来源: | E. coli |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | T7 |
| 标签/融合蛋白: | His6（插入件上的C端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | Nde 1(销毁后未知) |
| 3' 克隆位点: | Not 1(销毁后未知) |
| 5' 测序引物 | T7 TAATACGACTCACTATAGGG |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PVS10 was a gift from Irina Artsimovitch (Addgene plasmid # 104398 ; http://n2t.net/addgene:104398 ; RRID:Addgene_104398) |
| 参考文献部分: | Purification of bacterial RNA polymerase: tools and protocols. Svetlov V, Artsimovitch I. Methods Mol Biol. 2015;1276:13-29. doi: 10.1007/978-1-4939-2392-2_2. 10.1007/978-1-4939-2392-2_2 PubMed 25665556 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PVS10 15200 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION polycistronic vector for expression of E. coli core RNAP.
ACCESSION .
VERSION .
KEYWORDS PVS10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15200)
AUTHORS Svetlov V, Artsimovitch I
TITLE Purification of bacterial RNA polymerase: tools and protocols.
JOURNAL Methods Mol Biol. 2015;1276:13-29. doi: 10.1007/978-1-4939-2392-2_2.
PUBMED 25665556
REFERENCE 2 (bases 1 to 15200)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..15200
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 12..467
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(99..118)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 309..330
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 493..597
/gene="bla"
/label=AmpR promoter
CDS 598..1458
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(816..835)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1629..2217
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2118..2137
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2371..2388
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2403..2542
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2468..2487)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2628..2650
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2644..2835)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3644..4726)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 4688..4707
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(4727..4804)
/gene="lacI"
/label=lacI promoter
primer_bind 5010..5029
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 5113..5132
/label=T7
/note="T7 promoter, forward primer"
promoter 5113..5131
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5132..5156
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5171..5193
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 14616..14633
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 14753..15028
/codon_start=1
/gene="E. coli rpoZ"
/product="DNA-directed RNA polymerase subunit omega"
/label=RNAP omega
/note="promotes RNA polymerase assembly"
/translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
CDS 15044..15061
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(15114..15132)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 15128..15175
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
2521 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
2701 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
2761 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
2821 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
2881 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
2941 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
3001 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
3061 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
3121 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
3181 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
3241 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
3301 tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
3361 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
3421 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
3481 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
3541 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
3601 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
3661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
3721 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
3781 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
3841 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
3901 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
3961 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
4021 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
4081 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
4141 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
4201 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
4261 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
4321 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
4381 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
4441 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
4501 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
4561 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
4621 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
4681 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
4741 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
4801 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
4861 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
4921 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
4981 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
5041 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
5101 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
5161 tagaaataat tttgtttaac tttaagaagg agatatacat atgcagggtt ctgtgacaga
5221 gtttctaaaa ccgcgcctgg ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac
5281 ccttgagcct ttagagcgtg gctttggcca tactctgggt aacgcactgc gccgtattct
5341 gctctcatcg atgccgggtt gcgcggtgac cgaggttgag attgatggtg tactacatga
5401 gtacagcaca aaagaaggcg ttcaggaaga tatcctggaa atcctgctca acctgaaagg
5461 gctggcggtg agagttcagg gcaaagatga agttattctt accttgaata aatctggcat
5521 tggccctgtg actgcagccg atatcaccca cgacggtgat gtcgaaatcg tcaagccgca
5581 gcacgtgatc tgccacctga ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca
5641 gcgcggtcgt ggttatgtgc cggcttctac ccgaattcat tcggaagaag atgagcgccc
5701 aatcggccgt ctgctggtcg acgcatgcta cagccctgtg gagcgtattg cctacaatgt
5761 tgaagcagcg cgtgtagaac agcgtaccga cctggacaag ctggtcatcg aaatggaaac
5821 caacggcaca atcgatcctg aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca
5881 actggaggct ttcgttgact tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc
5941 agagttcgat ccgatcctgc tgcgccctgt tgacgatctg gaattgactg tccgctctgc
6001 taactgcctt aaagcagaag ctatccacta tatcggtgat ctggtacagc gtaccgaggt
6061 tgagctcctt aaaacgccta accttggtaa aaaatctctt actgagatta aagacgtgct
6121 ggcttcccgt ggactgtctc tgggcatgcg cctggaaaac tggccaccgg caagcatcgc
6181 tgacgagtaa ccggatcccc gatccgtgga cttgtcagcg actgaggaaa cagaccatgg
6241 atccccgatc cgtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga
6301 gaaaaaacgt attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct
6361 cctttctatc cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta
6421 tggtctggaa gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga
6481 gctgcaatac gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat
6541 ccgtggcgtg acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg
6601 cgaagcgccg gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat
6661 tccgctcatg acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc
6721 ccagctgcac cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc
6781 gggtaaagtg ctgtataacg cgcgtatcat cccttaccgt ggttcctggc tggacttcga
6841 attcgatccg aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac
6901 catcattctg cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa
6961 agttatcttt gaaatccgtg ataacaagct gcagatggaa ctggtgccgg aacgcctgcg
7021 tggtgaaacc gcatcttttg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg
7081 ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt
7141 cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg
7201 cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta agctgagcca
7261 gtctggtcac aagcgtatcg aaacgctgtt caccaacgat ctggatcacg gcccatatat
7321 ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta
7381 ccgcatgatg cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa
7441 cctgttcttc tccgaagacc gttatgactt gtctgcggtt ggtcgtatga agttcaaccg
7501 ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg acatcattga
7561 tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca
7621 cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt tccgcgttgg
7681 cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct
7741 gatgccacag gatatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg
