pLZ386

LOCUS       pLZ386         10765 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..191
                     /label=182-WKG-596-F
     primer_bind     235..251
                     /label=oMH18
     misc_feature    252..405
                     /label=Transcription blocker
                     /label=Transcription\blocker
     misc_feature    439..525
                     /label=CRE reg_Regulatory_Seq
     misc_feature    450..457
                     /label=CRE
     misc_feature    486..493
                     /label=CRE*
     misc_feature    508..515
                     /label=CRE
     promoter        532..534
                     /label=P_TAL
     misc_feature    554..640
                     /label=CRE reg_Regulatory_Seq
     misc_feature    565..572
                     /label=CRE
     misc_feature    601..608
                     /label=CRE*
     misc_feature    623..630
                     /label=CRE
     promoter        647..649
                     /label=P_TAL
     misc_feature    669..755
                     /label=CRE reg_Regulatory_Seq
     misc_feature    680..687
                     /label=CRE
     misc_feature    716..723
                     /label=CRE*
     misc_feature    738..745
                     /label=CRE
     promoter        762..908
                     /label=P_TAL
     primer_bind     865..892
                     /label=180-WKG-592-F
     protein_bind    complement(924..957)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          1778..1793
                     /label=SV40 int
     intron          1779..1844
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1974..1994
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRRV"
     polyA_signal    complement(2358..2549)
                     /label=SV40 late polyA
     polyA_signal    2590..2814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      2823..3007
                     /label=SV40 late polyA
     misc_feature    3044..3077
                     /label=loxp
     protein_bind    complement(3044..3077)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3084..3132
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3141
                     /label=T7
     regulatory      3159..3168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3167..3192)
                     /label=199-WKG-625-R
     CDS             3168..3866
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    3883..3901
                     /label=linker
     CDS             3903..4601
                     /codon_start=1
                     /label=GNCR
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     CDS             4602..4631
                     /codon_start=1
                     /label=linker
                     /translation="ASGSGGGGDV"
     misc_feature    4633..4636
                     /label=linker
     CDS             4638..5336
                     /codon_start=1
                     /label=GNCR-2
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     CDS             5337..5366
                     /codon_start=1
                     /label=linker
                     /translation="ASGSGGGGDV"
     misc_feature    5368..5372
                     /label=linker
     misc_feature    5373..6315
                     /label=NSP3
     terminator      6359..6586
                     /label=bGH polyA
     rep_origin      6632..7060
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     promoter        7074..7403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7254..7389
                     /label=SV40 ORI
     misc_feature    7306..7355
                     /label=P_SV40 TSS-region
     misc_feature    7470..8261
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     polyA_signal    8438..8559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8604..8624)
                     /label=M13-rev
     primer_bind     complement(8608..8624)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(8630..8652)
                     /label=LacO
     protein_bind    8632..8648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8656..8686)
                     /label=lac promoter
     protein_bind    8701..8722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8828..9768
                     /label=ColE1
                     /note="ColE1"
     misc_feature    complement(9772..10629)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(10630..10734)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggtactt
      241 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
      301 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
      361 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga taggtaccga
      421 gctcttacgc gtgctagcgc accagacagt gacgtcagct gccagatccc atggccgtca
      481 tactgtgacg tctttcagac accccattga cgtcaatggg agaacagatc tgccagctct
      541 tacgcgtgct agcgcaccag acagtgacgt cagctgccag atcccatggc cgtcatactg
      601 tgacgtcttt cagacacccc attgacgtca atgggagaac agatctgcca gctcttacgc
      661 gtgctagcgc accagacagt gacgtcagct gccagatccc atggccgtca tactgtgacg
      721 tctttcagac accccattga cgtcaatggg agaacagatc tgccgccccg actgcatctg
