pLP1

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pLP1
质粒类型: 哺乳动物细胞慢病毒包装载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8889 bp
5' 测序引物及序列: CMV-F:  CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
备注:
载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组几率。pLP/VSVG, pLP1, pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。

第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG(或者pCMV-VSVG)。在使用的时候,按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时,对于60mm的细胞培养皿来说,按照慢病毒基因质粒(3ug)和package mix(6 ug,也就是6 ul)的比例进行慢病毒质粒包装实验。
 
产品目录号: K4970-00
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLP1载体图谱


pLP1载体特征

载体简介

载体序列

LOCUS       pLP1	8889 bp 	DNA     circular	SYN
DEFINITION  pLP1
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="pLP1"
                     /mol_type="other DNA"
     promoter        80..656
                     /label="CMV_immearly_promoter"
     misc_feature    159..446
                     /label="CAG_enhancer"
     misc_feature    613..633
                     /label="CMV_fwd_primer"
     promoter        614..683
                     /label="CMV_promoter"
     promoter        626..745
                     /label="CMV2_promoter"
     misc_feature    657..676
                     /label="pCEP_fwd_primer"
     misc_feature    659..683
                     /label="LNCX_primer"
     misc_feature    933..1316
                     /label="bGlob_int"
     gene            1355..2857
                     /label="Gag_HIV (variant)"
                     /gene="Gag_HIV (variant)"
     CDS             1355..2857
                     /label="ORF frame 2"
     CDS             2923..5661
                     /label="ORF frame 1"
     misc_feature    5350..5365
                     /label="cPPT"
     CDS             5606..6124
                     /label="ORF frame 2"
     misc_feature    5686..5919
                     /label="RRE"
     gene            complement(7813..8673)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7813..8673)
                     /label="ORF frame 1"
     promoter        complement(8715..8743)
                     /label="AmpR_promoter"
     misc_feature    complement(8847..8866)
                     /label="SV40pro_F_primer"
ORIGIN
    1 TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC
   61 CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
  121 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
  181 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
  241 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
  301 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
  361 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
  421 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA
  481 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG
  541 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT
  601 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
  661 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
  721 GAAGACACCG GGACCGATCC AGCCTCCCCT CGAAGCTTAC ATGTGGTACC GAGCTCGGAT
  781 CCTGAGAACT TCAGGGTGAG TCTATGGGAC CCTTGATGTT TTCTTTCCCC TTCTTTTCTA
