| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-EF1α-DsRed-Monomer-C1, pLVX-EF1a-DsRed-Monomer-C1, pLVX-EF1alpha-DsRed-Monomer-C1 |
| 质粒类型: | 慢病毒表达载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | EF1α |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 9485 bp |
| 5' 测序引物及序列: | EF1a Forward: TCAAGCCTCAGACAGTGGTTC |
| 3' 测序引物及序列: | -- |
| 载体标签: | N-DsRed-Monomer |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素(Puromycin) |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系，293、CV-1、CHO等 |
| 备注: |
pLVX-EF1α-DsRed-Monomer-C1载体能够表达N端DsRed Monomer融合蛋白； DsRed-Monomer是DsRed的单体突变体，与DsRed相比，编码序列经过了人工优化，适用于在哺乳动物细胞中的高水平表达； EF1α启动子是高表达启动子，对于那些使用CMV启动子表达效果不理想的细胞系具有良好效果。 |
| 产品目录号: | 631989 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1a-DsRed-Monomer-C1 9485 bp DNA circular 22-MAR-2010
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 631989
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=Packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
misc_feature 5453..6044
/label=WPRE
rep_origin complement(7353..8026)
/label=pUC\origin
CDS complement(8171..9167)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 6247..6883
/label=LTR
misc_feature 3547..4221
/label=DsRed\Monomer
misc_feature 4296..4298
/label=Stop
misc_feature 4300..4302
/label=Stop
misc_feature 4304..4306
/label=Stop
misc_feature 4235..4288
/label=MCS
misc_feature 4311..4819
/label=PGK\Promoter
misc_feature 4840..5439
/label=Puro(R)
promoter 2185..3519
/label=EF1a\Promoter
BASE COUNT 2246 a 2484 c 2600 g 2155 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggtc
3541 gccaccatgg acaacaccga ggacgtcatc aaggagttca tgcagttcaa ggtgcgcatg
3601 gagggctccg tgaacggcca ctacttcgag atcgagggcg agggcgaggg caagccctac
3661 gagggcaccc agaccgccaa gctgcaggtg accaagggcg gccccctgcc cttcgcctgg
3721 gacatcctgt ccccccagtt ccagtacggc tccaaggcct acgtgaagca ccccgccgac
3781 atccccgact acatgaagct gtccttcccc gagggcttca cctgggagcg ctccatgaac
3841 ttcgaggacg gcggcgtggt ggaggtgcag caggactcct ccctgcagga cggcaccttc
3901 atctacaagg tgaagttcaa gggcgtgaac ttccccgccg acggccccgt aatgcagaag
3961 aagactgccg gctgggagcc ctccaccgag aagctgtacc cccaggacgg cgtgctgaag
4021 ggcgagatct cccacgccct gaagctgaag gacggcggcc actacacctg cgacttcaag
4081 accgtgtaca aggccaagaa gcccgtgcag ctgcccggca accactacgt ggactccaag
4141 ctggacatca ccaaccacaa cgaggactac accgtggtgg agcagtacga gcacgccgag
4201 gcccgccact ccggctccca gtccggactc agatctcgag ctcaagcttc gaattctgca
4261 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aattctaccg
4321 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
4381 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
4441 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
4501 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
4561 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
4621 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
4681 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
4741 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
4801 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
4861 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
4921 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
4981 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
5041 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
5101 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
5161 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
5221 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
5281 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
5341 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
5401 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc
5461 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
5521 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
5581 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
5641 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
5701 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
5761 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
5821 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
5881 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
5941 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
6001 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
6061 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
6121 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
6181 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
6241 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
6301 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
6361 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
6421 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
6481 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
6541 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
6601 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
6661 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
6721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
6781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
6841 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
6901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
6961 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
7021 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
7081 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
7141 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
7201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
7261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
7321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
7381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
7441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
7501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
7561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
7621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
7681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
7741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
7801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
7861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
7921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
7981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
8041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
8101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
8161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
8221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
8281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
8341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
8401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
8461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
8521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
8581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
8641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
8701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
8761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
8821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
8881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
8941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
9001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
9061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
9121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
9181 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
9241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
9301 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
9361 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
9421 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
9481 gtagt
//
