| 质粒名称: | pBiFC-bJunVN155(I152L) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27098 |
| 存储实验室: | Chang-Deng Hu |
|
载体骨架基本信息 |
|
| 载体名称: | pMyc-CMV |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 3791 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | bJunVN155(I152L) |
| 物种来源: | R.家鼠（大鼠）；维多利亚水母 |
| 插入大小: | 689 bp |
| 基因突变: | I152L in Venus(1-154) |
| 标签/融合蛋白: | Myc（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | n/a |
| 3' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | pBiFC-bJunVN155(I152L) was a gift from Chang-Deng Hu (Addgene plasmid # 27098) |
| 参考文献部分: | An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Nov . 49(5):793-805. 10.2144/000113519 PubMed 21091444 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pBiFC-bJunVN155(I152L) 4457 bp DNA SYN
DEFINITION pBiFC-bJunVN155(I152L)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4457
/organism="pBiFC-bJunVN155(I152L)"
/mol_type="other DNA"
CDS 1..756
/label="ORF frame 1"
misc_feature 4..27
/label="Myc_primer"
gene 292..735
/label="EGFP (0 - 443)"
/gene="EGFP (0 - 443)"
misc_feature complement(334..355)
/label="EGFP_N_primer"
terminator complement(778..897)
/label="SV40_PA_terminator"
misc_feature complement(792..811)
/label="EBV_rev_primer"
promoter complement(1009..1027)
/label="M13_reverse_primer"
misc_feature complement(1026..1048)
/label="M13_pUC_rev_primer"
promoter complement(1062..1091)
/label="lac_promoter"
gene complement(2174..3034)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2174..3034)
/label="ORF frame 2"
promoter complement(3076..3104)
/label="AmpR_promoter"
misc_feature complement(3263..3285)
/label="pGEX_3_primer"
misc_feature complement(3484..3627)
/label="lacZ_a"
misc_feature 3598..3620
/label="M13_pUC_fwd_primer"
promoter 3613..3629
/label="M13_forward20_primer"
misc_feature 3660..3947
/label="CAG_enhancer"
promoter 3665..4157
/label="CMV_immearly_promoter"
misc_feature 4114..4134
/label="CMV_fwd_primer"
promoter 4115..4184
/label="CMV_promoter"
promoter 4127..4246
/label="CMV2_promoter"
misc_feature 4158..4177
/label="pCEP_fwd_primer"
misc_feature 4160..4184
/label="LNCX_primer"
misc_feature 4260..4286
/label="pCMV_primer"
misc_feature 4301..4397
/label="modSV40_late_16s_int"
misc_feature 4301..4331
/label="SV40_late_19s_int"
ORIGIN
1 ATGGCATCAA TGCAGAAGCT GATCTCAGAG GAGGACCTGC TTATGGCCAT GGAGGCCCGA
61 ATTCGGAAGG CGGAGAGGAA GCGCATGAGG AACCGCATCG CTGCCTCCAA GTGCCGGAAA
121 AGGAAGCTGG AGCGGATCGC CCGGCTAGAG GAAAAAGTGA AAACCTTGAA AGCGCAAAAC
181 TCCGAGCTGG CGTCCACGGC CAACATGCTC AGGGAACAGG TGGCACAGCT TAAACAGAAA
241 GTCATGAACC ACGGTACCGG AGGTGGCGGG AGCGGAGGTG GCGGGAGTAT GGTGAGCAAG
301 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
361 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
421 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
481 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
541 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
601 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
661 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
721 AACTACAACA GCCACAACGT CTATCTCACC GCCTGAGCGG CCGCGGGGAT CCAGACATGA
781 TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA
841 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG
901 TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT
961 TTTCGGATCC TCTAGAGTCG ATCTGCAGGC ATGCTAGCTT GGCGTAATCA TGGTCATAGC
1021 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA
1081 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT
1141 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC
1201 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
1261 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
1321 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
1381 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
1441 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
1501 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
1561 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
1621 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
1681 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
1741 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
1801 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG
1861 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
1921 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
1981 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
2041 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
2101 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
2161 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
2221 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
2281 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT
2341 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT
2401 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
2461 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG
2521 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
2581 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG
2641 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG
2701 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
2761 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA
2821 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
2881 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
2941 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
3001 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
3061 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
3121 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA
3181 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC
3241 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT
3301 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG
3361 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA
3421 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC
3481 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC
3541 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC
3601 AGTCACGACG TTGTAAAACG ACGGCCAGTG AGTTCGAGCT TGCATGCCTG CAGGTCGTTA
3661 CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT
3721 CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG
3781 TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA
3841 CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA
3901 CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG
3961 TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC
4021 CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT
4081 TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT
4141 GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC
4201 CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG ACTCTAGAGG
4261 ATCCGGTACT AGAGGAACTG AAAAACCAGA AAGTTAACTG GTAAGTTTAG TCTTTTTGTC
4321 TTTTATTTCA GGTCCCGGAT CCGGTGGTGG TGCAAATCAA AGAACTGCTC CTCAGTGGAT
4381 GTTGCCTTTA CTTCTAGGCC TGTACGGAAG TGTTACTTCT GCTCTAAAAG CTGCGGAATT
4441 GTACCCGCGG GCCCACC
//