  841 TGGTTAAGTT CATGTCATAG GAAGGGGAGA AGTAACAGGG TACACATATT GACCAAATCA
  901 GGGTAATTTT GCATTTGTAA TTTTAAAAAA TGCTTTCTTC TTTTAATATA CTTTTTTGTT
  961 TATCTTATTT CTAATACTTT CCCTAATCTC TTTCTTTCAG GGCAATAATG ATACAATGTA
 1021 TCATGCCTCT TTGCACCATT CTAAAGAATA ACAGTGATAA TTTCTGGGTT AAGGCAATAG
 1081 CAATATTTCT GCATATAAAT ATTTCTGCAT ATAAATTGTA ACTGATGTAA GAGGTTTCAT
 1141 ATTGCTAATA GCAGCTACAA TCCAGCTACC ATTCTGCTTT TATTTTATGG TTGGGATAAG
 1201 GCTGGATTAT TCTGAGTCCA AGCTAGGCCC TTTTGCTAAT CATGTTCATA CCTCTTATCT
 1261 TCCTCCCACA GCTCCTGGGC AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG
 1321 CACGTGAGAT CTGAATTCGA GATCTGCCGC CGCCATGGGT GCGAGAGCGT CAGTATTAAG
 1381 CGGGGGAGAA TTAGATCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA
 1441 TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG
 1501 CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA
 1561 GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA
 1621 TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA
 1681 CAAAAGTAAG AAAAAAGCAC AGCAAGCAGC AGCTGACACA GGACACAGCA ATCAGGTCAG
 1741 CCAAAATTAC CCTATAGTGC AGAACATCCA GGGGCAAATG GTACATCAGG CCATATCACC
 1801 TAGAACTTTA AATGCATGGG TAAAAGTAGT AGAAGAGAAG GCTTTCAGCC CAGAAGTGAT
 1861 ACCCATGTTT TCAGCATTAT CAGAAGGAGC CACCCCACAA GATTTAAACA CCATGCTAAA
 1921 CACAGTGGGG GGACATCAAG CAGCCATGCA AATGTTAAAA GAGACCATCA ATGAGGAAGC
 1981 TGCAGAATGG GATAGAGTGC ATCCAGTGCA TGCAGGGCCT ATTGCACCAG GCCAGATGAG
 2041 AGAACCAAGG GGAAGTGACA TAGCAGGAAC TACTAGTACC CTTCAGGAAC AAATAGGATG
 2101 GATGACACAT AATCCACCTA TCCCAGTAGG AGAAATCTAT AAAAGATGGA TAATCCTGGG
 2161 ATTAAATAAA ATAGTAAGAA TGTATAGCCC TACCAGCATT CTGGACATAA GACAAGGACC
 2221 AAAGGAACCC TTTAGAGACT ATGTAGACCG ATTCTATAAA ACTCTAAGAG CCGAGCAAGC
 2281 TTCACAAGAG GTAAAAAATT GGATGACAGA AACCTTGTTG GTCCAAAATG CGAACCCAGA
 2341 TTGTAAGACT ATTTTAAAAG CATTGGGACC AGGAGCGACA CTAGAAGAAA TGATGACAGC
 2401 ATGTCAGGGA GTGGGGGGAC CCGGCCATAA AGCAAGAGTT TTGGCTGAAG CAATGAGCCA
 2461 AGTAACAAAT CCAGCTACCA TAATGATACA GAAAGGCAAT TTTAGGAACC AAAGAAAGAC
 2521 TGTTAAGTGT TTCAATTGTG GCAAAGAAGG GCACATAGCC AAAAATTGCA GGGCCCCTAG
 2581 GAAAAAGGGC TGTTGGAAAT GTGGAAAGGA AGGACACCAA ATGAAAGATT GTACTGAGAG
 2641 ACAGGCTAAT TTTTTAGGGA AGATCTGGCC TTCCCACAAG GGAAGGCCAG GGAATTTTCT
 2701 TCAGAGCAGA CCAGAGCCAA CAGCCCCACC AGAAGAGAGC TTCAGGTTTG GGGAAGAGAC
 2761 AACAACTCCC TCTCAGAAGC AGGAGCCGAT AGACAAGGAA CTGTATCCTT TAGCTTCCCT
 2821 CAGATCACTC TTTGGCAGCG ACCCCTCGTC ACAATAAAGA TAGGGGGGCA ATTAAAGGAA
 2881 GCTCTATTAG ATACAGGAGC AGATGATACA GTATTAGAAG AAATGAATTT GCCAGGAAGA
 2941 TGGAAACCAA AAATGATAGG GGGAATTGGA GGTTTTATCA AAGTAAGACA GTATGATCAG
 3001 ATACTCATAG AAATCTGCGG ACATAAAGCT ATAGGTACAG TATTAGTAGG ACCTACACCT
 3061 GTCAACATAA TTGGAAGAAA TCTGTTGACT CAGATTGGCT GCACTTTAAA TTTTCCCATT
 3121 AGTCCTATTG AGACTGTACC AGTAAAATTA AAGCCAGGAA TGGATGGCCC AAAAGTTAAA
 3181 CAATGGCCAT TGACAGAAGA AAAAATAAAA GCATTAGTAG AAATTTGTAC AGAAATGGAA
 3241 AAGGAAGGAA AAATTTCAAA AATTGGGCCT GAAAATCCAT ACAATACTCC AGTATTTGCC
 3301 ATAAAGAAAA AAGACAGTAC TAAATGGAGA AAATTAGTAG ATTTCAGAGA ACTTAATAAG
 3361 AGAACTCAAG ATTTCTGGGA AGTTCAATTA GGAATACCAC ATCCTGCAGG GTTAAAACAG
