PinPoint Xa-2

价格:1350元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: PinPoint Xa-2
质粒类型: 大肠杆菌表达载体;蛋白纯化
表达水平:
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3332 bp
5' 测序引物及序列: Sp6 Rev
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: V2051
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

Pin point xa1载体图谱
Pin Point xa2多克隆位点

Pin Point xa2载体特征

载体简介

载体序列

LOCUS       PinPoint Xa-2	3332 bp 	DNA	SYN
DEFINITION  PinPoint Xa-2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="PinPoint Xa-2"
                     /mol_type="other DNA"
     misc_feature    1..386
                     /label="biotin_Pinpoint_Xa-1"
     CDS             1..489
                     /label="ORF frame 1"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
                     DT*"
     misc_feature    complement(452..469)
                     /label="Sp6_primer"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
                     DT*"
     misc_feature    669..691
                     /label="pGEX_3_primer"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
                     DT*"
     promoter        850..878
                     /label="AmpR_promoter"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
                     DT*"
     gene            920..1780
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
                     DT*"
     CDS             920..1780
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      1935..2554
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        2797..2815
                     /label="T7_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2939..2958
                     /label="pBRrevBam_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        3210..3238
                     /label="tac_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3250..3272
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
   61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
  121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
  181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
  241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
  301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
  361 atcggggatc tcgagctcat cgaaggtcgc gaaagcttca gctgggatcc ggtaccgata
  421 tcagatctcc cggggcggcc gcgatctggt tctatagtgt cacctaaatc gtatgtgtat
  481 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt ccatatggtg
  541 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
  601 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
  661 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
  721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
  781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
  841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
  901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
  961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
 1021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
 1081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
 1141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
 1201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
 1261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
 1321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
 1381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
 1441 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
 1501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
 1561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
 1621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
 1681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
 1741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
 1801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
 1861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
 1921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
 1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
 2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
 2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
 2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
 2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
 2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
 2341 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
 2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
 2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
 2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
 2581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
 2641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
 2701 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
 2761 gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact atagggagac
 2821 cggcctcgag cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
 2881 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
 2941 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
 3001 tgcgtccggc gtagaggatc gatccgggct tatcgactgc acggtgcacc aatgcttctg
 3061 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat
 3121 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg
 3181 gttctggcaa atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt
 3241 ggaattgtga gcggataaca atttcacaca ggaaacagaa ttcccagctt ggctgcagaa
 3301 ccattccatt cgttgatccg ggagtaactc ac
//

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