      781 cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca tagaactaaa
      841 gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac gggccacggg
      901 gatgaagcag aaggctagcg cttataactt cgtatagcat acattatacg aagttatcgg
      961 cgcgccattg gctgcagggt cgctcggtgt tcgaggccac acgcgtcacc ttaatatgcg
     1021 aagtggacct gggaccgcgc cgccccgact gcatctgcgt gttcgaattc gccaatgaca
     1081 agacgctggg cggggtttgc tcgacattgg gtggaaacat tccaggcctg ggtggagagg
     1141 ctttttgctt cctcttgcaa aaccacactg ctcgacattg ggtggaaaca ttccaggcct
     1201 gggtggagag gctttttgct tcctcttgaa aaccacactg ctcgactagt gattaaagtc
     1261 gactcgggga caccaaatat ggcgatctcg gccttttcgt ttcttggagc tgggacatgt
     1321 ttgccatcga tccatctacc accagaacgg ccgttagatc tgctgccacc gttgtttcca
     1381 ccgaagaaac caccgttgcc gtaaccacca cgacggttgt tgctaaagaa gctgccaccg
     1441 ccacggccac cgttgtagcc gccgttgttg ttattgtagt tgctactgtt atttctggca
     1501 cttcttggtt ttcctcttaa gtgaggagga acataaccat tctcgttgtt gtcgttgatg
     1561 cttaaatttt gcacttgttc gctcagttca gccataatat gaaatgcttt tcttgttgtt
     1621 cttacggaat accacttgcc acctatcacc acaactaact ttttcccgtt cctccatctc
     1681 ttttatattt tttttctcga gggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     1741 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     1801 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     1861 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     1921 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     1981 agaagagaag ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     2041 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     2101 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     2161 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     2221 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     2281 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     2341 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acatagaatg
     2401 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2521 aaactcatca atgtatctta tcatgtctgg atctgacatg gtaagtcctg cagggctgat
     2581 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2641 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2701 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2761 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     2821 aggataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2881 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2941 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     3001 gttttttaaa gcgcttggta ccgtctttcc agtggttaat taaataactt cgtatagcat
     3061 acattatacg aagttatcga tccgaacaaa cgacccaaca cccgtgcgtt ttattctgtc
     3121 tttttattgc cgatcctcga gagacccaag ctggctaggc caccatggac atcgagaagc
     3181 tgtgcaagaa ggcggaagag gaggccaagg aggcccagga gaaggccgac gagctgagac
     3241 aacgacaccc cgattcgcag gcggcggaag acgcggagga tctcgcaaat ctggctgtcg
     3301 ccgccgtgct gaccgcttgt ctgctggccc aggagcatcc caacgccgat atcgctaagc
     3361 tgtgcatcaa ggccgcgagc gaagctgcgg aggccgcgtc aaaagccgcc gagctggccc
     3421 aacggcaccc agacagtcaa gcagccagag atgctatcaa actcgcctct caagccgccc
     3481 gcgccgtgat cctggccatc atgctcgccg cagagaatcc caacgctgat attgcaaagc
     3541 tatgtattaa ggcggccagc gaagccgcag aggctgcctc gaaagctgcg gaacttgcac
     3601 agcggcaccc cgactcccag gccgcccgag atgcaattaa actggccagt caggccgctg
     3661 aggcggtgga gagagccatc tggctggccg ccgagaaccc caatgccgat atcgccaaaa
     3721 aatgcatcaa agcggcctcc gaagcagccg aggaggcctc taaagctgcc gaagaggctc
     3781 aaagacaccc agacagccag aaggcacgcg acgagatcaa ggaggcctct cagaaggccg
     3841 aggaggtgaa ggagagatgc aagagcgcca gcggaagtgg cggaggaggc gacgtcgcta
     3901 gtgacattga gaagctctgt aagaaagccg aagaagaggc taaagaagca caggagaaag
     3961 ctgatgagtt gcggcagcgg cacccggatt cacaagcggc cgaagacgca gaggacctgg
     4021 ccaacctggc cgtagccgcg gttctgaccg catgtctgct cgcccaagag catcctaacg
     4081 cagatatcgc taagctgtgc atcaaggcag cgtccgaggc cgccgaggcc gcaagtaaag
     4141 ccgctgaatt agcacaaagg catccagaca gtcaggccgc aagggacgca atcaaactgg
     4201 catctcaggc tgccagagcg gtgatccttg caattatgct ggccgccgaa aacccaaatg
     4261 ctgatatagc aaaactgtgc atcaaagcag ccagcgaagc agcggaagcc gcttccaagg
     4321 ccgctgagtt ggcccaaaga catccagata gccaagcagc ccgtgatgcg atcaaactcg
     4381 ctagtcaggc tgccgaggcc gttgaacgtg ctatatggct ggcggccgaa aatccaaatg
     4441 ccgacatagc caaaaagtgt atcaaggccg cctcagaagc tgccgaagag gcgagcaagg
     4501 ccgcagaaga ggcccagcgc catcccgatt cccagaaggc ccgggatgag atcaaagaag
     4561 ccagccagaa agctgaggag gtcaaagagc gatgtaagag tgcgagcgga agtggcggtg
     4621 gcggtgacgt ggctagtgat atcgagaagt tgtgtaaaaa agctgaggaa gaggcaaaag
     4681 aggcccagga aaaggcagac gagcttcgtc agagacatcc tgattcccag gccgcggagg
     4741 atgctgagga cctcgccaat ttggccgtcg cagctgtgct gacggcctgc ctccttgcgc
     4801 aagaacaccc taatgcggac atagctaaac tctgcattaa ggccgcctca gaggccgcag
     4861 aagccgcttc aaaagctgcc gaactagcac agagacatcc tgactctcag gcagctagag
     4921 acgctattaa gcttgcttct caagccgccc gagccgttat cctggccatt atgctagccg
     4981 cagagaatcc gaacgccgac atcgcaaaac tgtgcataaa ggccgcatct gaggcagcag
     5041 aggcagccag taaggccgct gaactcgcac agaggcaccc tgattcacag gcagcgcggg