 3421 AAAAAATCAG TAACAGTACT GGATGTGGGC GATGCATATT TTTCAGTTCC CTTAGATAAA
 3481 GACTTCAGGA AGTATACTGC ATTTACCATA CCTAGTATAA ACAATGAGAC ACCAGGGATT
 3541 AGATATCAGT ACAATGTGCT TCCACAGGGA TGGAAAGGAT CACCAGCAAT ATTCCAGTGT
 3601 AGCATGACAA AAATCTTAGA GCCTTTTAGA AAACAAAATC CAGACATAGT CATCTATCAA
 3661 TACATGGATG ATTTGTATGT AGGATCTGAC TTAGAAATAG GGCAGCATAG AACAAAAATA
 3721 GAGGAACTGA GACAACATCT GTTGAGGTGG GGATTTACCA CACCAGACAA AAAACATCAG
 3781 AAAGAACCTC CATTCCTTTG GATGGGTTAT GAACTCCATC CTGATAAATG GACAGTACAG
 3841 CCTATAGTGC TGCCAGAAAA GGACAGCTGG ACTGTCAATG ACATACAGAA ATTAGTGGGA
 3901 AAATTGAATT GGGCAAGTCA GATTTATGCA GGGATTAAAG TAAGGCAATT ATGTAAACTT
 3961 CTTAGGGGAA CCAAAGCACT AACAGAAGTA GTACCACTAA CAGAAGAAGC AGAGCTAGAA
 4021 CTGGCAGAAA ACAGGGAGAT TCTAAAAGAA CCGGTACATG GAGTGTATTA TGACCCATCA
 4081 AAAGACTTAA TAGCAGAAAT ACAGAAGCAG GGGCAAGGCC AATGGACATA TCAAATTTAT
 4141 CAAGAGCCAT TTAAAAATCT GAAAACAGGA AAGTATGCAA GAATGAAGGG TGCCCACACT
 4201 AATGATGTGA AACAATTAAC AGAGGCAGTA CAAAAAATAG CCACAGAAAG CATAGTAATA
 4261 TGGGGAAAGA CTCCTAAATT TAAATTACCC ATACAAAAGG AAACATGGGA AGCATGGTGG
 4321 ACAGAGTATT GGCAAGCCAC CTGGATTCCT GAGTGGGAGT TTGTCAATAC CCCTCCCTTA
 4381 GTGAAGTTAT GGTACCAGTT AGAGAAAGAA CCCATAATAG GAGCAGAAAC TTTCTATGTA
 4441 GATGGGGCAG CCAATAGGGA AACTAAATTA GGAAAAGCAG GATATGTAAC TGACAGAGGA
 4501 AGACAAAAAG TTGTCCCCCT AACGGACACA ACAAATCAGA AGACTGAGTT ACAAGCAATT
 4561 CATCTAGCTT TGCAGGATTC GGGATTAGAA GTAAACATAG TGACAGACTC ACAATATGCA
 4621 TTGGGAATCA TTCAAGCACA ACCAGATAAG AGTGAATCAG AGTTAGTCAG TCAAATAATA
 4681 GAGCAGTTAA TAAAAAAGGA AAAAGTCTAC CTGGCATGGG TACCAGCACA CAAAGGAATT
 4741 GGAGGAAATG AACAAGTAGA TAAATTGGTC AGTGCTGGAA TCAGGAAAGT ACTATTTTTA
 4801 GATGGAATAG ATAAGGCCCA AGAAGAACAT GAGAAATATC ACAGTAATTG GAGAGCAATG
 4861 GCTAGTGATT TTAACCTACC ACCTGTAGTA GCAAAAGAAA TAGTAGCCAG CTGTGATAAA
 4921 TGTCAGCTAA AAGGGGAAGC CATGCATGGA CAAGTAGACT GTAGCCCAGG AATATGGCAG
 4981 CTAGATTGTA CACATTTAGA AGGAAAAGTT ATCTTGGTAG CAGTTCATGT AGCCAGTGGA
 5041 TATATAGAAG CAGAAGTAAT TCCAGCAGAG ACAGGGCAAG AAACAGCATA CTTCCTCTTA
 5101 AAATTAGCAG GAAGATGGCC AGTAAAAACA GTACATACAG ACAATGGCAG CAATTTCACC
 5161 AGTACTACAG TTAAGGCCGC CTGTTGGTGG GCGGGGATCA AGCAGGAATT TGGCATTCCC
 5221 TACAATCCCC AAAGTCAAGG AGTAATAGAA TCTATGAATA AAGAATTAAA GAAAATTATA
 5281 GGACAGGTAA GAGATCAGGC TGAACATCTT AAGACAGCAG TACAAATGGC AGTATTCATC
 5341 CACAATTTTA AAAGAAAAGG GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC
 5401 ATAATAGCAA CAGACATACA AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT
 5461 TTTCGGGTTT ATTACAGGGA CAGCAGAGAT CCAGTTTGGA AAGGACCAGC AAAGCTCCTC
 5521 TGGAAAGGTG AAGGGGCAGT AGTAATACAA GATAATAGTG ACATAAAAGT AGTGCCAAGA
 5581 AGAAAAGCAA AGATCATCAG GGATTATGGA AAACAGATGG CAGGTGATGA TTGTGTGGCA
 5641 AGTAGACAGG ATGAGGATTA ACACATGGAA TTCCGGAGCG GCCGCAGGAG CTTTGTTCCT
 5701 TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA
 5761 GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA
 5821 GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT
 5881 CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG
 5941 AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA
 6001 ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG
