pProEX HTc

价格:900元

联系方式:I47-825O-882O

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pProEXHTc载体基本信息

出品公司: Invitrogen
别名: pProEX HTc, pProEX HTc
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4779bp
5' 测序引物序列: M13/pUC Reverse: AGCGGATAACAATTTCACACAGG (Invitrogen)
3' 测序引物序列:   pTrcHis Reverse: GATTTAATCTGTATCAGG (Invitrogen)
载体标签: N-His, N-TEV 蛋白酶切位点
载体抗性: 氨苄
备注:
Hosts: E.coli. Related vectors: pBR322.
产品目录号: 50611
稳定性: 瞬时表达 Transient
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pProEXHTc载体质粒图谱和多克隆位点信息

pProEX HTc载体图谱
pProEX HTc多克隆位点

pProEXHTc载体简介

pProEX HT系列原核表达系统是用来在大肠杆菌中表达外源蛋白的载体。使用该载体表达的目的蛋白含有6个His纯化标签。目的基因可以克隆到载体的多克隆位点处,可以选择的载体有pProEX HTa, b, c.蛋白表达后,His纯化标签就位于目的蛋白的N端,同时His纯化标签的使用可以用于蛋白纯化。载体上同时含有rTEV蛋白酶识别位点,可以使用rTEV蛋白酶进行酶切,从而去除融合的His标签。

pProEXHTc载体序列

LOCUS       pPROEX HTc	4779 bp 	DNA   circular	SYN
DEFINITION  pPROEX HTc
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="pPROEX HTc"
                     /mol_type="other DNA"
     promoter        193..266
                     /label="trc_promoter"
     misc_feature    235..257
                     /label="M13_pUC_rev_primer"
     misc_feature    complement(474..491)
                     /label="pTrcHis_rev_primer"
     misc_feature    complement(474..491)
                     /label="pBAD_rev_primer"
     terminator      524..681
                     /label="rrnB_terminator"
     terminator      647..690
                     /label="rrnB_T1_terminator"
     terminator      822..849
                     /label="rrnB_T2_terminator"
     promoter        891..919
                     /label="AmpR_promoter"
     gene            961..1821
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             961..1821
                     /label="ORF frame 1"
     rep_origin      1976..2595
                     /label="pBR322_origin"
     rep_origin      complement(2933..3239)
                     /label="f1_origin"
     misc_feature    3481..3503
                     /label="pGEX_3_primer"
     misc_feature    3650..4741
                     /label="lacI"
     CDS             3782..4741
                     /label="ORF frame 2"
ORIGIN
    1 GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC
   61 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC
  121 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC
  181 TGAAATGAGC TGTTGACAAT TAATCATCCG GTCCGTATAA TCTGTGGAAT TGTGAGCGGA
  241 TAACAATTTC ACACAGGAAA CAGACCATGT CGTACTACCA TCACCATCAC CATCACGATT
  301 ACGATATCCC AACGACCGAA AACCTGTATT TTCAGGGCGC CATGGGGATC CGGAATTCAA
  361 AGGCCTACGT CGACGAGCTC ACTAGTCGCG GCCGCTTTCG AATCTAGAGC CTGCAGTCTC
  421 GAGGCATGCG GTACCAAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA
  481 CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC
  541 GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT
  601 AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC
  661 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG
  721 TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG
  781 GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA
  841 TGGCCTTTTT GCGTTTCTAC AAACTCTTTT TGTTTATTTT TCTAAATACA TTCAAATATG
  901 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
  961 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
 1021 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
 1081 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
 1141 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
 1201 CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
 1261 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
 1321 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
 1381 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
 1441 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
 1501 CCTACAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
 1561 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
 1621 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
 1681 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
 1741 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
 1801 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
 1861 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
 1921 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
 1981 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
 2041 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
 2101 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
 2161 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
 2221 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
 2281 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
 2341 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
 2401 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
 2461 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
 2521 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
 2581 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
 2641 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
 2701 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA
 2761 GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATAATT
 2821 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA
 2881 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA
 2941 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC
 3001 GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG
 3061 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG
 3121 GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG
 3181 GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG
 3241 CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCTAT GGTGCACTCT CAGTACAATC
 3301 TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCAAT ATTGCTACGT GACTGGTACA
 3361 CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG
 3421 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT
 3481 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCAGA
 3541 TCAATTCGCG CGCGAAGGCG AAGCGGCATG CATTTACGTT GACACCATCG AATGGTGCAA
 3601 AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATGT
 3661 GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC
 3721 CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC
 3781 GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC
 3841 GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC
 3901 GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG
 3961 AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG
 4021 GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC
 4081 TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT
 4141 CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA
 4201 AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG
 4261 GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG
 4321 TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC
 4381 GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG
 4441 GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG ACAGCTCATG
 4501 TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT
 4561 GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT
 4621 CTCACTGGTG AAAAGAAAAA CCACCCTGGC ACCCAATACG CAAACCGCCT CTCCCCGCGC
 4681 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG
 4741 AGCGCAACGC AATTAATGTG AGTTAGCGCG AATTGATCT
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pProEXHTc载体应用举例

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