7801 ttccagccag ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa
7861 acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag gcttcgaagt
7921 tcgagacgta cacccgactc actacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc
7981 gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat acggcttcct
8041 tgagactccg tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc actacctgtc
8101 tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacttgg atgaagaagg
8161 ccacttcgta gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg
8221 cgaccaggtt gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct
8281 gatcccgttc ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg
8341 tcaggccgtt ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc
8401 tgttgccgtt gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt
8461 ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg
8521 tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca
8581 gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc
8641 gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg
8701 gaatggttac aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg
8761 tttcaccacc atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctgggtcc
8821 ggaagagatc accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga
8881 atccggtatc gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt
8941 aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg
9001 tgagaaagcc tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac
9061 ggttatcgac gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga
9121 aatcgaagaa atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct
9181 cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga
9241 gaagctcgac aaactgccgc gcgatcgctg gctggagctg ggcctgacag acgaagagaa
9301 acaaaatcag ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa
9361 gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct
9421 gaagattgtt aaggtatatc tggcggttaa acgccgtatc cagcctggtg acaagatggc
9481 aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta
9541 cgatgaaaac ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat
9601 gaacatcggt cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa
9661 gatcaacgcc atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg
9721 tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga
9781 agaagttatg cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt
9841 cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc
9901 cggtcagatc cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt
9961 tggttacatg tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc
10021 caccggttct tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg
10081 tcagcgtttc ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct
10141 gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa
10201 catcgtggac ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt
10261 gaaagagatt cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa
10321 acaggtcact gctgtcgggt taaaacccgg cagcggattg tgctaactcc gacgggagca
10381 aatccgtgaa agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg
10441 cgatcaaaat tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta
10501 aaaagccgga aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg
10561 cccgtatctt tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga
10621 aacaccgtgg cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc
10681 gtgagcgtat gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat
10741 cgctgccgtc ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac
10801 tgtactttga atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga
10861 tcctgactga agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga
10921 agatgggggc ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg
10981 aacagctgcg tgaagagctg aacgaaacca actccgaaac caagcgtaaa aagctgacca
11041 agcgtatcaa actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc
11101 tgaccgttct gccggtactg ccgccagatc tgcgtccgct ggttccgctg gatggtggtc
11161 gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc
11221 tgaaacgtct gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc
11281 tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt
11341 ctaacaagcg tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc
11401 gtcagaacct gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc
11461 catacctgcg tctgcatcag tgcggtctgc cgaagaaaat ggcactggag ctgttcaaac
11521 cgttcatcta cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga
11581 aaatggttga gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac
11641 acccggtact gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac
11701 cggtactgat cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg
11761 ccgacttcga tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg
11821 aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa
11881 tcatcgttcc gtctcaggac gttgtactgg gtctgtacta catgacccgt gactgtgtta
11941 acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct
12001 ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg
12061 ctaacggtga attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc
12121 tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa
12181 aagcaatctc caaaatgctg aacacctgct accgcattct cggtctgaaa ccgaccgtta
12241 tttttgcgga ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg
12301 ttggtatcga tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag
12361 cagaagttgc tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct
12421 acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg
12481 ataacctgca aactgaaacc gtgattaacc gtgacggtca ggaagagaag caggtttcct
12541 tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc
12601 agcttgctgg tatgcgtggt ctgatggcga agccggatgg ctccatcatc gaaacgccaa
12661 tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg
12721 ctcgtaaagg tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc
12781 gtctggttga cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag
12841 gtatcatgat gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg
12901 tactgggtcg tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc
12961 cgcgcaacac gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg
13021 cggttaaagt acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct
13081 acggtcgtga cctggcgcgt ggccacatca tcaacaaggg tgaagcaatc ggtgttatcg
13141 cggcacagtc catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg
13201 gtgcggcatc tcgtgcggct gctgaatcca gcatccaagt gaaaaacaaa ggtagcatca
13261 agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta
13321 atactgaact gaaactgatc gacgaattcg gtcgtactaa agaaagctac aaagtacctt
13381 acggtgcggt actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa
13441 actgggaccc gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg
13501 acatgatcga cggccagacc attacgcgtc agaccgacga actgaccggt ctgtcttcgc
13561 tggtggttct ggattccgca gaacgtaccg caggtggtaa agatctgcgt ccggcactga
13621 aaatcgttga tgctcagggt aacgacgttc tgatcccagg taccgatatg ccagcgcagt
13681 acttcctgcc gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg
13741 acaccctggc gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc
13801 cgcgcgttgc ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc ctggctgaaa
13861 tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc
13921 cggtagacgg tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt
13981 tcgaaggtga acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg
14041 acattctgcg tctgcgtggt gttcatgctg ttactcgtta catcgttaac gaagtacagg
14101 acgtataccg tctgcagggc gttaagatta acgataaaca catcgaagtt atcgttcgtc
14161 agatgctgcg taaagctacc atcgttaacg cgggtagctc cgacttcctg gaaggcgaac
14221 aggttgaata ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg
14281 gtgcaactta ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct
14341 tcatctccgc ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg
14401 gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg
14461 caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc
14521 cggctgcacc gcaggtgact gcagaagacg catctgccag cctggcagaa ctgctgaacg
14581 caggtctggg cggttctgat aacgagctcg aggtgcacca tcaccatcac cattaaggcc
14641 tcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag
14701 catttgtcag aataagcttc aatgactgca gacttaagaa ggagattaat gtatggcacg
14761 cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg tactggtcgc
14821 cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac cggaagaaaa
14881 cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca acaaccagat
14941 cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat tacaagccgt
15001 taccgctatt gctgaaggtc gtcgttaagc ggccgcactc gagcaccacc accaccacca
15061 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
15121 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
15181 aggaggaact atatccggat
//
