pLVX-EF1α-DsRed-Monomer-C1

价格:1350元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-EF1α-DsRed-Monomer-C1, pLVX-EF1a-DsRed-Monomer-C1, pLVX-EF1alpha-DsRed-Monomer-C1
质粒类型: 慢病毒表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
启动子: EF1α
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9485 bp
5' 测序引物及序列: EF1a Forward: TCAAGCCTCAGACAGTGGTTC
3' 测序引物及序列: --
载体标签: N-DsRed-Monomer
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系,293、CV-1、CHO等
备注: pLVX-EF1α-DsRed-Monomer-C1载体能够表达N端DsRed Monomer融合蛋白;
DsRed-Monomer是DsRed的单体突变体,与DsRed相比,编码序列经过了人工优化,适用于在哺乳动物细胞中的高水平表达;
EF1α启动子是高表达启动子,对于那些使用CMV启动子表达效果不理想的细胞系具有良好效果。
产品目录号: 631989
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-EF1α-DsRed-Monomer-C1载体图谱



pLVX-EF1α-DsRed-Monomer-C1多克隆位点

pLVX-EF1α-DsRed-Monomer-C1载体特征

载体简介

载体序列

LOCUS       pLVX-EF1a-DsRed-Monomer-C1   9485 bp    DNA   circular            22-MAR-2010
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     Cat. No. 631989
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     misc_feature    5453..6044
                     /label=WPRE
     rep_origin      complement(7353..8026)
                     /label=pUC\origin
     CDS             complement(8171..9167)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             6247..6883
                     /label=LTR
     misc_feature    3547..4221
                     /label=DsRed\Monomer
     misc_feature    4296..4298
                     /label=Stop
     misc_feature    4300..4302
                     /label=Stop
     misc_feature    4304..4306
                     /label=Stop
     misc_feature    4235..4288
                     /label=MCS
     misc_feature    4311..4819
                     /label=PGK\Promoter
     misc_feature    4840..5439
                     /label=Puro(R)
     promoter        2185..3519
                     /label=EF1a\Promoter
BASE COUNT     2246 a      2484 c      2600 g      2155 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggtc 
     3541 gccaccatgg acaacaccga ggacgtcatc aaggagttca tgcagttcaa ggtgcgcatg 
     3601 gagggctccg tgaacggcca ctacttcgag atcgagggcg agggcgaggg caagccctac 
     3661 gagggcaccc agaccgccaa gctgcaggtg accaagggcg gccccctgcc cttcgcctgg 
     3721 gacatcctgt ccccccagtt ccagtacggc tccaaggcct acgtgaagca ccccgccgac 
     3781 atccccgact acatgaagct gtccttcccc gagggcttca cctgggagcg ctccatgaac 
     3841 ttcgaggacg gcggcgtggt ggaggtgcag caggactcct ccctgcagga cggcaccttc 
     3901 atctacaagg tgaagttcaa gggcgtgaac ttccccgccg acggccccgt aatgcagaag 
     3961 aagactgccg gctgggagcc ctccaccgag aagctgtacc cccaggacgg cgtgctgaag 
     4021 ggcgagatct cccacgccct gaagctgaag gacggcggcc actacacctg cgacttcaag 
     4081 accgtgtaca aggccaagaa gcccgtgcag ctgcccggca accactacgt ggactccaag 
     4141 ctggacatca ccaaccacaa cgaggactac accgtggtgg agcagtacga gcacgccgag 
     4201 gcccgccact ccggctccca gtccggactc agatctcgag ctcaagcttc gaattctgca 
     4261 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aattctaccg 
     4321 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 
     4381 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 
     4441 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 
     4501 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 
     4561 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 
     4621 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 
     4681 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 
     4741 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 
     4801 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 
     4861 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 
     4921 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 
     4981 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 
     5041 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 
     5101 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 
     5161 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 
     5221 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 
     5281 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 
     5341 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 
     5401 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 
     5461 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
     5521 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
     5581 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
     5641 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
     5701 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
     5761 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
     5821 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 
     5881 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
     5941 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
     6001 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 
     6061 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 
     6121 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 
     6181 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 
     6241 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 
     6301 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 
     6361 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 
     6421 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 
     6481 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 
     6541 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 
     6601 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 
     6661 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 
     6721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 
     6781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 
     6841 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 
     6901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 
     6961 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
     7021 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     7081 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     7141 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     7201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     7261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     7321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     7381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     7441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     7501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     7561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     7621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     7681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     7741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     7801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     7861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     7921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     7981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     8041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     8101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     8161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     8221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     8281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     8341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     8401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     8461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     8521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     8581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     8641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     8701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     8761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     8821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     8881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     8941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     9001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     9061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     9121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 
     9181 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     9241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     9301 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 
     9361 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 
     9421 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 
     9481 gtagt 
//

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