Flag-HA-USP36

LOCUS        Flag-HA-USP36        9812 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP36
FEATURES             Location/Qualifiers
     source          1..9812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2026..2044
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2082..2104)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2204..2223
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2417..2439
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2567..2586
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2786..3302
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3366..3707
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3774..4190
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4120..4142
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4158..4174
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      4203..4212
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4212..4235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4248..4274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4290..4314
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7699..7723)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7736..8303
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7753..8305
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7897..7914)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8124..8143
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8317..8916
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8317..8336)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8813..8833
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8992..9506
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    9668..9684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9692..9698,9699..9716,9717..9722))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9737..9758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2041 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2101 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2161 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2281 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2341 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2401 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     2461 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     2521 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     2581 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     2641 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     2701 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     2761 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2821 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     2881 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     2941 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     3001 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     3061 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     3121 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     3181 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
     3241 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
     3301 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
     3361 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
     3421 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     3481 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     3541 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     3601 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     3661 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     3721 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
     3781 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     3841 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     3901 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     3961 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     4021 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     4081 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     4141 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
     4201 ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg
     4261 ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcgcgccca
     4321 ccatgccaat agtggataag ttgaaggagg ccctgaaacc cggccgcaag gactcggctg
     4381 atgatggaga actggggaag cttcttgcct cctctgccaa gaaggtcctt ttacagaaaa
     4441 tcgagttcga gccagccagc aagagcttct cctaccagct ggaggcctta aagagcaaat
     4501 atgtgttgct caaccccaaa acagagggag ctagtcgcca caagagtgga gatgacccac
     4561 cggccgggag acagggcagt gagcacacgt atgagagctg tggtgacgga gtcccagccc
     4621 cgcagaaagt gcttttcccc acggagcgac tgtctctgag gtgggagcgg gtcttccgcg
     4681 tgggcgcagg actccacaac cttggcaaca cctgctttct caatgccacc atccagtgct
     4741 tgacctacac accacctcta gccaactacc tgctctccaa ggagcatgct cgcagctgcc
     4801 accagggaag cttctgcatg ctgtgtgtca tgcagaacca cattgtccag gccttcgcca
     4861 acagcggcaa cgccatcaag cccgtctcct tcatccgaga cctgaaaaag atcgcccgac
     4921 acttccgctt tgggaaccag gaggacgcgc atgagttcct gcggtacacc atcgacgcca
     4981 tgcagaaagc ctgcctgaat ggctgtgcca agttggatcg tcaaacgcag gctactacct
     5041 tggtccatca aatttttgga gggtatctca gatcacgcgt gaagtgctcc gtgtgcaaga
     5101 gcgtctcgga cacctacgac ccctacttgg acgtcgcgct ggagatccgg caagctgcga
     5161 atattgtgcg tgctctggaa ctttttgtga aagcagatgt cctgagtgga gagaatgcct