     5101 atgctattaa gttagcttca caggctgccg aagctgtgga gagagccatt tggcttgctg
     5161 cggaaaatcc aaatgccgac atcgccaaga aatgcatcaa agctgcctcc gaggcggccg
     5221 aagaagccag taaagctgca gaggaagctc agcgccaccc tgactctcag aaagcgcggg
     5281 acgagattaa agaggcaagc cagaaagcag aagaggtcaa agagcgctgt aagagcgcta
     5341 gtggctccgg aggcggcggc gacgtcgcta gcatgctcaa gatggagtct acacagcaga
     5401 tggctagttc gattattaac acttcttttg aagccgcagt cgttgctgcc acttcaactc
     5461 ttgaacttat gggcattcaa tatgattata atgaagttta tactagagtt aagagcaaat
     5521 ttgattacgt aatggatgat tcaggtgtta aaaacaatct gttgggtaaa gcggcaacta
     5581 ttgatcaggc actgaatgga aaatttggtt cagcagcaag aaatagaaat tggatggctg
     5641 atactagaac tacagctaga ctagatgaag atgtgaacaa acttagaatg atgctatcat
     5701 ctaaaggaat tgatcaaaaa atgagagtac ttaatgcatg tttcaatgtg aagcgagttc
     5761 caggtaaatc atcatcaata attaaatgta ctagattaat gagagataag attgaacgtg
     5821 gtgaagtcga agtggatgat tcgtttgttg aagaaaaaat ggaagttgac acaattgatt
     5881 ggaaatctag atatgaacaa ttggaaaaac gatttgaatc gctgaaacaa agggtgaatg
     5941 aaaaatatac aagttgggtg caaaaagcaa aaaaggtaaa tgaaaatatg tactctctcc
     6001 aaaacgttat atcacaacag cagagccaga ttgctgattt acaaaattac tgtaataaat
     6061 tggaagttga tctacaaaat aaaattagtt cacttgtgtc ttcagtcgaa tggtacctga
     6121 aatcaatgga attgcctgat gaaattaaga cagatattga acaacaacta aattccattg
     6181 atgtaatcaa tccaattaat gctatagatg attttgaatc actgatcaga aatattatac
     6241 tcgactatga cagaatattt ttaatgttta aaggattaat gaggcaatgc aactatgaat
     6301 atacgtatga ataggatcca agcttctaga gggcccgttt aaacccgctg atcagcctcg
     6361 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6421 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6481 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6541 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6601 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7081 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7321 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7381 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7441 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7501 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7561 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7621 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     7681 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7741 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7801 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7861 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7921 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7981 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     8041 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8101 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8161 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8221 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8281 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8341 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8401 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     8581 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8641 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8701 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8761 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8821 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8881 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8941 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9001 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9061 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9121 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9181 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9241 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9301 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9361 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9421 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9481 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9541 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9601 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9661 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9721 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9781 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9841 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9901 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9961 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10021 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10081 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10141 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10201 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10261 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10321 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10381 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10441 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10501 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10561 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10621 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10681 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10741 tccccgaaaa gtgccacctg acgtc
//