pRS414

价格：1500元

联系方式：I47-825O-882O

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载体基本信息

出品公司:	Stratagene（Agilent）
载体名称:	pRS414
质粒类型:	酿酒酵母载体；酵母着丝粒质粒；穿梭载体
克隆方法:	--
高拷贝/低拷贝:	多拷贝
启动子:	--
载体大小:	4788 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	Ampicillin
筛选标记:	TRP1
克隆宿主:	--
备注:	着丝粒: CEN6；含a beta-gal报告基因；宿主菌株YPH499，YPH500，YPH501
产品目录号:	--
稳定性:	--
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

载体图谱质粒图谱和多克隆位点信息

pRS414载体图谱

载体简介

载体序列

LOCUS       pRS414	4788 bp 	DNA	SYN
DEFINITION  pRS414
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="pRS414"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     gene            468..1142
                     /label="TRP1"
                     /gene="TRP1"
     CDS             468..1142
                     /label="ORF frame 3"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     CDS             complement(1479..2057)
                     /label="ORF frame 2"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(1712..1855)
                     /label="lacZ_a"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    1826..1848
                     /label="M13_pUC_fwd_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        1841..1857
                     /label="M13_forward20_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        1867..1885
                     /label="T7_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    1910..1926
                     /label="pBluescriptKS_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(1961..1977)
                     /label="pBluescriptSK_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(2013..2032)
                     /label="T3_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(2049..2067)
                     /label="M13_reverse_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(2066..2088)
                     /label="M13_pUC_rev_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            complement(3214..4074)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             complement(3214..4074)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4116..4144)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4206..4724
                     /label="CEN6_ARS4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
  241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
  301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
  361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
  421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
  481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
  541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
  601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
  661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
  721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
  781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
  841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
  901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
  961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
 1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
 1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
 1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
 1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
 1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
 1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
 1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
 1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
 1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
 1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
 1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
 1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
 1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
 1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
 1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
 1921 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg
 1981 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
 2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
 2101 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact
 2161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
 2221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
 2281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
 2341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
 2401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
 2461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
 2521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
 2581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
 2641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
 2701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
 2761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
 2821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
 2881 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
 2941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
 3001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
 3061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
 3121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
 3181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
 3241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
 3301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
 3361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
 3421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
 3481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
 3541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
 3601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
 3661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
 3721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
 3781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
 3841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
 3901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
 3961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
 4021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
 4081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
 4141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
 4201 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
 4261 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
 4321 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
 4381 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
 4441 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
 4501 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
 4561 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
 4621 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
 4681 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta
 4741 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//

pBHR68	pPH3
pFN31K Nluc-CMV-neo-Flexi	pGL101
pYES2-EGFP	pMV261
pG5luc	pFtsH_TA
pZeoSV2 (+)	pGL4.77[hRlucP/Hygro]
pCMV-3Tag-3a	pAc5.1a
pLVX-rHom-Sec1	pIB/V5-His
pXIS11	pKO11
pSW510	pIJ702-87del(78-86, 88-132)
pSilencer5.1-H1 Retro	pcDNA6.2/V5-PL-DEST
pAX01-ComK	pTrcHis2 C
pAc5.1-V5-His A	pDEST10
pME12::Tn5-259	pCambia1201
pCas-Guide-EF1a-GFP	pYES2-kan
pET302/NT-His	pcDNA3.1+/N-GST(TEV)
pGL4.25[luc2CP/minP]	VaD1
pWW60-1	pLenti CMV/TO Neo empty (w215-1)
pCTAP-Shuttle-A	pJW342
pET-12c(+)	pHC79-2cos/tk
pFB-hrGFP	pHIC-PI
pTCK303	pET-32 EK/LIC
pFastBac1	pBApo-CMV-Pur
pcDNA3.1/His B	pTT5
pVW5JI	pB-gyrase_TA
pECFP-Nuc	phCMV1
p53-Luc	pLKO-DEST-EGFP
pET-28a-EGFP	pE
DR2	pcDNA3.1+P2A-eGFP
pRS426	pMKO.1-GFP
pEF4/His B	pDR540
pCas	pTYB2
pacAd5 9.2-100	pMMB33
pLVX-Myc-MCS-Strep-IRES-Puro	pBad/Myc-His A
pCDF1-MCS2-EF1-Puro	pIAβ8
pFastBac-GST-C	pcDNA4/V5-His B
pMYs-IRES-GFP	pDP5rs
pCB1535	pPZP122
pUCDM	pSC101 and pKG2
IR2	pKLAC2
pFastBac Dual	pSRE-Luc
pMIB/V5-His A	PX391
pcDNA4/HisMax A	p3xFLAG-KV2.1
pTALETF_v2 (NG)	pCMV5
pGL4.39[luc2P/ATF6 RE/Hygro]	pGL4.83[hRlucP/Puro]
pOS1	pCR4-35
pEF1/His B	pQE-32
pSinRep5	pMSCG19
pCAMBIA3200	pBT-5
pNIT-3	pCold-GST
pGFP22	pFA1