     5221 acatgtgtgc taaatgcaag aagaaggttc cagccagcaa gcgcttcacc atccacagaa
     5281 catccaacgt cttaaccctt tccctcaagc gctttgccaa cttcagcggg gggaagatca
     5341 ccaaggatgt aggctatccg gaattcctca acatacgtcc gtatatgtcc cagaataatg
     5401 gtgatcctgt catgtatgga ctctatgctg tcctggtgca ctcgggctac agctgccatg
     5461 ccgggcacta ttactgctac gtgaaggcaa gcaatggaca gtggtaccag atgaatgatt
     5521 ccttggtcca ttccagcaac gtcaaggtgg ttctgaacca gcaggcctac gtgctgttct
     5581 atctgcgaat tccaggctct aagaaaagtc ccgagggcct catctccagg acaggctcct
     5641 cctcccttcc cggccgcccg agtgtgattc cagatcactc caagaagaac atcggcaatg
     5701 ggattatttc ctccccactg actggaaagc gacaagactc tgggacgatg aagaagccgc
     5761 acaccactga agagattggt gtgcccatat ccaggaatgg ctccaccctg ggcctgaagt
     5821 cccagaacgg ctgcattcct ccaaagctgc cctcggggtc cccttccccc aaactctccc
     5881 agacacccac acacatgcca accatcctag acgaccctgg aaagaaggtg aagaagccag
     5941 ctcctccaca gcacttttcc cccagaactg ctcaggggct gcctgggacc agcaactcga
     6001 atagcagcag atctgggagc caaaggcagg gctcctggga cagcagggat gttgtcctct
     6061 ctacctcacc taagctcctg gctacagcca ctgccaacgg gcatgggctg aaggggaacg
     6121 acgagagcgc tggcctcgac aggaggggct ccagcagctc cagcccagag cactcggcca
     6181 gcagtgactc caccaaggcc ccccagaccc ccaggagtgg agcggcccat ctctgcgatt
     6241 ctcaggaaac gaactgttcc accgctggcc actccaaaac gccgccaagt ggagcagatt
     6301 ctaagacggt gaagctgaag tcccctgtcc tgagcaacac caccactgag cctgcaagca
     6361 ccatgtctcc tccaccagcc aaaaaactgg ccctttctgc caagaaggcc agcaccctgt
     6421 ggagggcgac cggcaatgac ctccgtccac ctcccccctc accatcctcc gacctcaccc
     6481 accccatgaa aacctctcac cccgtcgttg cctccacttg gcccgtccat agagccaggg
     6541 ctgtgtcacc tgctccccaa tcatccagcc gcctgcaacc ccccttcagc ccccacccca
     6601 cattgctgtc cagtaccccc aagcccccag ggacgtcaga accacggagc tgctcctcca
     6661 tctcgacggc gctgcctcag gtcaacgagg accttgtgtc tcttccacac cagttgccag
     6721 aggccagtga gcccccccag agcccctctg agaagaggaa aaagaccttt gtgggagagc
     6781 cgcagaggct gggctcagag acgcgcctcc cacagcacat cagggaggcc actgcggctc
     6841 cccacgggaa gaggaagagg aagaagaaga agcgcccgga ggacacagct gccagcgccc
     6901 tgcaggaggg gcagacacag agacagcctg ggagccccat gtacaggagg gagggccagg
     6961 cacagctgcc cgctgtcaga cggcaggaag atggcacaca gccacaggtg aatggccagc
     7021 aggtgggatg tgttacggac ggccaccacg cgagcagcag gaagcggagg aggaaaggag
     7081 cagaaggtct tggtgaagaa ggcggcctgc accaggaccc acttcggcac agctgctctc
     7141 ccatgggtga tggtgatcca gaggccatgg aagagtctcc aaggaaaaag aaaaagaaaa
     7201 aaagaaagca ggagacacag cgggcagtag aagaggatgg gcatctcaaa tgcccaagga
     7261 gtgccaagcc ccaagatgct gttgtccccg agtccagcag ctgcgcacca tccgcgaatg
     7321 gctggtgtcc tggggaccgc atggggctga gccaggcccc tcctgtgtct tggaatggag
     7381 agcgggagtc tgatgtggtc caggaactgc tcaaatactc atctgataaa gcttacggga
     7441 gaaaagttct gacctgggat ggcaagatgt cggcggtcag tcaggatgct attgaagaca
     7501 gcagacaggc ccggactgag accgtggttg atgactggga cgaagagttt gaccgaggga
     7561 aggaaaagaa aattaaaaaa tttaagagag agaagaggag aaacttcaac gccttccaga
     7621 aacttcagac tcgacggaac ttctggtctg tgactcaccc agcaaaggct gccagcctca
     7681 gctatcgccg cttaattaat ccaactttct tgtacaaagt ggttcgataa cgaattccgc
     7741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     7801 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     7861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     7921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     7981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     8041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     8101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     8161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     8221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     8281 tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa gcccacggtg
     8341 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     8401 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     8461 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     8521 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     8581 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     8641 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     8701 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     8761 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     8821 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     8881 cgcacctggt gcatgacccg caagcccggt gcctgatgca tcgatgtcga cctgcagcca
     8941 agcttatcga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     9001 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata
     9061 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
     9121 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     9181 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     9241 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     9301 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     9361 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct
     9421 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     9481 actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt
     9541 ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca
     9601 catactccaa tactcctgaa atagttcatt atggacagcg cagaaagagc tggggagaat
     9661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9781 ctttccagtc gggaaacctg tcgtgccagc tg
//