 6061 CTTCCGCGGA ATTCACCCCA CCAGTGCAGG CTGCCTATCA GAAAGTGGTG GCTGGTGTGG
 6121 CTAATGCCCT GGCCCACAAG TATCACTAAG CTCGCTTTCT TGCTGTCCAA TTTCTATTAA
 6181 AGGTTCCTTT GTTCCCTAAG TCCAACTACT AAACTGGGGG ATATTATGAA GGGCCTTGAG
 6241 CATCTGGATT CTGCCTAATA AAAAACATTT ATTTTCATTG CAATGATGTA TTTAAATTAT
 6301 TTCTGAATAT TTTACTAAAA AGGGAATGTG GGAGGTCAGT GCATTTAAAA CATAAAGAAA
 6361 TGAAGAGCTA GTTCAAACCT TGGGAAAATA CACTATATCT TAAACTCCAT GAAAGAAGGT
 6421 GAGGCTGCAA ACAGCTAATG CACATTGGCA ACAGCCCCTG ATGCCTATGC CTTATTCATC
 6481 CCTCAGAAAA GGATTCAAGT AGAGGCTTGA TTTGGAGGTT AAAGTTTTGC TATGCTGTAT
 6541 TTTACATTAC TTATTGTTTT AGCTGTCCTC ATGAATGTCT TTTCACTACC CATTTGCTTA
 6601 TCCTGCATCT CTCAGCCTTG ACTCCACTCA GTTCTCTTGC TTAGAGATAC CACCTTTCCC
 6661 CTGAAGTGTT CCTTCCATGT TTTACGGCGA GATGGTTTCT CCTCGCCTGG CCACTCAGCC
 6721 TTAGTTGTCT CTGTTGTCTT ATAGAGGTCT ACTTGAAGAA GGAAAAACAG GGGGCATGGT
 6781 TTGACTGTCC TGTGAGCCCT TCTTCCCTGC CTCCCCCACT CACAGTGACC CGGAATCCCT
 6841 CGACATGGCA GTCTAGCACT AGTGCGGCCG CAGATCTGCT TCCTCGCTCA CTGACTCGCT
 6901 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
 6961 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
 7021 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
 7081 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
 7141 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
 7201 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
 7261 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
 7321 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
 7381 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
 7441 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
 7501 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
 7561 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
 7621 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
 7681 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
 7741 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
 7801 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
 7861 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
 7921 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
 7981 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
 8041 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
 8101 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
 8161 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
 8221 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
 8281 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
 8341 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
 8401 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
 8461 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
 8521 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
 8581 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
 8641 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
 8701 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
 8761 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGGGA TCCCCTGAGG
 8821 GGGCCCCCAT GGGCTAGAGG ATCCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA
 8881 GCCATGAGC
//

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