MSP2135	hCas9_D10A
pPBbsr2-4031NES	pSBbi-GN
pEGFP-NMHC II-C	Tcf/Lef:H2B-GFP
pHIV-Luc-ZsGreen	pHAGE2-TetOminiCMV-mCherry
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2	BCR/ABL P190 transgenic construct
pUCmini-iCAP-AAV.CAP-B22	pCRISPR-aBEST
pET21a-BirA	pNI3
pMDIAI	pc DNA FRT TO- APPSwed/Ind
pCas9	pGM1202
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T	TrkC-GFP
pCMV-SynA	Flag-TRIM24
pminiTol2	sfGFP-Cx43K258stop
pRJPaph-bjGFP	mTert-pBabe-puro
mTagBFP2-TOMM20-N-10	pCMV-2NLS-Cas9-6XMS2
pcDNA3.1-mCherry	pBFC1207
pHyo094 (eMutaT7)	Plasmid L5 attB::Pleft* mCherry
pRN11	pCI neo-hEST2
pDA077	pYLCRISPR/Cas9pUbi-N
jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcode	pRK GFP Paxillin
PG13-luc (wt p53 binding sites)	pcDNA3/Flag-METTL3
FUGW-PercevalHR	pLV-puro-N-3HA-SREBP1-C-Flag
pmGFP	pFE-sfGFP
pHAGE NFkB-TA-LUC-UBC-GFP-W	pEJS654 All-in-One AAV-sgRNA-hNmeCas9
pBabe-puro-miR-10b sponge	BECLIN-HA WT
pBECKP-km	pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-V
xCas9(3.6)-BE3	pMSCV-FlagMLL-pl-ENL(5613)
pmScarlet_Giantin_C1	pMaster1
CaV1.2	pGIPZ-PD-L1-EGFP
pSELECT-HA-mFOXO1	pBAD18-Cm
pSIN4-EF1a-LMO2-IRES-Puro	pC0061 PsmCas13 (B05) His6-TwinStrep-SUM
EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (F	pEcgRNA
pHAtC	pY001 (pFnCpf1_full)
pLentipuro3/TO/V5-GW/EGFP-Firefly Lucife	pCMV-HsPeg10rc3
pRK5-p18-HA	pCMVR8.74
pET15b_Bst-LF	pPH37
pAAV-EF1a-DIO-mCherry	pBS-hBAF60a
pLenti PGK Puro DEST (w529-2)	pGL3-mArg1 promoter/enhancer -31/-3810
pCAS9-TPC	Stat3 Flag pRc/CMV
DNMT3b KO Hyg	pR26 GFP Dest
pHIV-iRFP720-E2A-Luc	pBabe-hygro PyMT
pTNT-B18R-6His	pCMVHA hEZH2
pJQ200mp18	pGL3 2 kb prom. CD274
MSP680	cytoplasmic EKAR (Cerulean-Venus)
SIRT3 Flag	pMXs-hc-MYC
pAAV-EF1a-DIO-HTB	pNLF1W
pTK299	p415-GalL-Cas9-CYC1t
pHAGE2-TetOminiCMV-SKM	LentiGuide Cherry
pCAG Cas9-2A-Citrine	pF151 pcDNA3.1(+)SOD1WT
pCMVInt	pCMV-ABE7.9
pcDNA3.1 myc-NL-GW	pGL4-ERSE2-luc2P-Hygro

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pRS414

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