| 质粒名称: | pSMVP-Cas9C |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80939 |
| 存储实验室: | George Church |
|
载体骨架基本信息 |
|
| 载体名称: | pMAX |
| 载体出品公司: | Lonza |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 2900 bp |
| 完整质粒大小: | 5067 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，CRISPR，合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9C,Split-Cas9 C-terminal half |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSMVP-Cas9C was a gift from George Church (Addgene plasmid # 80939 ; http://n2t.net/addgene:80939 ; RRID:Addgene_80939) |
| 参考文献部分: | A multifunctional AAV-CRISPR-Cas9 and its host response. Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, Wagers AJ, Church GM. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. 10.1038/nmeth.3993 PubMed 27595405 |
买家导航
pSMVPCas9C载体基本信息
pSMVPCas9C载体图谱质粒图谱和多克隆位点信息
pSMVPCas9C载体简介
pSMVPCas9C载体序列
LOCUS pSMVP-Cas9C 5067 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid that expresses Cas9C in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS pSMVP-Cas9C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5067)
AUTHORS Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K,
Wagers AJ, Church GM
TITLE A multifunctional AAV-CRISPR-Cas9 and its host response.
JOURNAL Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
PUBMED 27595405
REFERENCE 2 (bases 1 to 5067)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5067
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(39..59)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
enhancer 289..668
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 669..872
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 823..843
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
intron 964..1096
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
regulatory 1126..1135
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 1130..1139
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1736..1747
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 3265..3285
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(3296..3315)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
polyA_signal 3325..3406
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 3350..3369
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin complement(3604..4192)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3684..3703)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4273..5067)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4384..4403)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 4994..5013
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
ORIGIN
1 aatattattg aagcatttat cagggttcgt ctcgctgtgg aatgtgtgtc agttagggtg
61 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
121 agcaaccagt cccggtctcc tcccatgcat gtcaatattg gccattagcc atattattca
181 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
241 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
301 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
541 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
601 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
661 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
721 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
781 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
841 gtgtacggtg ggaggtctat ataagcagag gtcgtttagt gaaccgtcag atcactagta
901 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct cgactgatca
961 caggtaagta tcaaggttac aagacaggtt taaggaggcc aatagaaact gggcttgtcg
1021 agacagagaa gattcttgcg tttctgatag gcacctattg gtcttactga catccacttt
1081 gcctttctct ccacaggggt accgaagccg ctagcgctac cggtcgccac catggcggcg
1141 gcgtgcccgg aactgcgtca gctggcgcag agcgatgtgt attgggatcc gattgtgagc
1201 attgaaccgg atggcgtgga agaagtgttt gatctgaccg tgccgggccc gcataacttt
1261 gtggcgaacg atattattgc gcataactct ggccaggggg acagtcttca cgagcacatc
1321 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg
1381 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc
1441 cgagagaacc aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt
1501 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc
1561 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg
1621 gatcaggaac tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag
1681 tcttttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaataga
1741 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg
1801 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa
1861 cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca
1921 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat
1981 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca
2041 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg
2101 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt
2161 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag
2221 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat
2281 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa
2341 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg
2401 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc
2461 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa
2521 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg
2581 gttgtggcca aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg
2641 ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg
2701 aaaggatata aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt
2761 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac
2821 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag
2881 ctcaaagggt ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac
2941 taccttgatg agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac
3001 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag
3061 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc
3121 aagtacttcg acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac
3181 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag
3241 ctcggtggag acagcagggc tgaccccaag aagaagagga aggtgtctcg agctcgatga
3301 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
3361 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
3421 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
3481 cctctacaaa tgtggtactt aagaggggga gaccaaaggg cgagacgtta aggcctcacg
3541 tgacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
3601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
3661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
3721 gcgctctcct gttccgaccc tgccgcttac gggatacctg tccgcctttc tcccttcggg
3781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
3841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
3901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
3961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
4021 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
4081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
4141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
4201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
4261 ggtcatgccg tctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
4321 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
4381 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
4441 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
4501 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
4561 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
4621 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
4681 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
4741 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
4801 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
4861 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt
4921 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
4981 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
5041 tgcgtgcaat ccatcttgtt caatcat
//
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