pLZ74

LOCUS       pLZ74                8557 bp DNA     circular SYN 13-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     643..668
                     /label=185-WKG-602-CMV-F
     primer_bind     863..882
                     /label=T7
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      900..909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             909..1607
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    1624..1642
                     /label=linker
     CDS             1644..2342
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    2359..2377
                     /label=linker
     CDS             2379..3077
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    3094..3113
                     /label=linker
     misc_feature    3114..4055
                     /label=NSP3
     misc_feature    3114..3127
                     /label=NSP3
     primer_bind     complement(3125..3144)
                     /label=179-WKG-592-R
     polyA_signal    4154..4378
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4424..4852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4866..5195
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    5046..5123
                     /label=SV40 ORI
     CDS             5262..6056
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6230..6351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6396..6416)
                     /label=M13-rev
     primer_bind     complement(6400..6416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6422..6444)
                     /label=LacO
     protein_bind    6424..6440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6448..6478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(6449..6478)
                     /label=lac
     protein_bind    6493..6514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6620..7560
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7561..8421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8422..8526)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagg
      901 ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag gaggcccagg
      961 agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa gacgcggagg
     1021 atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc caggagcatc
     1081 ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg gaggccgcgt
     1141 caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga gatgctatca
     1201 aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc gcagagaatc
     1261 ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca gaggctgcct
     1321 cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga gatgcaatta
     1381 aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc gccgagaacc
     1441 ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc gaggaggcct
     1501 ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc gacgagatca
     1561 aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc agcggaagtg
     1621 gcggaggagg cgacgtcgct agtgacatcg agaagctgtg caagaaggcg gaagaggagg
     1681 ccaaggaggc ccaggagaag gccgacgagc tgagacaacg acaccccgat tcgcaggcgg
     1741 cggaagacgc ggaggatctc gcaaatctgg ctgtcgccgc cgtgctgacc gcttgtctgc
     1801 tggcccagga gcatcccaac gccgatatcg ctaagctgtg catcaaggcc gcgagcgaag
     1861 ctgcggaggc cgcgtcaaaa gccgccgagc tggcccaacg gcacccagac agtcaagcag
     1921 ccagagatgc tatcaaactc gcctctcaag ccgcccgcgc cgtgatcctg gccatcatgc
     1981 tcgccgcaga gaatcccaac gctgatattg caaagctatg tattaaggcg gccagcgaag
     2041 ccgcagaggc tgcctcgaaa gctgcggaac ttgcacagcg gcaccccgac tcccaggccg
     2101 cccgagatgc aattaaactg gccagtcagg ccgctgaggc ggtggagaga gccatctggc
     2161 tggccgccga gaaccccaat gccgatatcg ccaaaaaatg catcaaagcg gcctccgaag
     2221 cagccgagga ggcctctaaa gctgccgaag aggctcaaag acacccagac agccagaagg
     2281 cacgcgacga gatcaaggag gcctctcaga aggccgagga ggtgaaggag agatgcaaga
     2341 gcgccagcgg aagtggcgga ggaggcgacg tcgctagtga catcgagaag ctgtgcaaga
     2401 aggcggaaga ggaggccaag gaggcccagg agaaggccga cgagctgaga caacgacacc
     2461 ccgattcgca ggcggcggaa gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc
     2521 tgaccgcttg tctgctggcc caggagcatc ccaacgccga tatcgctaag ctgtgcatca
     2581 aggccgcgag cgaagctgcg gaggccgcgt caaaagccgc cgagctggcc caacggcacc
     2641 cagacagtca agcagccaga gatgctatca aactcgcctc tcaagccgcc cgcgccgtga
     2701 tcctggccat catgctcgcc gcagagaatc ccaacgctga tattgcaaag ctatgtatta
     2761 aggcggccag cgaagccgca gaggctgcct cgaaagctgc ggaacttgca cagcggcacc
     2821 ccgactccca ggccgcccga gatgcaatta aactggccag tcaggccgct gaggcggtgg
     2881 agagagccat ctggctggcc gccgagaacc ccaatgccga tatcgccaaa aaatgcatca
     2941 aagcggcctc cgaagcagcc gaggaggcct ctaaagctgc cgaagaggct caaagacacc
     3001 cagacagcca gaaggcacgc gacgagatca aggaggcctc tcagaaggcc gaggaggtga
     3061 aggagagatg caagagcgcc agcggaagtg gcggaggagg cgacgtcgct agcatgctca
     3121 agatggagtc tacacagcag atggctagtt cgattattaa cacttctttt gaagccgcag
     3181 tcgttgctgc cacttcaact cttgaactta tgggcattca atatgattat aatgaagttt
     3241 atactagagt taagagcaaa tttgattacg taatggatga ttcaggtgtt aaaaacaatc
     3301 tgttgggtaa agcggcaact attgatcagg cactgaatgg aaaatttggt tcagcagcaa
     3361 gaaatagaaa ttggatggct gatactagaa ctacagctag actagatgaa gatgtgaaca
     3421 aacttagaat gatgctatca tctaaaggaa ttgatcaaaa aatgagagta cttaatgcat
     3481 gtttcaatgt gaagcgagtt ccaggtaaat catcatcaat aattaaatgt actagattaa
     3541 tgagagataa gattgaacgt ggtgaagtcg aagtggatga ttcgtttgtt gaagaaaaaa
     3601 tggaagttga cacaattgat tggaaatcta gatatgaaca attggaaaaa cgatttgaat
     3661 cgctgaaaca aagggtgaat gaaaaatata caagttgggt gcaaaaagca aaaaaggtaa
     3721 atgaaaatat gtactctctc caaaacgtta tatcacaaca gcagagccag attgctgatt
     3781 tacaaaatta ctgtaataaa ttggaagttg atctacaaaa taaaattagt tcacttgtgt
     3841 cttcagtcga atggtacctg aaatcaatgg aattgcctga tgaaattaag acagatattg
     3901 aacaacaact aaattccatt gatgtaatca atccaattaa tgctatagat gattttgaat
     3961 cactgatcag aaatattata ctcgactatg acagaatatt tttaatgttt aaaggattaa
     4021 tgaggcaatg caactatgaa tatacgtatg aataggatcc actagtccag tgtggtggaa
     4081 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
     4141 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4201 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4261 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4321 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     4381 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4441 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4501 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4561 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4621 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4681 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4741 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4801 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     4861 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4921 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4981 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5041 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5101 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5161 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     5221 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5281 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5341 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5401 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5461 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5521 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5581 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5641 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5701 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5761 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     5821 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5881 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5941 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6001 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6061 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     6121 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6181 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6361 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6421 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6481 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6541 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8521 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//